CBIO-15NON-METABOLIC FUNCTION OF PHOSPHOFRUCTOKINASE-1 IN GLIOBLASTOMA MAINTENANCE [PDF]
Neuro Oncol, 2015 BACKGROUND: Glioblastoma multiforme (GBM) remains one of the most lethal tumors and is associated with a median survival of only approximately 15 months, despite aggressive combination radio-chemo-therapy. Regardless of recent advances in our understanding of this deadly disease, the molecular mechanisms/genes that cause high recurrence rates and ...
Jensen K +8 more
europepmc +4 more sources
Analysis of phosphofructokinase-1 activity as affected by pH and ATP concentration [PDF]
Scientific ReportsA key player in energy metabolism is phosphofructokinase-1 (PFK1) whose activity and behavior strongly influence glycolysis and thus have implications in many areas. In this research, PFK1 assays were performed to convert F6P and ATP into F-1,6-P and ADP
Chengcheng Wang +6 more
doaj +4 more sources
Phosphofructokinase-1 redefined: a metabolic hub orchestrating cancer hallmarks through multi-dimensional control networks [PDF]
Journal of Translational MedicinePhosphofructokinase-1 (PFK-1), the core rate-limiting enzyme of glycolysis, has transcended its classical metabolic regulatory role and emerged as a multi-dimensional hub in tumour biology.
Rong Yuan +4 more
doaj +4 more sources
Phosphofructokinase 1 Glycosylation Regulates Cell Growth and Metabolism [PDF]
Science, 2012 Cancer cells must satisfy the metabolic demands of rapid cell growth within a continually changing microenvironment. We demonstrated that the dynamic posttranslational modification of proteins by O-linked β-N-acetylglucosamine (O-GlcNAcylation) is a key ...
Clark, Peter M. +8 more
core +6 more sources
How Phosphofructokinase-1 Promotes PI3K and YAP/TAZ in Cancer: Therapeutic Perspectives. [PDF]
Cancers (Basel), 2022 PI3K/AKT is one of the most frequently altered signaling pathways in human cancers, supporting the activation of many proteins sustaining cell metabolism, proliferation, and aggressiveness. Another important pathway frequently altered in cancer cells is the one regulating the YAP/TAZ transcriptional coactivators, which promote the expression of genes ...
Simula L, Alifano M, Icard P.
europepmc +3 more sources
Structures of Human Phosphofructokinase-1 and Atomic Basis of Cancer-Associated Mutations [PDF]
Biophysical Journal, 2016 Phosphofructokinase-1 (PFK1), the 'gatekeeper' of glycolysis, catalyses the committed step of the glycolytic pathway by converting fructose-6-phosphate to fructose-1,6-bisphosphate. Allosteric activation and inhibition of PFK1 by over ten metabolites and in response to hormonal signalling fine-tune glycolytic flux to meet energy requirements. Mutations
Bradley A. Webb +5 more
openalex +6 more sources
Capillary electrophoresis-based assay of phosphofructokinase-1. [PDF]
Anal Biochem, 2014 An assay was developed for phosphofructokinase-1 (PFK-1) using capillary electrophoresis (CE). In the glycolytic pathway, this enzyme catalyzes the rate-limiting step from fructose-6-phosphate and magnesium-bound adenosine triphosphate (Mg-ATP) to fructose-1,6-bisphosphate and magnesium-bound adenosine diphosphate (Mg-ADP).
Malina A +4 more
europepmc +4 more sources
Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways [PDF]
Microbial Cell Factories, 2019 Background The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity ...
José M. Pastor +12 more
doaj +3 more sources
Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. [PDF]
Neurosci Bull, 2016 Phosphofructokinase-1 (PFK-1), a major regulatory glycolytic enzyme, has been implicated in the functions of astrocytes and neurons. Here, we report that PFK-1 negatively regulates neurogenesis from neural stem cells (NSCs) by targeting pro-neural transcriptional factors.
Zhang F +7 more
europepmc +4 more sources
The Glycolytic Enzyme Phosphofructokinase-1 Assembles into Filaments [PDF]
Biophysical Journal, 2018 Despite abundant knowledge of the regulation and biochemistry of glycolytic enzymes, we have limited understanding on how they are spatially organized in the cell. Emerging evidence indicates that nonglycolytic metabolic enzymes regulating diverse pathways can assemble into polymers.
Bradley A. Webb +4 more
openalex +6 more sources