Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis
Current Research in Biotechnology, 2022 Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial petrochemical plastics in certain applications like single-use packaging since they are biodegradable, biocompatible and share similar properties with conventional ...
Soon Zher Neoh +6 more
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Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR [PDF]
Microbial Cell Factories, 2022 Background Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence.
Keigo Satoh +6 more
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Class I Polyhydroxyalkanoate (PHA) Synthase Increased Polylactic Acid Production in Engineered Escherichia Coli [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Polylactic acid (PLA), a homopolymer of lactic acid (LA), is a bio-derived, biocompatible, and biodegradable polyester. The evolved class II PHA synthase (PhaC1Ps6-19) was commonly utilized in the de novo biosynthesis of PLA from biomass.
Mengxun Shi +8 more
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Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110 [PDF]
Journal of Bacteriology, 2013 Bradyrhizobium japonicum USDA 110 has five polyhydroxyalkanoate (PHA) synthases (PhaC) annotated in its genome: bll4360 (phaC1), bll6073 (phaC2), blr3732 (phaC3), blr2885 (phaC4), and bll4548 (phaC5).
Lodeiro, Anibal +4 more
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Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria [PDF]
Microbial Cell FactoriesPolyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential.
Abdiqani Ibrahim Osman +3 more
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Production of functionalized polyhydroxyalkanoates by genetically modified
Microbial Cell Factories, 2010 Background Methylotrophic (methanol-utilizing) bacteria offer great potential as cell factories in the production of numerous products from biomass-derived methanol. Bio-methanol is essentially a non-food substrate, an advantage over sugar-utilizing cell
Miguez Carlos B +5 more
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PHA synthase variant design using a conditional variational autoencoder. [PDF]
PLoS Computational BiologyPolyhydroxyalkanoate (PHA) synthases are a group of complex, dimeric enzymes which catalyze polymerization of R-hydroxyacids into PHAs. PHA properties depend on their monomer composition but enzymes found in nature have limited specificities to certain R-
Tuula Tenkanen +4 more
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High polyhydroxybutyrate production in Pseudomonas extremaustralis is associated with differential expression of horizontally acquired and core genome polyhydroxyalkanoate synthase genes. [PDF]
PLoS ONE, 2014 Pseudomonas extremaustralis produces mainly polyhydroxybutyrate (PHB), a short chain length polyhydroxyalkanoate (sclPHA) infrequently found in Pseudomonas species.
Mariela V Catone +5 more
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Phasins PhbP2 and PhbP3 Are Involved in the Depolymerization of Polyhydroxybutyrate in Azotobacter vinelandii. [PDF]
J Basic MicrobiolABSTRACT Polyhydroxybutyrate (PHB) is a natural polyester synthesized and stored intracellularly as cytoplasmic granules, serving as a carbon and energy reserve in certain bacteria. These granules are surrounded by a variety of proteins, including those directly involved in PHB metabolism—both in its synthesis and degradation—as well as a group of non ...
Ruiz-Escobedo J +8 more
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A Novel Link Between Electron Transport Chain Modulation and mcl-PHA Production in Pseudomonas aeruginosa 5300 Using Azide as a Modulator. [PDF]
Environ Microbiol RepThe oleic acid and azide inhibited the cytochrome c oxidase activity in Pseudomonas aeruginosa MCC 5300, shifting the electron flux towards bd‐oxidase. This resulted in more NADH consumption to maintain the proton gradient for energy generation. The rapid depletion of NADH triggered high mcl‐PHA accumulation.
Paduvari R, Somashekara D.
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