Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics. [PDF]
Angew Chem Int Ed EnglAbstract Polyhydroxyalkanoate (PHA) is a biodegradable polyester that can serve as a promising alternative to petrochemical plastics, which present a serious source of pollution. PHA synthase (PhaC) is a key enzyme responsible for producing a wide variety of PHAs in microorganisms.
Chek MF +5 more
europepmc +4 more sources
Novel polyhydroxyalkanoate copolymers produced in Pseudomonas putida by metagenomic polyhydroxyalkanoate synthases [PDF]
Applied Microbiology and Biotechnology, 2016 Bacterially produced biodegradable polyhydroxyalkanoates (PHAs) with versatile properties can be achieved using different PHA synthases (PhaCs). This work aims to expand the diversity of known PhaCs via functional metagenomics and demonstrates the use of these novel enzymes in PHA production.
Jiujun, Cheng, Trevor C, Charles
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Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate [PDF]
ACS Omega, 2017 Mieko Higuchi-Takeuchi +3 more
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Engineering of Chimeric Class II Polyhydroxyalkanoate Synthases [PDF]
Applied and Environmental Microbiology, 2004 ABSTRACT PHA synthase is a key enzyme involved in the biosynthesis of polyhydroxyalkanoates (PHAs). Using a combinatorial genetic strategy to create unique chimeric class II PHA synthases, we have obtained a number of novel chimeras which display improved catalytic properties.
Nuttawee, Niamsiri +3 more
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In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase. [PDF]
Appl Environ Microbiol, 2006 ABSTRACTThis study demonstrated that engineered polyhydroxyalkanoate (PHA) synthases can be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface. The β-galactosidase was fused to the N terminus of the class II PHA synthase fromPseudomonas aeruginosa.
Peters V, Rehm BH.
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Phasin Proteins Activate Aeromonas caviae Polyhydroxyalkanoate (PHA) Synthase but Not Ralstonia eutropha PHA Synthase [PDF]
Applied and Environmental Microbiology, 2014 ABSTRACT In this study, we performed in vitro and in vivo activity assays of polyhydroxyalkanoate (PHA) synthases (PhaCs) in the presence of phasin proteins (PhaPs), which revealed that PhaPs are activators of PhaC derived from Aeromonas caviae (PhaC
Kazunori Ushimaru +3 more
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Two Polyhydroxyalkanoate Synthases from Distinct Classes from the Aromatic Degrader Cupriavidus pinatubonensis JMP134 Exhibit the Same Substrate Preference. [PDF]
PLoS ONE, 2015 Cupriavidus pinatubonensis JMP134 utilizes a variety of aromatic substrates as sole carbon sources, including meta-nitrophenol (MNP). Two polyhydroxyalkanoate (PHA) synthase genes, phaC1 and phaC2, were annotated and categorized as class I and class II ...
Xuan Jiang, Xi Luo, Ning-Yi Zhou
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Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. [PDF]
Appl Environ Microbiol, 2011 ABSTRACT The synthesis of bacterial polyhydroxyalkanoates (PHA) is very much dependent on the expression and activity of a key enzyme, PHA synthase (PhaC). Many efforts are being pursued to enhance the activity and broaden the substrate specificity of PhaC.
Bhubalan K +7 more
europepmc +6 more sources
Journal of Phycology, 2021 Using Nile Red and BODIPY 493/503 dye‐staining and fluorescence microscopy, twenty cyanobacterial strains, including ten commercially available strains and ten environmental isolates from estuaries, freshwater ponds, and lagoons, were screened for the accumulation of ecologically important and potentially biotechnologically significant carbon storage ...
Karl Hong +4 more
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Microbial Cell Factories, 2023 Background This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions.
Anuyut Yootoum +9 more
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