Results 31 to 40 of about 2,304 (189)

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110 [PDF]

Journal of Bacteriology, 2013
Bradyrhizobium japonicum USDA 110 has five polyhydroxyalkanoate (PHA) synthases (PhaC) annotated in its genome: bll4360 (phaC1), bll6073 (phaC2), blr3732 (phaC3), blr2885 (phaC4), and bll4548 (phaC5). All these proteins possess the catalytic triad and conserved amino acid residues of polyester synthases and are distributed into four different PhaC ...
Quelas, Juan Ignacio, Mongiardini, Elías Javier, Pérez Giménez, Julieta, Parisi, Gustavo Daniel, Lodeiro, Aníbal Roberto   +4 more
openaire   +4 more sources

Inhibitors of Polyhydroxyalkanoate (PHA) Synthases: Synthesis, Molecular Docking, and Implications [PDF]

ChemBioChem, 2014
AbstractPolyhydroxyalkanoate (PHA) synthases (PhaCs) catalyze the formation of biodegradable PHAs that are considered to be ideal alternatives to non‐biodegradable synthetic plastics. However, study of PhaCs has been challenging because the rate of PHA chain elongation is much faster than that of initiation.
Wei, Zhang, Chao, Chen, Ruikai, Cao, Leila, Maurmann, Ping, Li   +4 more
openaire   +2 more sources

Characterization of the Polyhydroxyalkanoate Synthase Gene Locus of Rhodobacter Sphaeroides [PDF]

Biotechnology Letters, 1993
The Rhodobacter sphaeroides polyhydroxyalkanoate synthase structural gene (phaCRs) was identified as a 1,806-bp open reading frame. It encoded a PHA synthase of 601 amino acids with a relative molecular mass of 66,742, which exhibited 25.9 to 39.0 % amino acid identity with PHA synthases from other bacteria.
Eilert Hustede, Alexander Steinbüchel
  +6 more sources

Optimization of Culture Conditions for Secretory Production of 3-Hydroxybutyrate Oligomers Using Recombinant Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2022
Poly(3-hydroxybutyrate) [P(3HB)] is the most representative polyhydroxyalkanoate (PHA), which is a storage polyester for prokaryotic cells. P(3HB)-producing recombinant Escherichia coli secretes diethylene glycol (DEG)-terminated 3HB oligomers (3HBO-DEG)
Tetsuo Sakurai, Tetsuo Sakurai, Shoji Mizuno, Shoji Mizuno, Yuki Miyahara, Yuki Miyahara, Ayaka Hiroe, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge, Takeharu Tsuge   +10 more
doaj   +1 more source

PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces

Frontiers in Microbiology, 2021
The Mycobacteria are a genus of Actinobacteria that include human pathogens such as Mycobacterium tuberculosis (TB). Active TB disease can spread by airborne transmission to healthcare workers and to their community.
Courtney G. Davies, Kerri Reilly, Eric Altermann, Eric Altermann, Heather L. Hendrickson, Heather L. Hendrickson   +5 more
doaj   +1 more source

Polyhydroyxalkanoate Synthase Fusions as a Strategy for Oriented Enzyme Immobilisation

Molecules, 2014
Polyhydroxyalkanoate (PHA) is a carbon storage polymer produced by certain bacteria in unbalanced nutrient conditions. The PHA forms spherical inclusions surrounded by granule associate proteins including the PHA synthase (PhaC).
David O. Hooks, Mark Venning-Slater, Jinping Du, Bernd H. A. Rehm   +3 more
doaj   +1 more source

One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains

Nature Communications, 2018
Fermentative production of aromatic polyesters from glucose has been unsuccessful. Here, the authors achieve the objective by one-step fermentation of metabolically engineered E. coli expressing a isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase and
Jung Eun Yang, Si Jae Park, Won Jun Kim, Hyeong Jun Kim, Bumjoon J. Kim, Hyuk Lee, Jihoon Shin, Sang Yup Lee   +7 more
doaj   +1 more source

Phasins PhbP2 and PhbP3 Are Involved in the Depolymerization of Polyhydroxybutyrate in Azotobacter vinelandii. [PDF]

J Basic Microbiol
ABSTRACT Polyhydroxybutyrate (PHB) is a natural polyester synthesized and stored intracellularly as cytoplasmic granules, serving as a carbon and energy reserve in certain bacteria. These granules are surrounded by a variety of proteins, including those directly involved in PHB metabolism—both in its synthesis and degradation—as well as a group of non ...
Ruiz-Escobedo J, Guzmán J, Espín G, Barrientos-Millán T, Moyao-Mejía A, Peña C, Moreno S, Meneses-Romero EP, Segura D.   +8 more
europepmc   +2 more sources

Real-time NMR analysis of polyhydroxyalkanoate synthase reaction that synthesizes block copolymer comprising glycolate and 3-hydroxybutyrate

, 2023
The sequence-regulating polyhydroxyalkanoate (PHA) synthase PhaCAR spontaneously synthesizes the homo-random block copolymer, poly[3-hydroxybutyrate (3HB)]-b-poly[glycolate (GL)-ran-3HB]. In this study, a real-time in vitro chasing system was established
Ayaka Kajikawa, Sayaka Sakakibara, Hiroya Tomita, Kajikawa, Ayaka, Kumeta, Hiroyuki, Hiroyuki Kumeta, Matsumoto, Ken'ichiro, Kengo Yanagawa, Ken'ichiro Matsumoto, Yanagawa, Kengo, Sakakibara, Sayaka, Tomita, Hiroya   +11 more
core   +1 more source

Polyhydroxyalkanoate Producing Potential of Saccharomyces cerevisiae

Fountain Journal of Natural and Applied Sciences (FUJNAS), 2018
Polyhydroxyalkanoates (PHAs) are inclusion bodies accumulated by some microorganisms as reserve material under unbalanced growth condition such as limited oxygen, nitrogen, phosphorous or sulphur and excess carbon source.
S. O. Jimoh, F. O. Akinwande, O. A. Ayinde, T. Y. Senbadejo   +3 more
doaj   +3 more sources