Results 201 to 210 of about 34,103 (213)
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A thermostable phosphofructokinase from the extreme thermophile Thermus X-1
Archives of Biochemistry and Biophysics, 1975Abstract Two relatively simple procedures are described for the purification of phosphofructokinase from the extreme thermophile, Thermus X-1. The native enzyme has a molecular weight of 1.32 × 10 5 and contains four apparently identical polypeptide chains.
K H, Cass, E, Stellwagen
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Phosphofructokinase Isozymes in Pancreatic Islets and Clonal β-Cells (INS-1)
Diabetes, 1995Normal insulin secretion is oscillatory in vivo, and the oscillations are impaired in type II diabetes. We and others have shown oscillations in insulin secretion from isolated perifused islets stimulated with glucose, and in this study we show oscillations in insulin secretion from the glucose-sensitive clonal β-cell line INS-1.
G C, Yaney +5 more
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Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus
Biochemical and Biophysical Research Communications, 1991Phosphofructokinase 1 (PFK1) from Thermus thermophilus differs from other bacterial PFKs in that it is regulated by effector-induced reversible tetramer-dimer conversion rather than conformational change alone. We have cloned its gene and the deduced amino acid sequence was compared with that of other PFKs. While almost all amino acid residues involved
J, Xu, M, Seki, K, Denda, M, Yoshida
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Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation
Biochemical Journal, 2015Phosphofructokinase-1 (Pfk) acts as the main control point of flux through glycolysis. It is involved in complex allosteric regulation and Pfk mutations have been linked to cancer development. Whereas the 3D structure and structural basis of allosteric regulation of prokaryotic Pfk has been studied in great detail, our knowledge about the molecular ...
Marco, Kloos +4 more
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A radioassay for phosphofructokinase-1 activity in cell extracts and purified enzyme
Journal of Biochemical and Biophysical Methods, 2002Phosphofructokinase-1 plays a key role in the regulation of carbohydrate metabolism. Its activity can be used as an indicator of the glycolytic flux in a tissue sample. The method most commonly employed to determine phosphofructokinase-1 activity is based on oxidation of NADH by the use of aldolase, triosephosphate isomerase, and alpha-glycerophosphate
Mauro, Sola-Penna +8 more
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Phosphofructokinase type 1 kinetics, isoform expression, and gene polymorphisms in cancer cells
Journal of Cellular Biochemistry, 2012AbstractKinetic analysis of PFK‐1 from rodent AS‐30D, and human HeLa and MCF‐7 carcinomas revealed sigmoidal [fructose 6‐phosphate, Fru6P]‐rate curves with different Vm values when varying the allosteric activator fructose 2,6 bisphosphate (Fru2,6BP), AMP, Pi, NH, or K+. The rate equation that accurately predicted this behavior was the exclusive ligand
Rafael, Moreno-Sánchez +6 more
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Phosphofructokinase-1 in Cancer: A Promising Target for Diagnosis and Therapy
Recent Patents on Anti-Cancer Drug DiscoveryTumor cells have distorted enzymatic houses, which change the metabolic state from oxidative phosphorylation to glycolysis with high lactate levels in a hypoxic environment. Redrafting the metabolic profile is an emerging hallmark of cancer.
Ali Raza Ishaq +5 more
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Yeast, 1998
Phosphofructokinase-1 from Saccharomyces cerevisiae is an octameric enzyme comprising two non-identical subunits, alpha and beta, which are encoded by the unlinked genes PFK1 and PFK2. In this paper, assembly and reactivation of the enzyme have been studied in cell-free extracts of single-deletion mutants.
A, Klinder +3 more
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Phosphofructokinase-1 from Saccharomyces cerevisiae is an octameric enzyme comprising two non-identical subunits, alpha and beta, which are encoded by the unlinked genes PFK1 and PFK2. In this paper, assembly and reactivation of the enzyme have been studied in cell-free extracts of single-deletion mutants.
A, Klinder +3 more
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Fructokinase Assay with a Specific Spectrophotometric Method using 1-Phosphofructokinase
Enzymologia biologica et clinica, 1967M A, Günther, A, Sillero, A, Sols
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