Results 201 to 210 of about 21,592 (215)

Properties of phosphofructokinase 1 fromPythium ultimum andAgaricus bisporus and comparison with other fungal phosphofructokinases 1

Experimental Mycology, 1991
Abstract Phosphofructokinase 1 was partially purified from Agaricus bisporus fruit bodies and Pythium ultimum mycelium. The enzyme from A. bisporus was similar to other fungal phosphofructokinases 1: it was inhibited by ATP, had sigmoidal kinetics toward fructose-6-phosphate, and was stimulated by fructose-2,6-bisphosphate. Phosphoenolpyruvate was
Andre´J. Van Laere, Hilde M. Joosen
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Phosphofructokinase 1 platelet isoform induces PD-L1 expression to promote glioblastoma immune evasion

Genes & Genomics, 2022
Overexpression of PD-L1 is observed in many types of human cancer, including glioblastoma (GBM) and contributes to tumor immune evasion. In addition, GBM shows highly-activated aerobic glycolysis due to overexpression of phosphofructokinase 1 platelet isoform (PFKP), which the key enzyme in the glycolysis.
Silu Wang, Su Hwan Park, Je Sun Lim, Yun-Yong Park, Linyong Du, Jong-Ho Lee   +5 more
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Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus

Biochemical and Biophysical Research Communications, 1991
Phosphofructokinase 1 (PFK1) from Thermus thermophilus differs from other bacterial PFKs in that it is regulated by effector-induced reversible tetramer-dimer conversion rather than conformational change alone. We have cloned its gene and the deduced amino acid sequence was compared with that of other PFKs. While almost all amino acid residues involved
J, Xu, M, Seki, K, Denda, M, Yoshida
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Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation

Biochemical Journal, 2015
Phosphofructokinase-1 (Pfk) acts as the main control point of flux through glycolysis. It is involved in complex allosteric regulation and Pfk mutations have been linked to cancer development. Whereas the 3D structure and structural basis of allosteric regulation of prokaryotic Pfk has been studied in great detail, our knowledge about the molecular ...
Marco, Kloos, Antje, Brüser, Jürgen, Kirchberger, Torsten, Schöneberg, Norbert, Sträter   +4 more
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A radioassay for phosphofructokinase-1 activity in cell extracts and purified enzyme

Journal of Biochemical and Biophysical Methods, 2002
Phosphofructokinase-1 plays a key role in the regulation of carbohydrate metabolism. Its activity can be used as an indicator of the glycolytic flux in a tissue sample. The method most commonly employed to determine phosphofructokinase-1 activity is based on oxidation of NADH by the use of aldolase, triosephosphate isomerase, and alpha-glycerophosphate
Mauro, Sola-Penna, Ana Cristina, dos Santos, Gutemberg G, Alves, Tatiana, El-Bacha, Joana, Faber-Barata, Monica F, Pereira, Fredson C, Serejo, Andrea T, Da Poian, Martha, Sorenson   +8 more
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Assembly of phosphofructokinase-1 fromSaccharomyces cerevisiae in extracts of single-deletion mutants

Yeast, 1998
Phosphofructokinase-1 from Saccharomyces cerevisiae is an octameric enzyme comprising two non-identical subunits, alpha and beta, which are encoded by the unlinked genes PFK1 and PFK2. In this paper, assembly and reactivation of the enzyme have been studied in cell-free extracts of single-deletion mutants.
A, Klinder, J, Kirchberger, A, Edelmann, G, Kopperschläger   +3 more
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Phosphofructokinase-1 in Cancer: A Promising Target for Diagnosis and Therapy

Recent Patents on Anti-Cancer Drug Discovery
Tumor cells have distorted enzymatic houses, which change the metabolic state from oxidative phosphorylation to glycolysis with high lactate levels in a hypoxic environment. Redrafting the metabolic profile is an emerging hallmark of cancer.
Ali Raza Ishaq, Tahira Younis, Shankun Lin, Muhammad Usman, Tingfang Wang, Zhe-Sheng Chen   +5 more
openaire   +2 more sources

Novel testis- and embryo-specific isoforms of the phosphofructokinase-1 muscle type gene

Biochemical and Biophysical Research Communications, 2004
We have identified novel transcriptional isoforms of the human and mouse genes encoding muscle type phosphofructokinase-1 (PFK-M). These isoforms are expressed specifically in the testis and in the mid-gestation embryo, and have been termed TE-PFK-M (testis- and embryo-specific PFK-M).
Satoru, Yamada, Hiromu, Nakajima, Michael R, Kuehn   +2 more
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Basal and insulin-mediated carbohydrate metabolism in human muscle deficient in phosphofructokinase 1

American Journal of Physiology-Endocrinology and Metabolism, 1991
Biopsies were obtained from the quadriceps femoris muscle of two male patients deficient in phosphofructokinase (PFK) 1. In the basal state the patients had markedly higher contents of UDP-glucose (approximately 5-fold), hexose monophosphates (approximately 7- to 13-fold), inosine monophosphate (IMP) (approximately 15-fold), and fructose 2,6 ...
Katz, A, Spencer, M, Lillioja, Stephen, Yan, Z, Mott, D, Haller, R, Lewis, S   +6 more
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Regulation of Phosphofructokinase-1 on Submandibular Salivary Glands of Rats after Isoproterenol Administration

Archives of Physiology and Biochemistry, 2000
The purpose of this investigation was to study the effect of isoproterenol (IPR) treatment on the regulation of phosphofructokinase-1 of submandibular salivary glands of rats. The animals were divided into control and experimental groups. In the first set of experiments, the rats received 5 mg of IPR/kg b.w.
J, Nicolau, D N, Souza, G, Nunez-Burgos
openaire   +2 more sources