Results 181 to 190 of about 62,132 (278)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

open access: yesFEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

open access: yesFEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan   +6 more
wiley   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

open access: yesFEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp   +13 more
wiley   +1 more source

Anthropomorphic tissue-mimicking phantoms for oximetry validation in multispectral optical imaging. [PDF]

open access: yesJ Biomed Opt
Dreher KK   +13 more
europepmc   +1 more source

Cystic Ovarian Teratoma: Clinical and Radiographic Insights Into Chronic Pelvic Pain

open access: yesAging and Cancer, EarlyView.
An incidental benign mature cystic ovarian teratoma on imaging and discuss common imaging modalities. ABSTRACT In this case report, we present the clinical course of an 88‐year‐old woman with chronic pelvic pain attributed to an incidental benign mature cystic ovarian teratoma on imaging and discuss common imaging modalities and discussions with ...
Hashim U. Ali   +5 more
wiley   +1 more source

Measurement accuracy of CT systems: The importance of calibration phantoms. [PDF]

open access: yesPLoS One
Scherberich J   +3 more
europepmc   +1 more source

The Associations Between Chronic Active Lesions and White Matter Disease: A 7 Tesla Imaging Study

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background The relationship between paramagnetic rim lesions (PRLs) and surrounding normally appearing white matter (NAWM) disease, potentially contributory to the associations seen between PRLs and clinical impairment, is underexplored. Objectives To assess whether PRLs correlate with a greater degree of NAWM injury in early MS.
Ellie McCluey   +17 more
wiley   +1 more source

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