Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
Molecular Oncology, EarlyView.We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
Fabrication and Performance of a Ta2O5 Thin Film pH Sensor Manufactured Using MEMS Processes. [PDF]
Sensors (Basel), 2023 Guo Y +7 more
europepmc +1 more source
USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival
Molecular Oncology, EarlyView.CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna +8 more
wiley +1 more source
The New Reliable pH Sensor Based on Hydrous Iridium Dioxide and Its Composites. [PDF]
Materials (Basel), 2022 Lenar N, Piech R, Paczosa-Bator B.
europepmc +1 more source
Journal of Engineering and Sustainable Development, 2016 This work presents the fabrication, characterization and performance analysis of Zinc Oxide (ZnO) and Palladium doped Zinc Oxide (Pd-ZnO) thin films based Extended-Gate Field-Effect Transistor (EGFET) pH sensor.
Raad Hamdan Thaher +2 more
doaj
Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer
Molecular Oncology, EarlyView.New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau +9 more
wiley +1 more source
Redox-Active Green Electrode: Plant-Based Betanin Immobilized on Carbon Black for Drift-Free Voltammetric and Potentiometric pH Sensor Applications. [PDF]
ACS Omega, 2023 Meena J, Santhakumar K, Senthil Kumar A.
europepmc +1 more source
Attitudes of cancer patients toward ctDNA‐based liquid biopsy for follow‐up
Molecular Oncology, EarlyView.This mixed‐methods study explored cancer patients' experiences, knowledge, and preferences regarding ctDNA‐based liquid biopsy for follow‐up. Awareness of ctDNA was low and recall of previous liquid biopsy was imperfect, yet acceptance was high.
Siddharth Chhajlani +7 more
wiley +1 more source
Bioadhesive Gauze Embedded with Chitosan-Butein Bioconjugate: A Redox-Active pH Sensor Platform. [PDF]
Biosensors (Basel), 2022 Krishnan V +4 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source