Results 31 to 40 of about 4,020 (197)

Inhibitors of Polyhydroxyalkanoate (PHA) Synthases: Synthesis, Molecular Docking, and Implications [PDF]

ChemBioChem, 2014
AbstractPolyhydroxyalkanoate (PHA) synthases (PhaCs) catalyze the formation of biodegradable PHAs that are considered to be ideal alternatives to non‐biodegradable synthetic plastics. However, study of PhaCs has been challenging because the rate of PHA chain elongation is much faster than that of initiation.
Wei, Zhang, Chao, Chen, Ruikai, Cao, Leila, Maurmann, Ping, Li   +4 more
openaire   +2 more sources

Combination of N149S and D171G mutations inAeromonas caviaepolyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis [PDF]

FEMS Microbiology Letters, 2007
Aeromonas caviae polyhydroxyalkanoate synthase (PhaC(Ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)]. In a previous study, two PhaC(Ac) mutants that have a single amino acid substitution of either asparagine 149 ...
Takeharu Tsuge, Shinko Watanabe, Daisuke Shimada, Hideki Abe, Yoshiharu Doi, Seiichi Taguchi   +5 more
openaire   +2 more sources

Engineering Bacillus megaterium for production of functional intracellular materials [PDF]

, 2017
Background: Over the last 10-15 years, a technology has been developed to engineer bacterial polyhydroxybutyrate (PHB) inclusions as functionalized beads, for applications such as vaccines, diagnostics and enzyme immobilization. This has been achieved by
Grage, K, McDermott, P, Rehm, BHA
core   +3 more sources

Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility

, 2022
Recombinant E. coli producing intein-cleavable polyhydroxyalkanoate synthase fusions mediates the intracellular formation of polyhydroxyalkanoate (PHA) particles densely coated with intein-cleavable target protein fusion. These PHA particles can be efficiently purified from lysed cells.
Chen, S, Rehm, B
openaire   +3 more sources

Optimization of Culture Conditions for Secretory Production of 3-Hydroxybutyrate Oligomers Using Recombinant Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2022
Poly(3-hydroxybutyrate) [P(3HB)] is the most representative polyhydroxyalkanoate (PHA), which is a storage polyester for prokaryotic cells. P(3HB)-producing recombinant Escherichia coli secretes diethylene glycol (DEG)-terminated 3HB oligomers (3HBO-DEG)
Tetsuo Sakurai, Tetsuo Sakurai, Shoji Mizuno, Shoji Mizuno, Yuki Miyahara, Yuki Miyahara, Ayaka Hiroe, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge, Takeharu Tsuge   +10 more
doaj   +1 more source

PLAN-M; Mycobacteriophage Endolysins Fused to Biodegradable Nanobeads Mitigate Mycobacterial Growth in Liquid and on Surfaces

Frontiers in Microbiology, 2021
The Mycobacteria are a genus of Actinobacteria that include human pathogens such as Mycobacterium tuberculosis (TB). Active TB disease can spread by airborne transmission to healthcare workers and to their community.
Courtney G. Davies, Kerri Reilly, Eric Altermann, Eric Altermann, Heather L. Hendrickson, Heather L. Hendrickson   +5 more
doaj   +1 more source

Polyhydroyxalkanoate Synthase Fusions as a Strategy for Oriented Enzyme Immobilisation

Molecules, 2014
Polyhydroxyalkanoate (PHA) is a carbon storage polymer produced by certain bacteria in unbalanced nutrient conditions. The PHA forms spherical inclusions surrounded by granule associate proteins including the PHA synthase (PhaC).
David O. Hooks, Mark Venning-Slater, Jinping Du, Bernd H. A. Rehm   +3 more
doaj   +1 more source

The effect of temperature during culture enrichment on methanotrophic polyhydroxyalkanoate production [PDF]

, 2019
Producción CientíficaClimate change and plastic pollution are likely the most relevant environmental problems of the 21st Century. Thus, one of the most promising solutions to remedy both environmental problems simultaneously is the bioconversion of ...
Bordel Velasco, Sergio, Cantera Ruiz de Pellón, Sara, García Encina, Pedro Antonio, Muñoz Torre, Raúl, Pérez Fernández, Rebeca   +4 more
core   +2 more sources

One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains

Nature Communications, 2018
Fermentative production of aromatic polyesters from glucose has been unsuccessful. Here, the authors achieve the objective by one-step fermentation of metabolically engineered E. coli expressing a isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase and
Jung Eun Yang, Si Jae Park, Won Jun Kim, Hyeong Jun Kim, Bumjoon J. Kim, Hyuk Lee, Jihoon Shin, Sang Yup Lee   +7 more
doaj   +1 more source

Poly(3-hydroxybutyrate) hyperproduction by a global nitrogen regulator NtrB mutant strain of Paracoccus denitrificans PD1222 [PDF]

, 2017
Paracoccus denitrificans PD1222 accumulates short-length polyhydroxyalkanoates, poly(3-hydroxybutyrate), under nitrogen-deficient conditions. Polyhydroxybutyrate metabolism requires the 3-ketoacyl-CoA thiolase PhaA, the acetoacetyl-CoA dehydrogenase ...
Alfonso Olaya-Abril, Andrew J Gates, Ashby, Cawse, Chanasit, Chen, Chen, Chen, Conrado Moreno-Vivián, David J Richardson, Fukui, Galán, García, Gates, Hang, Harms, Hoffmann, Huisman, Isabel Manso, Jendrossek, Jendrossek, Kalaiyezhini, Kojima, Koller, Kourmentza, Kourmentza, Lee, Liebergesell, Luque-Almagro, Luque-Almagro, Madison, Maehara, Manso, María Dolores Roldán, Matsusaki, McCool, Muhammadi, Prieto, Pötter, Rehm, Richardson, Satoh, Schubert, Shakir, Singh, Sun, Tortajada, Vadamme, Víctor M Luque-Almagro, Zuriani   +49 more
core   +2 more sources