Results 81 to 90 of about 6,503 (167)
Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis
Polyhydroxyalkanoic acids (PHA) represent a complex class of storage polyesters that are synthesized by a wide range of different Gram-positive and Gram-negative bacteria as well as by some Archaea and that are deposited as insoluble cytoplasmic ...
Steinbuchel, A +3 more
core +1 more source
SELEÇÃO POLIFÁSICA DE MICRORGANISMOS PRODUTORES DE POLIHIDROXIALCANOATOS
Polyhydroxyalkanoates (PHAs) are fully biodegradable biopolyesters produced by many prokaryotes and accumulated as insoluble cytoplasmatic inclusions. The detection of these intracellular granule is usually provided using lipofilic azodyes, which are not
Amanda L. S. Silva +3 more
doaj +1 more source
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 [PDF]
In this study, a transformant of C. necator PHB-4 harboring the PHA synthase gene of Chromobacterium sp. USM2 [C.necator PHB 4 (phaCcs)] was evaluated for its ability to synthesize novel short-chain and medium-chain-length (SCL-MCL) PHA containing ...
Ooi , Tin Fong, Ooi, Tin Fong
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Biotechnological production of PHA by selected thermophilic bacteria
This thesis is mainly focused on the production of PHA copolymers and study of metabolism of the thermophilic genus Aneurinibacillus. Theoretical part mainly deals with general characterization and production of PHA and with PHA producing bacteria ...
Marková, Lucie
core
Molecular characterization of selected PHA producers
This diploma thesis focuses on the molecular characterization of selected PHA producers. Within this work, the PHA producing thermophilic isolates originating from the samples of activated sludge and compost were identified and characterized using ...
Kubáčková, Eliška
core +1 more source
Título en ingles: Analysis of polyhydroxyalkanoate synthases (PhaC1 and PhaC2) in a strain of Pseudomonas fluorescens IBUN S1602 isolated from Colombian soil.
Julieth Serrano Riaño +3 more
doaj
รายงานฉบับสมบูรณ์เรื่อง การโคลนจีนที่สร้างโพลีไฮดรอกซีอัลคาโนเอท (PHA) ของ Rhodopseudomonas palustris สายพันธุ์ NCIB8288 เข้าไปยัง Escherichia coli [PDF]
จากการทดลองการหาสภาวะที่เหมาะสมในการสกัด PHA synthase ออกจากเซลล์ พบว่าสภาวะที่เหมาะสมในการสกัดเอนไซม์ PHA synthase จากเซลล์ คือการทำให้เซลล์แตกด้วย sonicator โดยใช้ช่วงเวลา 5 นาที โดยให้หยุดทุก ๆ 15 วินาที เป็นเวลา 5 วินาที ค่ากิจกรรมของ PHA synthase ...
สมพร ตั๊นสกุล
core
本實驗室先前藉由蛋白質工程-區域性半隨機性突變方式(localized semirandom mutagenesis)引入十四個突變點,成左滷NPseudomonas putida Gpo1聚羥基烷酸酯酵素(PHA synthase1;Class II)的酵素基質專一性拓展開來(Sheu and Lee , 2004)。本實驗的目的是藉由建立定點突變的技術,配合廣宿主共表現載體分析平台,轉型入PHA累積菌株Pseudomonas putida GPp104,以礦物鹽培養基加入辛酸鹽(octanoate ...
Tsai, Pei-Chien, 蔡佩倩
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