Capillary electrophoresis-based assay of phosphofructokinase-1. [PDF]
Anal Biochem, 2014 An assay was developed for phosphofructokinase-1 (PFK-1) using capillary electrophoresis (CE). In the glycolytic pathway, this enzyme catalyzes the rate-limiting step from fructose-6-phosphate and magnesium-bound adenosine triphosphate (Mg-ATP) to fructose-1,6-bisphosphate and magnesium-bound adenosine diphosphate (Mg-ADP).
Malina A +4 more
europepmc +7 more sources
The glycolytic enzyme phosphofructokinase-1 assembles into filaments. [PDF]
J Cell Biol, 2017 Despite abundant knowledge of the regulation and biochemistry of glycolytic enzymes, we have limited understanding on how they are spatially organized in the cell.
Webb BA +4 more
europepmc +10 more sources
CBMT-47. THE ROLE OF PHOSPHOFRUCTOKINASE-1 IN GLIOBLASTOMA MAINTENANCE AND MOTILITY [PDF]
Neuro Oncol, 2018 Jensen K +9 more
europepmc +5 more sources
Protein phosphatase 4 dephosphorylates phosphofructokinase-1 to regulate its enzymatic activity. [PDF]
BMB Rep, 2023 Most cancer cells utilize glucose at a high rate to produce energy and precursors for the biosynthesis of macromolecules such as lipids, proteins, and nucleic acids. This phenomenon is called the Warburg effect or aerobic glycolysis— this distinct characteristic is an attractive target for developing anticancer drugs.
Park J, Lee DH.
europepmc +4 more sources
Phosphofructokinase-1 and fructose bisphosphatase-1 in canine liver and kidney. [PDF]
J Vet Med Sci, 2019 In healthy individuals, plasma glucose levels are maintained within a normal range. During fasting, endogenous glucose is released either through glycogenolysis or gluconeogenesis. Gluconeogenesis involves the formation of glucose-6-phosphate from a variety of precursors followed by its subsequent hydrolysis to glucose.
Kanai S +3 more
europepmc +6 more sources
Phosphofructokinase-1 Negatively Regulates Neurogenesis from Neural Stem Cells. [PDF]
Neurosci Bull, 2016 Phosphofructokinase-1 (PFK-1), a major regulatory glycolytic enzyme, has been implicated in the functions of astrocytes and neurons. Here, we report that PFK-1 negatively regulates neurogenesis from neural stem cells (NSCs) by targeting pro-neural transcriptional factors.
Zhang F +7 more
europepmc +7 more sources
Phosphofructokinase 1 Glycosylation Regulates Cell Growth and Metabolism [PDF]
Science, 2012 Cancer cells must satisfy the metabolic demands of rapid cell growth within a continually changing microenvironment. We demonstrated that the dynamic posttranslational modification of proteins by O-linked β-N-acetylglucosamine (O-GlcNAcylation) is a key ...
Clark, Peter M. +8 more
core +8 more sources
CBIO-15NON-METABOLIC FUNCTION OF PHOSPHOFRUCTOKINASE-1 IN GLIOBLASTOMA MAINTENANCE [PDF]
Neuro Oncol, 2015 BACKGROUND: Glioblastoma multiforme (GBM) remains one of the most lethal tumors and is associated with a median survival of only approximately 15 months, despite aggressive combination radio-chemo-therapy. Regardless of recent advances in our understanding of this deadly disease, the molecular mechanisms/genes that cause high recurrence rates and ...
Jensen K +8 more
europepmc +5 more sources
Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations. [PDF]
Nature, 2015 Webb BA +5 more
europepmc +5 more sources
Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways [PDF]
Microbial Cell Factories, 2019 Background The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner–Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity ...
José M. Pastor +12 more
doaj +3 more sources