Results 41 to 50 of about 2,304 (189)

Active Intermediates of Polyhydroxyalkanoate Synthase from Aeromonas caviae in Polymerization Reaction [PDF]

open access: yesBiomacromolecules, 2012
Polyhydroxyalkanoate (PHA) synthase from Aeromonas caviae FA440 (PhaC(Ac), BAA21815) is one of the most valuable PHA synthase, because of its function to synthesize a practical bioplastic, poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)].
Keiji, Numata   +5 more
openaire   +4 more sources

A Novel Link Between Electron Transport Chain Modulation and mcl-PHA Production in Pseudomonas aeruginosa 5300 Using Azide as a Modulator. [PDF]

open access: yesEnviron Microbiol Rep
The oleic acid and azide inhibited the cytochrome c oxidase activity in Pseudomonas aeruginosa MCC 5300, shifting the electron flux towards bd‐oxidase. This resulted in more NADH consumption to maintain the proton gradient for energy generation. The rapid depletion of NADH triggered high mcl‐PHA accumulation.
Paduvari R, Somashekara D.
europepmc   +2 more sources

Biosynthesis of High-Molecular-Weight Poly(d-lactate)-Containing Block Copolyesters Using Evolved Sequence-Regulating Polyhydroxyalkanoate Synthase PhaC(AR) [PDF]

open access: yes, 2023
Bacterial polyhydroxyalkanoate (PHA) synthase PhaC(AR) is a unique enzyme that can synthesize block copolymers.In thisstudy, poly(d-lactate) (PDLA)-containing block copolymerswere synthesized using PhaC(AR) and its mutated variants.Recombinant ...
Isono, Takuya   +6 more
core   +1 more source

Identification and Analysis of Putative Polyhydroxyalkanoate Synthase (PhaC) in Pseudomonas fluorescens

open access: yesJournal of Microbiology and Biotechnology, 2018
Pseudomonas fluorescens KLR101 was found to be capable of producing polyhydroxyalkanoate (PHA) using various sugars and fatty acids with carbon numbers ranging from 2 to 6. The PHA granules consisted mainly of a poly(3-hydroxybutyrate) homopolymer and/or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. Genomic DNA of P.
Ju Hyoung, Lim   +2 more
openaire   +3 more sources

The alternative sigma factor, σS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida [PDF]

open access: yes, 2008
To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion ...
Raiger Iustman, Laura Judith   +3 more
core   +1 more source

Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.

open access: yesPLoS ONE, 2013
Hexavalent chromium is a serious and widespread environmental pollutant. Although many bacteria have been identified that can transform highly water-soluble and toxic Cr(VI) to insoluble and relatively non-toxic Cr(III), bacterial bioremediation of Cr(VI)
Katherine J Robins   +3 more
doaj   +1 more source

Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material

open access: yesScientific Reports, 2021
The first polyhydroxyalkanoate (PHA) block copolymer poly(2-hydroxybutyrate-b-3-hydroxybutyrate) [P(2HB-b-3HB)] was previously synthesized using engineered Escherichia coli expressing a chimeric PHA synthase PhaCAR with monomer sequence-regulating ...
Yuki Kageyama   +4 more
doaj   +1 more source

SELEÇÃO POLIFÁSICA DE MICRORGANISMOS PRODUTORES DE POLIHIDROXIALCANOATOS

open access: yesQuímica Nova
Polyhydroxyalkanoates (PHAs) are fully biodegradable biopolyesters produced by many prokaryotes and accumulated as insoluble cytoplasmatic inclusions. The detection of these intracellular granule is usually provided using lipofilic azodyes, which are not
Amanda L. S. Silva   +3 more
doaj   +1 more source

RNA-Seq analysis provides insights for understanding photoautotrophic polyhydroxyalkanoate production in recombinant Synechocystis Sp. [PDF]

open access: yesPLoS ONE, 2014
The photosynthetic cyanobacterium, Synechocystis sp. strain 6803, is a potential platform for the production of various chemicals and biofuels. In this study, direct photosynthetic production of a biopolymer, polyhydroxyalkanoate (PHA), in genetically ...
Nyok-Sean Lau   +4 more
doaj   +1 more source

Exploring Class I polyhydroxyalkanoate synthases with broad substrate specificity for polymerization of structurally diverse monomer units

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
Polyhydroxyalkanoate (PHA) synthases (PhaCs) are key enzymes in PHA polymerization. PhaCs with broad substrate specificity are attractive for synthesizing structurally diverse PHAs.
Ramamoorthi M Sivashankari   +7 more
doaj   +1 more source

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