Results 21 to 30 of about 45,633 (272)

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Biotechnology for Biofuels, 2020
Background Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules.
Claudia Durall, Pia Lindberg, Jianping Yu, Peter Lindblad   +3 more
doaj   +1 more source

Dibenzylxanthines as PPEPCK-M Inhibitors for Cancer Therapy

Proceedings, 2019
Phosphoenolpyruvate carboxykinase (PEPCK) is the key enzyme in gluconeogenesis/glyceroneogenesis, which catalyzes the decarboxylation of oxaloacetate to phosphoenolpyruvate. [...]
Marc Aragó, Sergio Rodríguez-Arévalo, Petra Hyrossova, Juan Moreno, Sònia Abás, Agnès Figueras, Belén Pérez, Francesc Viñals, María Carmen Escolano, José Carlos Perales   +9 more
doaj   +1 more source

Phosphoenolpyruvate regulates the Th17 transcriptional program and inhibits autoimmunity

Cell Reports, 2023
Summary: Aerobic glycolysis, a metabolic pathway essential for effector T cell survival and proliferation, regulates differentiation of autoimmune T helper (Th) 17 cells, but the mechanism underlying this regulation is largely unknown.
Tsung-Yen Huang, Masato Hirota, Daiki Sasaki, Rajkumar Singh Kalra, Hsiao-Chiao Chien, Miho Tamai, Shukla Sarkar, Yang Mi, Mio Miyagi, Yu Seto, Hiroki Ishikawa   +10 more
doaj   +1 more source

Expanding the clinical spectrum of cytosolic phosphoenolpyruvate carboxykinase deficiency: novel PCK1 variants in four Arabian Gulf families

Orphanet Journal of Rare Diseases, 2023
Background In metabolic stress, the cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C) enzyme is involved in energy production through the gluconeogenesis pathway.
Marwa Al Busaidi, Feda E. Mohamed, Eiman Al-Ajmi, Nadia Al Hashmi, Khalid Al-Thihli, Amna Al Futaisi, Watfa Al Mamari, Fathiya Al-Murshedi, Fatma Al-Jasmi   +8 more
doaj   +1 more source

Phosphoenolpyruvate carboxykinases as emerging targets in cancer therapy

Frontiers in Cell and Developmental Biology, 2023
Metabolic reprogramming is commonly accompanied by alterations in the expression of metabolic enzymes. These metabolic enzymes not only catalyze the intracellular metabolic reaction, but also participate in a series of molecular events to regulate tumor ...
Yong Yu, Jingying Li, Kaiming Ren
doaj   +1 more source

Acetate Metabolism in Archaea: Characterization of an Acetate Transporter and of Enzymes Involved in Acetate Activation and Gluconeogenesis in Haloferax volcanii

Frontiers in Microbiology, 2020
The haloarchaeon Haloferax volcanii grows on acetate as sole carbon and energy source. The genes and proteins involved in uptake and activation of acetate and in gluconeogenesis were identified and analyzed by characterization of enzymes and by growth ...
Tom Kuprat, Ulrike Johnsen, Marius Ortjohann, Peter Schönheit   +3 more
doaj   +1 more source

Extraction and Measurement the Activities of Cytosolic Phosphoenolpyruvate Carboxykinase (PEPCK) and Plastidic NADP-dependent Malic Enzyme (ME) on Tomato (Solanum lycopersicum)

Bio-Protocol, 2014
A recent study demonstrated that cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and NADP-malic enzyme (NADP-ME) have an important role in malate metabolism during fruit ripening (Osorio et al., 2013). PEPCK catalyze the ATP-dependent decarboxylation
Sonia Osorio, José Vallarino, Marek Szecowka, Shai Ufaz, Vered Tzin, Ruthie Angelovici, Gad Galili, Fayezeh Aarabi   +7 more
doaj   +1 more source

Application of a Novel and Automated Branched DNA in Situ Hybridization Method for the Rapid and Sensitive Localization of mRNA Molecules in Plant Tissues

Applications in Plant Sciences, 2014
Premise of the study: A novel branched DNA detection technology, RNAscope in situ hybridization (ISH), originally developed for use on human clinical and animal tissues, was adapted for use in plant tissue in an attempt to overcome some of the ...
Andrew J. Bowling, Heather E. Pence, Jeffrey B. Church   +2 more
doaj   +1 more source

Comprehensive Analysis of Malate Accumulation in Peaches in Response to Cold Stress Based on Transcriptomics. [PDF]

Food Sci Nutr
This study explored malate‐accumulation mechanisms in “Hujingmilu” peaches under different storage conditions. Low temperature (LT) delayed firmness decline and maintained higher malate. Transcriptomics showed malate biosynthetic genes weakly correlate with malate levels, while proton pump and transporter genes were up‐regulated under LT.
Cai H, Han S, Wang X, Shi J, Xiong Z, Zhang Z, Gao Z.   +6 more
europepmc   +2 more sources

Intramitochondrial Metabolism of Phosphoenolpyruvate

Journal of Biological Chemistry, 1967
Abstract Phosphoenolpyruvate was formed by rabbit liver mitochondria during incubations in the presence of dinitrophenol with α-ketoglutarate and with other Krebs cycle intermediates as substrates. These intermediates, as indicated by experiments with labeled compounds, served as precursors for the carbon skeleton of the synthesized phosphoenolpyruvate.
J L, Gamble, J A, Mazur
openaire   +2 more sources