Cloning and expression of phosphoenolpyruvate carboxykinase from Escherichia coli
Journal of Hebei University of Science and Technology, 2019 In order to better study the structure and function of phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase gene is cloned and expressed by PCR (polymerase chain reaction), double enzyme digestion and cell transformation. The results show
Xiaoliang HE +6 more
doaj +1 more source
Purification and characterization of phosphoenolpyruvate carboxylase from a cyanobacterium [PDF]
, 1986 Phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31) was purified 100-fold from the cyanobacterium Coccochloris peniocystis with a yield of 10%. A single isozyme was found at all stages of purification, and activity of other beta-carboxylase enzymes was ...
Colman, Brian., Owttrim, George W.
core +1 more source
Effect of Nitrate and Ammonium Nutrition of Nonnodulated Phaseolus vulgaris L. on Phosphoenolpyruvate Carboxylase and Pyruvate Kinase Activity [PDF]
, 1985 Young bean plants (Phaseolus vulgaris L. var Saxa) were fed with 3.5 or 10 millimolar N in either the form of NO3− or NH4+, after being grown on N-free nutrient solution for 8 days. The pH of the nutrient solutions was either 6 or 4.
Erismann, Karl Hans, Schweizer, Patrick
core +1 more source
Molecular Plant-Microbe Interactions, 2003 Phosphoenolpyruvate carboxylases (PEPCs), one form of which in each legume species plays a central role in the carbon metabolism in symbiotic root nodules, are activated through phosphorylation of a conserved residue by a specific protein kinase (PEPC-PK)
Tomomi Nakagawa +6 more
doaj +1 more source
Glucocorticoid exposure in late gestation permanently programs rat hepatic phosphoenolpyruvate carboxykinase and glucocorticoid receptor expression and causes glucose intolerance in adult offspring [PDF]
, 1998 Low birth weight in humans is predictive of insulin resistance and diabetes in adult life. The molecular mechanisms underlying this link are unknown but fetal exposure to excess glucocorticoids has been implicated, The fetus is normally protected from ...
Kenyon, C J +9 more
core +1 more source
Metabolic Engineering Communications, 2015 The effect of pyruvate kinase gene (pyk) deletion on the physiology of Corynebacterium glutamicum ATCC13032 was investigated under biotin-sufficient, non-glutamate-producing conditions.
Kazunori Sawada +5 more
doaj +1 more source
Effect of salicylic acid pretreatment on cadmium toxicity in wheat
Biologia Plantarum, 2010 Cadmium (100, 400 and 1000 μM CdCl2) treatments resulted in the inhibition of root dry biomass, root elongation and increased Cd accumulation in wheat (Triticum aestivum L.) roots.
H. R. Moussa, S. M. El-Gamal
doaj +1 more source
Evidence of coexistence of C₃ and C₄ photosynthetic pathways in a green-tide-forming alga, Ulva prolifera. [PDF]
PLoS ONE, 2012 Ulva prolifera, a typical green-tide-forming alga, can accumulate a large biomass in a relatively short time period, suggesting that photosynthesis in this organism, particularly its carbon fixation pathway, must be very efficient.
Jianfang Xu +8 more
doaj +1 more source
Abscisic acid as a factor in regulation of photosynthetic carbon metabolism of pea seedlings
Acta Societatis Botanicorum Poloniae, 2014 The influence of abscisic acid (ABA) on carbon metabolism and the activity of ribulosebisphosphate (RuBP) and phosphoenolpyruvate (PEP) carboxylases in 8-day-old pea seedlings was investigated.
Maria Faltynowicz +2 more
doaj +1 more source
Journal of Plant Interactions, 2022 Karstification forms tremendous karst carbon sinks in the Earth. Whether terrestrial higher plants can absorb and utilize bicarbonate, there is a key testimony that karst carbon sinks can be transformed into carbon sequestrations by terrestrial higher ...
Lei Fang, Yanyou Wu
doaj +1 more source