Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis
Current Research in Biotechnology, 2022 Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial petrochemical plastics in certain applications like single-use packaging since they are biodegradable, biocompatible and share similar properties with conventional ...
Javier Linares-Pastén +2 more
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Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria [PDF]
Microbial Cell FactoriesPolyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential.
Abdiqani Ibrahim Osman +3 more
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In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthase. [PDF]
Appl Environ Microbiol, 2006 ABSTRACTThis study demonstrated that engineered polyhydroxyalkanoate (PHA) synthases can be employed as molecular tools to covalently immobilize enzymes at the PHA granule surface. The β-galactosidase was fused to the N terminus of the class II PHA synthase fromPseudomonas aeruginosa.
Peters V, Rehm BH.
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Frontiers in Bioengineering and Biotechnology, 2022 Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1Ps6-19) was commonly utilized in the de novo biosynthesis of PLA from biomass.
Jagroop Pandhal, Huibin Zou
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Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility
, 2022 Recombinant E. coli producing intein-cleavable polyhydroxyalkanoate synthase fusions mediates the intracellular formation of polyhydroxyalkanoate (PHA) particles densely coated with intein-cleavable target protein fusion. These PHA particles can be efficiently purified from lysed cells.
Chen, S, Rehm, B
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Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR [PDF]
Microbial Cell Factories, 2022 Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence.
Keigo Satoh +6 more
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High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes. [PDF]
PLoS ONE, 2014 Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species.
Mariela V Catone +5 more
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PHA synthase variant design using a conditional variational autoencoder. [PDF]
PLoS Computational BiologyPolyhydroxyalkanoate (PHA) synthases are a group of complex, dimeric enzymes which catalyze polymerization of R-hydroxyacids into PHAs. PHA properties depend on their monomer composition but enzymes found in nature have limited specificities to certain R-
Tuula Tenkanen +4 more
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Applied Microbiology and Biotechnology, 2015 The polyhydroxyalkanoate (PHA) synthase catalyzes the synthesis of PHA and remains attached to the hydrophobic PHA inclusions it creates. Although this feature is actively exploited to generate functionalized biobeads via protein engineering, little is known about the structure of the PHA synthase.
Hooks, David O, Rehm, Bernd HA
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Novel polyhydroxyalkanoate copolymers produced in Pseudomonas putida by metagenomic polyhydroxyalkanoate synthases [PDF]
Applied Microbiology and Biotechnology, 2016 Bacterially produced biodegradable polyhydroxyalkanoates (PHAs) with versatile properties can be achieved using different PHA synthases (PhaCs). This work aims to expand the diversity of known PhaCs via functional metagenomics and demonstrates the use of these novel enzymes in PHA production.
Jiujun, Cheng, Trevor C, Charles
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