Results 131 to 140 of about 2,068 (170)
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Phosphoenolpyruvate carboxylase regulation in C4‐PEPC‐expressing transgenic rice during early responses to drought stress

Physiologia Plantarum, 2016
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) has important functions in C4 photosynthesis and biosynthesis of intermediate metabolites. In this study, the drought resistance of C4‐PEPC‐expressing transgenic rice (Oryza sativa, line PC) plants was assessed using simulated drought conditions [i.e. polyethylene glycol (PEG)‐6000 treatment]. The dry
Xiaolong, Liu   +6 more
openaire   +2 more sources

Studying allelic diversity and stress response of PEPC (Phosphoenolpyruvate carboxylase) in Norway spruce (Picea abies)

Chemosphere, 1998
Abstract A novel experimental approach in ecological genetics for analysing allelic diversity at an isoenzyme-gene-system is presented. This approach is based on the question how individual genetic diversity is implemented in adaptive processes towards stress.
A. Ipsen   +3 more
openaire   +1 more source

Extrinsic thermostabilization factors and thermodenaturation mechanisms for phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis

Journal of Fermentation and Bioengineering, 1997
The thermodenaturation of phosphoenolpyruvate carboxylase (PEPC) from an extremely thermophilic bacterium Rhodothermus obamensis, capable of growth at temperature up to 85°C, was probed at different denaturation temperatures by UV-visible absorption, fluorescence emission and 1-anilinonaphthalene-8-sulfonate (ANS) binding and renaturation was assessed ...
Ken Takai   +2 more
openaire   +1 more source

Physiological characteristics and metabolomics of transgenic wheat containing the maize C4 phosphoenolpyruvate carboxylase (PEPC) gene under high temperature stress

Protoplasma, 2016
In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome.
Xueli, Qi   +8 more
openaire   +2 more sources

Expression of Maize Gene Encoding C4-Pyruvate Orthophosphate Dikinase (PPDK) and C4-Phosphoenolpyruvate Carboxylase (PEPC) in Transgenic Arabidopsis

Plant Molecular Biology Reporter, 2012
To improve the efficiency of CO2 fixation in C3 photosynthesis, C4-cycle genes were overexpressed in Arabidopsis plants. In this study, a full-length cDNA for pyruvate orthophosphate dikinase (PPDK) with 971 amino acids was isolated from Zea mays using LA-PCR.
Yu-Min Wang   +5 more
openaire   +1 more source

Pyramiding expression of maize genes encoding phosphoenolpyruvate carboxylase (PEPC) and pyruvate orthophosphate dikinase (PPDK) synergistically improve the photosynthetic characteristics of transgenic wheat

Protoplasma, 2014
Using particle bombardment transformation, we introduced maize pepc cDNA encoding phosphoenolpyruvate carboxylase (PEPC) and ppdk cDNA encoding pyruvate orthophosphate dikinase (PPDK) into the C3 crop wheat to generate transgenic wheat lines carrying cDNA of pepc (PC lines), ppdk (PK lines) or both (PKC lines).
HuiFang, Zhang   +8 more
openaire   +2 more sources

Tree stem phosphoenolpyruvate carboxylase (PEPC): lack of biochemical and localization evidence for a C4‐like photosynthesis system

New Phytologist, 2007
Here, the kinetic properties and immunolocalization of phosphoenolpyruvate carboxylase (PEPC) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in young stems of Fagus sylvatica were investigated. The aim of the study was to test the hypothesis that there is a C4-like photosynthesis system in the stems of this C3 tree species. The activity,
Daniel, Berveiller   +6 more
openaire   +2 more sources

Recombinant thermoactive phosphoenolpyruvate carboxylase (PEPC) from Thermosynechococcus elongatus and its coupling with mesophilic/thermophilic bacterial carbonic anhydrases (CAs) for the conversion of CO2 to oxaloacetate

Bioorganic & Medicinal Chemistry, 2016
With the continuous increase of atmospheric CO2 in the last decades, efficient methods for carbon capture, sequestration, and utilization are urgently required. The possibility of converting CO2 into useful chemicals could be a good strategy to both decreasing the CO2 concentration and for achieving an efficient exploitation of this cheap carbon source.
DEL PRETE, SONIA   +4 more
openaire   +4 more sources

Discovery of Novel Phosphoenolpyruvate Carboxylase (PEPC) Genes and Their Active Polypeptides in the Green Microalga Chlamydomonas reinhardtii

2010
This work describes the discovery of novel phosphoenolpyruvate carboxylase (PEPC) genes and theiractive catalitic polypeptides in the green microalga Chlamydomonas reinhardhii. Green-algal PEPC hasbeen unexplored before in molecular terms. Our recent studies have reported the molecular cloning ofthe two PEPC genes, Ppc genes in Creinhardtii (CrPpcl ...
Tarlan G. Mamedov, Raymond Chollet
openaire   +1 more source

Exogenous ATP enhance signal response of suspension cells of transgenic rice (Oryza sativa L.) expressing maize C 4 -pepc encoded phosphoenolpyruvate carboxylase under PEG treatment

Plant Growth Regulation, 2016
The aim of this study was to analyze the biochemical and molecular responses of suspension cells of transgenic rice (Oryza sativa L.) over-expressing the maize C 4 -pepc gene encodes phosphoenolpyruvate carboxylase (PEPC) (PC line) to drought stress by exogenous ATP ...
K. Huo   +6 more
openaire   +1 more source

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