Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions [PDF]
Microbial Cell Factories, 2017 Background Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins.
Jinping Du, Bernd H. A. Rehm
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Asymmetric Open-Closed Dimer Mechanism of Polyhydroxyalkanoate Synthase PhaC [PDF]
iScience, 2020 Summary: Biodegradable polyester polyhydroxyalkanoate (PHA) is a promising bioplastic material for industrial use as a replacement for petroleum-based plastics.
Min Fey Chek +5 more
doaj +5 more sources
Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome [PDF]
Data in Brief, 2018 We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia.
Tan Suet May Amelia +2 more
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Engineering of Aeromonas caviae Polyhydroxyalkanoate Synthase Through Site-Directed Mutagenesis for Enhanced Polymerization of the 3-Hydroxyhexanoate Unit [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Polyhydroxyalkanoate (PHA) synthase is an enzyme that polymerizes the acyl group of hydroxyacyl-coenzyme A (CoA) substrates. Aeromonas caviae PHA synthase (PhaCAc) is an important biocatalyst for the synthesis of a useful PHA copolymer, poly[(R)-3 ...
Takeharu Tsuge, Tsuge Takeharu
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Enhanced Production of (R)-3-Hydroxybutyrate Oligomers by Coexpression of Molecular Chaperones in Recombinant Escherichia coli Harboring a Polyhydroxyalkanoate Synthase Derived from Bacillus cereus YB-4 [PDF]
Microorganisms, 2022 The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory production of
Saki Goto +4 more
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In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase. [PDF]
Appl Environ Microbiol, 2006 ABSTRACTThis study demonstrated that engineered polyhydroxyalkanoate (PHA) synthases can be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface. The β-galactosidase was fused to the N terminus of the class II PHA synthase fromPseudomonas aeruginosa.
Peters V, Rehm BH.
europepmc +6 more sources
Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis
Current Research in Biotechnology, 2022 Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial petrochemical plastics in certain applications like single-use packaging since they are biodegradable, biocompatible and share similar properties with conventional ...
Min Fey Chek +2 more
exaly +4 more sources
Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaCAR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of
Shuzo Arai +6 more
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Screening Method for Polyhydroxyalkanoate Synthase Mutants Based on Polyester Degree of Polymerization Using High-Performance Liquid Chromatography [PDF]
Microorganisms, 2021 A high-throughput screening method based on the degree of polymerization (DP) of polyhydroxyalkanoate (PHA) was developed using high-performance liquid chromatography (HPLC).
Manami Ishii-Hyakutake +2 more
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Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers [PDF]
FEBS Open BioThe rapid escalation of global plastic consumption, coupled with the environmental impacts of petrochemical polymers, has sparked a surge of interest in bioplastics, particularly those derived from microbial and enzymatic processes.
Giovanni Gallo +4 more
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