Results 141 to 150 of about 116,257 (347)

Dynamic Binder Exchange Improves Protein Labeling Efficiency in DNA‐PAINT up to 15‐Fold

Angewandte Chemie International Edition, EarlyView.
Dynamic Binder Exchange (DyBE) leverages transient binder–target interactions to enhance labeling efficiency by up to 15‐fold in super‐resolution microscopy. DyBE maps nanoscale receptor tyrosine kinase (RTK) assemblies, including HER2 homodimers and EGFR–HER2 heterodimers, advancing single‐protein spatial proteomics.
Clemens Steinek, Isabelle Pachmayr, Sebastian Strauss, Monique Honsa, Jisoo Kwon, Ralf Jungmann   +5 more
wiley   +1 more source

Heat Stress and Gut Microbiome Dynamics in Poultry: Interplay, Consequences, and Mitigation Strategies

Animal Research and One Health, EarlyView.
Heat stress disrupts gut microbial balance in poultry, impairing nutrient absorption and immunity. This review outlines the interplay between thermal stress and microbiome dynamics and discusses integrative mitigation strategies, probiotics, phytogenics, cooling systems, and genetic adaptation to enhance poultry resilience.
O. E. Oke, A. I. Oni, O. A. Akosile, K. M. Oliyide, C. A. Ishola, M. O. Logunleko, O. A. Jimoh, C. P. Njoku, L. O. Fasasi, V. A. Uyanga   +9 more
wiley   +1 more source

Architectonics of Phage-Liposome Nanowebs as Optimized Photosensitizer Vehicles for Photodynamic Cancer Therapy [PDF]

, 2010
Sreeram Kalarical Janardhanan, Shoba Narayan, Gopal Abbineni, Andrew Hayhurst, Chuanbin Mao   +4 more
openalex   +1 more source

Engineered Stable, Antibiotic‐Free, High‐Level Protein Expression in the Probiotic Chassis Escherichia coli Nissle 1917

Biotechnology and Bioengineering, EarlyView.
The probiotic E. coli Nissle 1917 was engineered to enable stable and antibiotic free protein expression platforms using native plasmids. Further modification aids in the production of high‐level protein expression for antimicrobial secretion whose activity is comparable to industry standard E. coli BL21.
Halimatun Sakdiah Zainuddin, Sanjeeva Kumar Murali, Thomas J. Mansell   +2 more
wiley   +1 more source

High Yield Branched Puromycin Linker Design Enables Efficient cDNA Display and Chemical Modification of Peptides

Biotechnology and Bioengineering, EarlyView.
A novel puromycin linker for cDNA display was synthesized via SPAAC reaction. Its functionality was confirmed by recovering intact DNA after selection against EpCAM. The system also enabled the verification of bicyclic peptides during display, demonstrating its utility for constructing and screening structurally complex peptide libraries.
Simon Schneider, Melanie Boll, Matthias Eder, Ann‐Christin Eder   +3 more
wiley   +1 more source

Targeted treatment of chondrosarcoma with a bacteriophage-based particle delivering a secreted tumor necrosis factor-related apoptosis-inducing ligand

Molecular Therapy: Oncology
Chondrosarcoma (CS) is a malignant cartilage-forming bone tumor that is inherently resistant to chemotherapy and radiotherapy, leaving surgery as the only treatment option.
Aitthiphon Chongchai, Kaoutar Bentayebi, Grace Chu, Wenqing Yan, Sajee Waramit, Thanyaluck Phitak, Prachya Kongtawelert, Peraphan Pothacharoen, Keittisak Suwan, Amin Hajitou   +9 more
doaj   +1 more source

Influence of environmental variables in the efficiency of phage therapy in aquaculture [PDF]

, 2014
Yolanda J. Silva, Liliana Costa, Carla Pereira, Ângela Cunha, Ricardo Calado, Newton C. M. Gomes, Adelaide Almeida   +6 more
openalex   +1 more source

Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions

Biotechnology Progress, EarlyView.
Abstract As adeno‐associated viral vectors (AAV) continue to advance through the clinical pipeline, effective downstream purification strategies must be developed to ensure bulk drug purity and safety. AAV are produced within mammalian cells, bringing forth risks associated with viral contamination.
Akshay S. Chaubal, Asingsa W. Arachchige, Annabelle J. Zahn, S. Ranil Wickramasinghe, Xianghong Qian, Andrew L. Zydney   +5 more
wiley   +1 more source

Phage Therapy: Concept to Cure [PDF]

, 2012
Eric C. Keen
openalex   +1 more source

Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications

Chemistry – A European Journal, EarlyView.
Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley   +1 more source