Results 171 to 180 of about 169,330 (288)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Genomic epidemiology and antimicrobial resistance of non-typhoidal Salmonella from retail meats in Beijing, China: implications for foodborne transmission and phage susceptibility. [PDF]

JAC Antimicrob Resist
Chen A, Li H, Wang Y, Hu X, Li T, Sun L, Zuo Y, Huang P, Xi Y, Wang Y, Liu Y, Zhang Y, Wang S.   +12 more
europepmc   +1 more source

Specific peptide enrichment strategies for neuropsychiatric disorders: From material design to diagnostic applications

Interdisciplinary Medicine, EarlyView.
This review outlines specific peptide enrichment strategies, including metal, magnetic oxides, functional polymers, and antibody‐based approaches, and their mechanisms, highlighting applications in biomarker discovery for major neurological and psychiatric disorders.
Xue Zhang, Yejiao Shi, Minghui Guo, Yang Huang, Yirong Wang, Leping Huang, Helena S. Azevedo, Honggang Hu, Xin Dong   +8 more
wiley   +1 more source

From colonization to infection: Genomic evolution of Clostridioides difficile pathogenesis. [PDF]

Cell Rep
Khanna S, Yang Z, Dubberke ER, Dantas G.
europepmc   +1 more source

Production of recombinant human granulocyte‐macrophage colony‐stimulating factor in thermoinducible E. coli: Tuning inclusion body architecture and protein yield via dissolved oxygen control

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Recombinant protein expression in Escherichia coli frequently results in the formation of inclusion bodies (IBs), which provide high protein accumulation but often require additional steps to recover functional protein. Although culture conditions strongly influence IB physicochemical and structural characteristics, the impact of ...
Ximena Gómez‐Gil, Norma A Valdez‐Cruz, Sara Restrepo‐Pineda, Nuria Sánchez‐Puig, Rafael Vázquez‐Duhalt, Rubén D Cadena‐Nava, Néstor O Pérez, Mauricio A Trujillo‐Roldán   +7 more
wiley   +1 more source

Actinobacillus pleuropneumoniae - today's perspective on advances and challenges (2017- 2026). [PDF]

BMC Vet Res
Hennig-Pauka I, Strutzberg-Minder K, Langford PR.   +2 more
europepmc   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Use of a Bacteriophage Lysin to Control Axillary Malodor

JEADV Clinical Practice, EarlyView.
ABSTRACT Background Axillary malodor is primarily caused by bacteria, particularly Staphylococcus hominis, which metabolize apocrine secretions into volatile compounds. Traditional deodorants contain antiseptics that can disrupt the skin microbiome and cause irritation. Alternative methods of odor control are being sought but so far without success. We
Maurice A. M. van Steensel, Fabian Hia, Mei Zhen Beh, Boon Chong Goh   +3 more
wiley   +1 more source

Seasonal Occurrence, Population Diversity, and Mobilome Features of Environmental <i>Vibrio parahaemolyticus</i> in Coastal and Estuarine Waters of Haiyan, Zhejiang, China. [PDF]

Microorganisms
Xu J, He Y, He P.
europepmc   +1 more source

Isolation and characterization of a novel <i>Staphylococcus</i> phage SPD: genomic insights and its anti-biofilm efficacy against methicillin-resistant <i>Staphylococcus aureus</i>. [PDF]

Front Cell Infect Microbiol
Zheng X, Ma L, Li Y, Liu B, Zhang W, Su X, Liu Y.   +6 more
europepmc   +1 more source