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Asymmetric Open-Closed Dimer Mechanism of Polyhydroxyalkanoate Synthase PhaC [PDF]

iScience, 2020
Summary: Biodegradable polyester polyhydroxyalkanoate (PHA) is a promising bioplastic material for industrial use as a replacement for petroleum-based plastics.
Min Fey Chek, Sun-Yong Kim, Tomoyuki Mori, Hua Tiang Tan, Kumar Sudesh, Toshio Hakoshima   +5 more
doaj   +5 more sources

Engineering of Aeromonas caviae Polyhydroxyalkanoate Synthase Through Site-Directed Mutagenesis for Enhanced Polymerization of the 3-Hydroxyhexanoate Unit [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Polyhydroxyalkanoate (PHA) synthase is an enzyme that polymerizes the acyl group of hydroxyacyl-coenzyme A (CoA) substrates. Aeromonas caviae PHA synthase (PhaCAc) is an important biocatalyst for the synthesis of a useful PHA copolymer, poly[(R)-3 ...
Ken Harada, Ken Harada, Shingo Kobayashi, Kanji Oshima, Shinichi Yoshida, Takeharu Tsuge, Shunsuke Sato   +6 more
doaj   +4 more sources

Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers [PDF]

FEBS Open Bio
The rapid escalation of global plastic consumption, coupled with the environmental impacts of petrochemical polymers, has sparked a surge of interest in bioplastics, particularly those derived from microbial and enzymatic processes.
Giovanni Gallo, Emma Piccoli, Luca Bombardi, Martina Aulitto, Salvatore Fusco   +4 more
doaj   +3 more sources

Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4 [PDF]

Microorganisms, 2022
The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of
Saki Goto, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, Ayaka Hiroe   +4 more
doaj   +2 more sources

Screening Method for Polyhydroxyalkanoate Synthase Mutants Based on Polyester Degree of Polymerization Using High-Performance Liquid Chromatography [PDF]

Microorganisms, 2021
A high-throughput screening method based on the degree of polymerization (DP) of polyhydroxyalkanoate (PHA) was developed using high-performance liquid chromatography (HPLC).
Manami Ishii-Hyakutake, Tetsuo Sakurai, Takeharu Tsuge   +2 more
doaj   +2 more sources

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaCAR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of
Shuzo Arai, Sayaka Sakakibara, Robin Mareschal, Robin Mareschal, Toshihiko Ooi, Manfred Zinn, Ken’ichiro Matsumoto   +6 more
doaj   +2 more sources

Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions [PDF]

Microbial Cell Factories, 2017
Background Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins.
Jinping Du, Bernd H. A. Rehm
doaj   +2 more sources

Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome [PDF]

Data in Brief, 2018
We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia.
Tan Suet May Amelia, Al-Ashraf Abdullah Amirul, Kesaven Bhubalan   +2 more
doaj   +2 more sources

Heterologous expression and characterization of polyhydroxyalkanoate synthase genes from haloarchaeal strains in Haloferax mediterranei [PDF]

Frontiers in Microbiology
IntroductionPolyhydroxyalkanoates (PHAs) are promising materials for building a sustainable society due to their excellent biodegradability. Some haloarchaea, which require high salt concentrations for growth, possess class III PHA synthases (PhaECs) and
Keisuke Wada, Kazunori Ushimaru, Shun Sato, Tokuma Fukuoka   +3 more
doaj   +2 more sources

Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon Haloferax mediterranei: Key Enzyme of Biodegradable Plastic Synthesis [PDF]

Bioengineering
The biosynthesis of polyhydroxyalkanoate (PHA) biopolymer is highly dependent on the activity of a key enzyme, PHA synthase (PhaC). The halophilic archaeon Haloferax mediterranei can accumulate large amounts of PHAs from different carbon sources under ...
Diya Alsafadi, Yomen Ghalawinji, Fawwaz I. Khalili   +2 more
doaj   +2 more sources