Results 11 to 20 of about 86,461 (284)

Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli: A Tryptophan and 5-Fluorotryptophan Spectroscopy Study [PDF]

, 2008
In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EIImtl, are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy.
Broos J., Broos J., Broos J., Broos J., Broos J., Callis P. R., Chen Y., Cioni P., Eftink M. R., Galley W. C., Gonnelli M., Gonnelli M., Koning R. I., Lakowicz J. R., Legler P. M., Lengeler J. W., Liu T. Q., Meech S. R., Nguyen T. X., Robillard G. T., Robillard G. T., Ross J. B. A., Strambini G. B., Sugiyama J. E., Swaving Dijkstra D., van Montfort B. A., van Montfort R. L. M., Veldhuis G., Veldhuis G., Veldhuis G., Vervoort E. B., Vos E. P., Winkler G. R.   +32 more
core   +15 more sources

The NADPH oxidase NOX4 regulates redox and metabolic homeostasis preventing HCC progression

Hepatology, EarlyView., 2022
Loss of NOX4 in HCC tumor cells induces metabolic reprogramming in a Nrf2/MYC‐dependent manner to promote HCC progression. Abstract Background and Aims The NADPH oxidase NOX4 plays a tumor‐suppressor function in HCC. Silencing NOX4 confers higher proliferative and migratory capacity to HCC cells and increases their in vivo tumorigenic potential in ...
Irene Peñuelas‐Haro, Rut Espinosa‐Sotelo, Eva Crosas‐Molist, Macarena Herranz‐Itúrbide, Daniel Caballero‐Díaz, Ania Alay, Xavier Solé, Emilio Ramos, Teresa Serrano, María L. Martínez‐Chantar, Ulla G. Knaus, José M. Cuezva, Antonio Zorzano, Esther Bertran, Isabel Fabregat   +14 more
wiley   +1 more source

The Xylulose 5-Phosphate/Phosphate Translocator Supports Triose Phosphate, but Not Phosphoenolpyruvate Transport Across the Inner Envelope Membrane of Plastids in Arabidopsis thaliana Mutant Plants

Frontiers in Plant Science, 2018
The xylulose 5-phosphate/phosphate translocator (PTs) (XPT) represents a link between the plastidial and extraplastidial branches of the oxidative pentose phosphate pathway. Its role is to retrieve pentose phosphates from the extraplastidial space and to
Elke J. A. Hilgers, Pia Staehr, Pia Staehr, Ulf-Ingo Flügge, Rainer E. Häusler   +4 more
doaj   +1 more source

Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt

Microbial Cell Factories, 2021
Background Cyanobacteria are promising hosts for the production of various industrially important compounds such as succinate. This study focuses on introduction of the glyoxylate shunt, which is naturally present in only a few cyanobacteria, into ...
Claudia Durall, Kateryna Kukil, Jeffrey A. Hawkes, Alessia Albergati, Peter Lindblad, Pia Lindberg   +5 more
doaj   +1 more source

Ixodes scapularis Tick Cells Control Anaplasma phagocytophilum Infection by Increasing the Synthesis of Phosphoenolpyruvate from Tyrosine

Frontiers in Cellular and Infection Microbiology, 2017
The obligate intracellular pathogen, Anaplasma phagocytophilum, is the causative agent of life-threatening diseases in humans and animals. A. phagocytophilum is an emerging tick-borne pathogen in the United States, Europe, Africa and Asia, with ...
Alejandro Cabezas-Cruz, Alejandro Cabezas-Cruz, Alejandro Cabezas-Cruz, Pedro J. Espinosa, Dasiel A. Obregón, Pilar Alberdi, José de la Fuente, José de la Fuente   +7 more
doaj   +1 more source

An in vitro Coupled Assay for PEPC with Control of Bicarbonate Concentration

Bio-Protocol, 2021
Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate.
Nicholas Moody, Chatawal Phansopal, James Reid   +2 more
doaj   +1 more source

The promoter polymorphism -232C/G of the PCK1 gene is associated with type 2 diabetes in a UK-resident South Asian population [PDF]

, 2009
Background: The PCK1 gene, encoding cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), has previously been implicated as a candidate gene for type 2 diabetes (T2D) susceptibility.
A Valera, Abigail C Britten, AG Gomez-Valades, AH Barnett, Anthony H Barnett, BF Voight, CJ Willer, EG Beale, EH Hani, H Cao, H Zouali, HD Shin, I Gouni-Berthold, J Paul O'Hare, JC Barrett, L Wegner, M Ann Kelly, S Bellary, S Purcell, SD Rees, Simon D Rees, Srikanth Bellary, Sudhesh Kumar, The International HapMap Consortium, Y Dong   +24 more
core   +6 more sources

Phosphoenolpyruvate Carboxykinase Is Involved in the Decarboxylation of Aspartate in the Bundle Sheath of Maize [PDF]

, 1999
We recently showed that maize (Zea mays L.) leaves contain appreciable amounts of phosphoenolpyruvate carboxykinase (PEPCK; R.P. Walker, R.M. Acheson, L.I. Técsi, R.C. Leegood [1997] Aust J Plant Physiol 24: 459–468).
Chen, Z., Leegood, R.C., Walker, R.P., Wingler, A.   +3 more
core   +2 more sources

Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli

Journal of Hebei University of Science and Technology, 2019
In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE, Zhu2 LI, Liqiang2 LIANG, Wanli MENG, Lantian MIAO, Yaozu SUN, Hongguang2 CAI   +6 more
doaj   +1 more source

Expanding the clinical spectrum of cytosolic phosphoenolpyruvate carboxykinase deficiency: novel PCK1 variants in four Arabian Gulf families

Orphanet Journal of Rare Diseases, 2023
Background In metabolic stress, the cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) enzyme is involved in energy production through the gluconeogenesis pathway.
Marwa Al Busaidi, Feda E. Mohamed, Eiman Al-Ajmi, Nadia Al Hashmi, Khalid Al-Thihli, Amna Al Futaisi, Watfa Al Mamari, Fathiya Al-Murshedi, Fatma Al-Jasmi   +8 more
doaj   +1 more source