Heterologous expression and characterization of polyhydroxyalkanoate synthase genes from haloarchaeal strains in Haloferax mediterranei [PDF]
Frontiers in MicrobiologyIntroductionPolyhydroxyalkanoates (PHAs) are promising materials for building a sustainable society due to their excellent biodegradability. Some haloarchaea, which require high salt concentrations for growth, possess class III PHA synthases (PhaECs) and
Keisuke Wada +3 more
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Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon Haloferax mediterranei: Key Enzyme of Biodegradable Plastic Synthesis [PDF]
BioengineeringThe biosynthesis of polyhydroxyalkanoate (PHA) biopolymer is highly dependent on the activity of a key enzyme, PHA synthase (PhaC). The halophilic archaeon Haloferax mediterranei can accumulate large amounts of PHAs from different carbon sources under ...
Diya Alsafadi +2 more
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Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility
, 2022 Recombinant E. coli producing intein-cleavable polyhydroxyalkanoate synthase fusions mediates the intracellular formation of polyhydroxyalkanoate (PHA) particles densely coated with intein-cleavable target protein fusion. These PHA particles can be efficiently purified from lysed cells.
Chen, S, Rehm, B
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Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR [PDF]
Microbial Cell Factories, 2022 Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence.
Keigo Satoh +6 more
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Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. [PDF]
Appl Environ Microbiol, 2011 ABSTRACT The synthesis of bacterial polyhydroxyalkanoates (PHA) is very much dependent on the expression and activity of a key enzyme, PHA synthase (PhaC). Many efforts are being pursued to enhance the activity and broaden the substrate specificity of PhaC.
Bhubalan K +7 more
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Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria [PDF]
Microbial Cell FactoriesPolyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential.
Abdiqani Ibrahim Osman +3 more
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Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics [PDF]
Scientific Reports, 2017 Polyhydroxyalkanoate (PHA) is a promising candidate for use as an alternative bioplastic to replace petroleum-based plastics. Our understanding of PHA synthase PhaC is poor due to the paucity of available three-dimensional structural information. Here we
Min Fey Chek +6 more
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High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes. [PDF]
PLoS ONE, 2014 Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species.
Mariela V Catone +5 more
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Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones. [PDF]
Appl Environ Microbiol, 2013 ABSTRACT The type I polyhydroxyalkanoate synthase from Cupriavidus necator was heterologously expressed in Escherichia coli with simultaneous overexpression of chaperone proteins.
Thomson NM +7 more
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PHA synthase variant design using a conditional variational autoencoder. [PDF]
PLoS Computational BiologyPolyhydroxyalkanoate (PHA) synthases are a group of complex, dimeric enzymes which catalyze polymerization of R-hydroxyacids into PHAs. PHA properties depend on their monomer composition but enzymes found in nature have limited specificities to certain R-
Tuula Tenkanen +4 more
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