Results 71 to 80 of about 39,501 (165)
Purification, partial kinetic characterization and reactive sulfhydryl groups of the phosphoenolpyruvate carboxykinase from Perumytilus purpuratus adductor muscle [PDF]
, 1995 Phosphoenolpyruvate carboxykinase (PEPCK) from the adductor muscle of Perumytilus purpuratus was purified to homogeneity, as determined by SDS-polyacrylamide gel electrophoresis (PAGE).
Robert W. Simpfendörfer +7 more
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Involvement of HNF-1 in the regulation of phosphoenolpyruvate carboxykinase gene expression in the kidney [PDF]
, 1997 The cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene is differentially expressed in several tissues. A specific set of regulatory elements in the promoter are responsible for the control of PEPCK gene transcription and, in turn ...
Hanoch Cassuto +11 more
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Journal of Biological Chemistry, 1982 The mRNA that codes for phosphoenolpyruvate carboxykinase accounts for approximately 0.2% of the protein synthesized in H4IIEC3 hepatoma cells maintained for 24 h in serum-free medium containing N6,O2'-dibutyryl cAMP and theophylline. This value decreases to 0.04% within 3 h after the addition of insulin.
T L, Andreone +3 more
openaire +2 more sources
Fluorescent labeling of the nucleotide site in cytosolic rat liver phosphoenolpyruvate carboxykinase [PDF]
, 1991 Reaction of rat liver phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32) with the alkylating fluorescent probe N-(iodoacetylaminoethyl)-5-naphthylamine-1-sulfonic acid (1,5-I-AEDANS), results in ...
Encinas, M. Victoria +2 more
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Journal of Biological Chemistry, 1989 Transcription of the gene for the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) (EC 4.1.1.32) (PEPCK) from the rat is acutely regulated by a number of hormones, including glucagon (acting via cAMP), glucocorticoids, and insulin. In this study we demonstrate by DNase I footprinting that a region of the PEPCK promoter, extending from -460 to +
W J, Roesler +2 more
openaire +2 more sources
, 1987 A phosphoprotein of 65 kDa, as determined by SDS-gel electrophoresis, has been isolated from yeast crude extracts. This phospho form copurifies with phosphoenolpyruvate carboxykinase in the enzyme purification procedure worked out in our laboratory ...
M. Radrizzani +4 more
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, 1992 A procedure was established for the rapid isolation of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase (PEPCK) from an overproducing strain.
Rose, M. +11 more
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, 1988 A highly specific and sensitive assay for the determination of phosphoenolpyruvate carboxykinase (PEPCK) in nanogram-sized tissue samples is described. This test system is based on the stoichiometric transformation of phosphoenolpyruvate into ATP.
Wimmer M
core +1 more source
, 1982 Incubation of partially purified yeast phosphoenolpyruvate carboxykinase (ATP: oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49) with 5% mercaptoethanol and 0.01% sodium dodecyl sulfate at 37°C results in degradation of the enzyme.
Müller, M.P.A., Holzer, H., Beck, I.
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Production of Succinic Acid Using Phosphoenolpyruvate Carboxykinase in Escherichia coli
, 2010 Succinic acid has been of interest with its various uses as a precursor for industrially useful chemicals, pharmaceuticals, and biopolymers. Native succinic acid producers including Mannheimia succiniciproducens were found to produce phosphoenolpyruvate (
Lee, Kyung Yun +4 more
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