Results 81 to 90 of about 4,379 (172)

A model for carbohydrate metabolism in the diatom Phaeodactylum tricornutum deduced from comparative whole genome analysis.

PLoS ONE, 2008
BackgroundDiatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids.Methodology ...
Peter G Kroth, Anthony Chiovitti, Ansgar Gruber, Veronique Martin-Jezequel, Thomas Mock, Micaela Schnitzler Parker, Michele S Stanley, Aaron Kaplan, Lise Caron, Till Weber, Uma Maheswari, E Virginia Armbrust, Chris Bowler   +12 more
doaj   +1 more source

Iron Deficiency Induces a Partial Inhibition of the Photosynthetic Electron Transport and a High Sensitivity to Light in the Diatom Phaeodactylum tricornutum

Frontiers in Plant Science, 2016
Iron limitation is the major factor controlling phytoplankton growth in vast regions of the contemporary oceans. In this study, a combination of thermoluminescence, chlorophyll fluorescence and P700 absorbance measurements have been used to elucidate the
Mercedes Roncel, Antonio Alfonso González-Rodríguez, Belén Naranjo, Pilar Bernal-Bayard, Anna Marika Lindahl, Manuel Hervás, José Antonio Navarro, José María Ortega   +7 more
doaj   +1 more source

Advances in Phaeodactylum tricornutum nuclear engineering

, 2023
The marine diatom Phaeodactylum tricornutum has the potential to become an excellent platform for the sustainable production of valuable compounds and pharmaceuticals, but currently large-scale engineering of this organism remains a challenge due factors like inefficient genetic transformation protocols and a lack of accurate genomic data.
openaire   +1 more source

GG1 - sgRNA cloning for Phaeodactylum tricornutum v1 [PDF]

, 2019
The CRISPR-Cas9 gene mutagenesis system was adapted for the marine diatom Phaeodactylum tricornutum (CCAP-1055/1) (Figure 1). Here, Cas9 and sgRNA(s) were delivered to Phaeodactylum by bacterial-conjugation transformation on an episome, or artificial chromosome, that is stably maintained and replicated independently from and with the Phaeodactylum ...
Mark Moosburner, Andrew E Allen
openaire   +1 more source

The Role of Malic Enzyme on Promoting Total Lipid and Fatty Acid Production in Phaeodactylum tricornutum

Frontiers in Plant Science, 2018
To verify the function of malic enzyme (ME1), the ME1 gene was endogenously overexpressed in Phaeodactylum tricornutum. Overexpression of ME1 increased neutral and total lipid content and significantly increased saturated fatty acids (SFAs) and ...
Bao-Hua Zhu, Rui-Hao Zhang, Na-Na Lv, Guan-Pin Yang, Yi-Sheng Wang, Ke-Hou Pan, Ke-Hou Pan   +6 more
doaj   +1 more source

A Linear Programming Approach for Modeling and Simulation of Growth and Lipid Accumulation of Phaeodactylum tricornutum

Energies, 2013
The unicellular microalga Phaeodactylum tricornutum exhibits the ability to accumulate triacylglycerols to a high specific content when nutrients are limited in the culture medium. Therefore, the organism is a promising candidate for biodiesel production.
Clemens Posten, Robert Dillschneider
doaj   +1 more source

Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different ∆6- and ∆5-desaturases co-expressed with LPCAT from the marine diatom Phaeodactylum tricornutum

Scientific Reports
Continuous research on obtaining an even more efficient production of very long-chain polyunsaturated fatty acids (VLC-PUFAs) in plants remains one of the main challenges of scientists working on plant lipids.
Sylwia Klińska-Bąchor, Kamil Demski, Yangmin Gong, Antoni Banaś   +3 more
doaj   +1 more source

Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics

Scientific Reports
The marine diatom Phaeodactylum tricornutum is currently used for various industrial applications, including the pharmaceutical industry as a cost-effective cell biofactory to produce Biologics. Recent studies demonstrated that P. tricornutum can produce
Isabelle Boulogne, Charlotte Toustou, Muriel Bardor   +2 more
doaj   +1 more source

Comparative RNA-Seq of Ten Phaeodactylum tricornutum Accessions: Unravelling Criteria for Robust Strain Selection from a Bioproduction Point of View

Marine Drugs
The production of biologics in mammalian cells is hindered by some limitations including high production costs, prompting the exploration of other alternative expression systems that are cheaper and sustainable like microalgae.
Charlotte Toustou, Isabelle Boulogne, Anne-Alicia Gonzalez, Muriel Bardor   +3 more
doaj   +1 more source

Genome scale metabolic modelling of Phaeodactylum tricornutum

, 2017
Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable and diverse marine phytoplankton. Their ability to synthesise lipid as a storage compound (20%-50% dry cell weight) makes them a potential sources of biofuel and high-value commodities such as ω fatty acids.
openaire   +1 more source