Results 31 to 40 of about 22,867 (253)

Campylobacter jejuni Developed the Resistance to Bacteriophage CP39 by Phase Variable Expression of 06875 Encoding the CGPTase

Viruses, 2022
Bacteriophage (phage) is regarded as an antimicrobial alternative for Campylobacter in food production. However, the development of phage resistance to the host is a main concern for the phage application.
Yuanyue Tang, Jie Li, Yuexuan Wang, Zhaojun Song, Hangning Ying, Linghua Kong, Xin’an Jiao, Jinlin Huang   +7 more
doaj   +1 more source

Isolation of Phages and Phage Typing of Bacillus cereus.

Japanese Journal of Food Microbiology, 1998
Bacillus cereusのファージ型別セットを作製した.36株のパイロット株12, 14 を指示菌として, 土壌85検体から96のファージを分離した.保存安定性とパイロット株に対する溶菌パターンから選んだ11のファージは抗血清中和反応により5群に分けられた.そこで, 各群から5ファージ (φa, φb, φc, φd, φeと命名) を選び, 型別セットとした.このセットを用いて, ファージ濃度1×RTD (routine test dilution) および100×RTDでは, 36種のH抗原パイロット株は17の型 (83.3%型別) および11の型 (100%型別) に, 魚粕由来25株は9の型 (48.0%型別) および9の型 (100%型別) に, 土壌由来株100株は24の型 (79.
SHIMIZU, Mikio, INOUE, Matsuhisa, MIYAZAWA, Shichirou, ITOH, Takeshi   +3 more
openaire   +2 more sources

The Type A Phages of Salmonella typhimurium: Observations on Temperate Phage and Lysogenesis [PDF]

Journal of General Microbiology, 1961
SUMMARY: When a standard suspension of Salmonella typhimurium Q1, an indicator strain sensitive to the type A phages recovered from lysogenic strains of this organism. was exposed to concentrations of these phages giving a phage:bacteria ratio of approximately 1:10 (thus ensuring that, with rare exceptions, bacteria became infected with single phage ...
J S, BOYD, D E, BIDWELL
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Northern Normal: Laboratory Networks, Microbial Culture Collections, and Taxonomies of Power (1939–2000)

Engaging Science, Technology, and Society
Bacteriophage-typing – using bacterial viruses to identify bacteria at the species and strain level – was the gold standard technology underlying the rapid expansion of international surveillance for major bacterial pathogens after 1945.
Claas Kirchhelle, Charlotte Kirchhelle
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Transposon Insertion Sequencing Elucidates Novel Gene Involvement in Susceptibility and Resistance to Phages T4 and T7 in Escherichia coli O157

mBio, 2018
Experiments using bacteriophage (phage) to infect bacterial strains have helped define some basic genetic concepts in microbiology, but our understanding of the complexity of bacterium-phage interactions is still limited.
Lauren A. Cowley, Alison S. Low, Derek Pickard, Christine J. Boinett, Timothy J. Dallman, Martin Day, Neil Perry, David L. Gally, Julian Parkhill, Claire Jenkins, Amy K. Cain   +10 more
doaj   +1 more source

Phage typing sets

Mathematical and Computer Modelling, 1992
Epidemiological testing requires fast, accurate and economical techniques. Phage typing is one such technique. Most of the raw data needed for phage testing must be gathered by lots of manual labor without computer assistance. The data that is collected has a random nature and is typically voluminous enough that it is difficult for humans to spot key ...
Markowsky, George, Gershman, Melvin, Hunter, Jacqueline   +2 more
openaire   +1 more source

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source