Results 21 to 30 of about 166,439 (190)

Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate [PDF]

ACS Omega, 2017
Mieko Higuchi-Takeuchi, Yoko Motoda, Takanori Kigawa, Keiji Numata   +3 more
doaj   +2 more sources

Advances in Polyhydroxyalkanoate (PHA) Production, Volume 3 [PDF]

, 2022
Nowadays, we are witnessing highly dynamic research activities related to the intriguing field of biodegradable materials with plastic-like properties. These activities are currently intensified by a strengthened public awareness of prevailing ecological

core   +1 more source

Engineering of Chimeric Class II Polyhydroxyalkanoate Synthases [PDF]

Applied and Environmental Microbiology, 2004
ABSTRACT PHA synthase is a key enzyme involved in the biosynthesis of polyhydroxyalkanoates (PHAs). Using a combinatorial genetic strategy to create unique chimeric class II PHA synthases, we have obtained a number of novel chimeras which display improved catalytic properties.
Nuttawee, Niamsiri, Soazig C, Delamarre, Young-Rok, Kim, Carl A, Batt   +3 more
openaire   +2 more sources

Two Polyhydroxyalkanoate Synthases from Distinct Classes from the Aromatic Degrader Cupriavidus pinatubonensis JMP134 Exhibit the Same Substrate Preference. [PDF]

PLoS ONE, 2015
Cupriavidus pinatubonensis JMP134 utilizes a variety of aromatic substrates as sole carbon sources, including meta-nitrophenol (MNP). Two polyhydroxyalkanoate (PHA) synthase genes, phaC1 and phaC2, were annotated and categorized as class I and class II ...
Xuan Jiang, Xi Luo, Ning-Yi Zhou
doaj   +1 more source

Characterization of newly isolated thermotolerant bacterium Cupriavidus sp. CB15 from composting and its ability to produce polyhydroxyalkanoate from glycerol

Microbial Cell Factories, 2023
Background This study aimed to isolate a novel thermotolerant bacterium that is capable of synthesizing polyhydroxyalkanoate from glycerol under high temperature conditions.
Anuyut Yootoum, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Churairat Moukamnerd, Thanongsak Chaiyaso, Chayakorn Pumas, Nuttapol Tanadchangsaeng, Masanori Watanabe, Toshiaki Fukui, Chayatip Insomphun   +9 more
doaj   +1 more source

Screening and characterization of polyhydroxyalkanoate granules, and phylogenetic analysis of polyhydroxyalkanoate synthase gene PhaC in cyanobacteria

Journal of Phycology, 2021
Using Nile Red and BODIPY 493/503 dye‐staining and fluorescence microscopy, twenty cyanobacterial strains, including ten commercially available strains and ten environmental isolates from estuaries, freshwater ponds, and lagoons, were screened for the accumulation of ecologically important and potentially biotechnologically significant carbon storage ...
Karl Hong, Joris Beld, Tony D. Davis, Michael D. Burkart, Brian Palenik   +4 more
openaire   +3 more sources

Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics. [PDF]

Angew Chem Int Ed Engl
Polyhydroxyalkanoate (PHA) is a biodegradable polyester that can serve as a promising alternative to petrochemical plastics, which present a serious source of pollution. PHA synthase (PhaC) is a key enzyme responsible for producing a wide variety of PHAs
Chek MF, Kim SY, Mori T, Matsumoto K, Sato S, Hakoshima T.   +5 more
europepmc   +3 more sources

Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. [PDF]

Appl Environ Microbiol, 2011
ABSTRACT The synthesis of bacterial polyhydroxyalkanoates (PHA) is very much dependent on the expression and activity of a key enzyme, PHA synthase (PhaC). Many efforts are being pursued to enhance the activity and broaden the substrate specificity of PhaC.
Bhubalan K, Chuah JA, Shozui F, Brigham CJ, Taguchi S, Sinskey AJ, Rha C, Sudesh K.   +7 more
europepmc   +6 more sources

Combination of N149S and D171G mutations inAeromonas caviaepolyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis [PDF]

FEMS Microbiology Letters, 2007
Aeromonas caviae polyhydroxyalkanoate synthase (PhaC(Ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)]. In a previous study, two PhaC(Ac) mutants that have a single amino acid substitution of either asparagine 149 ...
Takeharu Tsuge, Shinko Watanabe, Daisuke Shimada, Hideki Abe, Yoshiharu Doi, Seiichi Taguchi   +5 more
openaire   +2 more sources

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110 [PDF]

Journal of Bacteriology, 2013
Bradyrhizobium japonicum USDA 110 has five polyhydroxyalkanoate (PHA) synthases (PhaC) annotated in its genome: bll4360 (phaC1), bll6073 (phaC2), blr3732 (phaC3), blr2885 (phaC4), and bll4548 (phaC5). All these proteins possess the catalytic triad and conserved amino acid residues of polyester synthases and are distributed into four different PhaC ...
Quelas, Juan Ignacio, Mongiardini, Elías Javier, Pérez Giménez, Julieta, Parisi, Gustavo Daniel, Lodeiro, Aníbal Roberto   +4 more
openaire   +4 more sources