Results 211 to 220 of about 244,532 (236)
Some of the next articles are maybe not open access.
A simplified assay for phospholipase C
Analytical Biochemistry, 1979Abstract A new assay for phospholipase C activity that uses alkaline phosphatase to convert phosphorylcholine to inorganic phosphate is described. The determination of inorganic phosphate is performed in the presence of phosphatidylcholine and protein after the addition of sodium dodecyl sulfate.
Nancy J. Truesdale +2 more
openaire +3 more sources
Aspirin inhibits phospholipase C
Biochemical and Biophysical Research Communications, 1986We have shown previously that aspirin (ASA) ingestion by normal human volunteers inhibits peripheral blood monocyte phospholipase C (PLC) activities ex vivo. In order to explore further the mechanism of action of ASA, normal human monocytes and differentiated human U937 cells were treated with ASA and other salicylates. Cells preincubated with ASA were
Mike A. Clark +2 more
openaire +3 more sources
2012
Phospholipase C (PLC) family members constitute a family of diverse enzymes. Thirteen different family members have been cloned. These family members have unique structures that mediate diverse functions. Although PLC family members all appear to signal through the bi-products of cleaving phospholipids, it is clear that each family member, and at times
openaire +2 more sources
Phospholipase C (PLC) family members constitute a family of diverse enzymes. Thirteen different family members have been cloned. These family members have unique structures that mediate diverse functions. Although PLC family members all appear to signal through the bi-products of cleaving phospholipids, it is clear that each family member, and at times
openaire +2 more sources
The physiological roles of primary phospholipase C
Advances in Biological Regulation, 2013The roles of phosphoinositide-specific phospholipase C (PLC) have been extensively investigated in diverse cell lines and pathological conditions. Among the PLC isozmes, primary PLCs, PLC-β and PLC-γ, are directly activated by receptor activation, unlike other secondary PLCs (PLC-ɛ, PLC-δ1, and PLC-η1).
Yang YR +3 more
openaire +4 more sources
2003
Publisher Summary This chapter provides an overview of mammalian phospholipase C. The hydrolysis of cellular phospholipids leads to the formation of various bioactive lipid mediators, acting either as extracellular signaling molecules or as intracellular second messengers. A well-known second messenger-forming system involving phospholipid hydrolysis
Martina Schmidt +4 more
openaire +2 more sources
Publisher Summary This chapter provides an overview of mammalian phospholipase C. The hydrolysis of cellular phospholipids leads to the formation of various bioactive lipid mediators, acting either as extracellular signaling molecules or as intracellular second messengers. A well-known second messenger-forming system involving phospholipid hydrolysis
Martina Schmidt +4 more
openaire +2 more sources
Purification of Phospholipase C β and Phospholipase C ε from Sf9 Cells
2003Phosphatidylinositol-specific phospholipase C (PLC) enzymes catalyze hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) generating diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP(3)). The PLC beta isoforms of PLCs are activated by G proteins after hormone or neurotransmitter stimulation of G protein-coupled receptors (GPCR).
Huan Wang +3 more
openaire +3 more sources
[22] Bioluminescent assay for lipase, phospholipase A2, and phospholipase C
1981Publisher Summary This chapter describes the bioluminescent assay for lipase, phospholipase A 2 , and phospholipase C. The dim mutant M17 of the marine luminous bacterium Beneckea harveyi MAV #392 emits light with either exogenous aldehydes or fatty acids.
Michael Heller, Shimon Ulitzur
openaire +3 more sources
Protein kinase C‐dependent stimulation of phospholipase D in phospholipase C‐treated fibroblasts
Lipids, 1993AbstractTreatment of [14C]choline‐ or [14C]ethanolamine‐labeled NIH 3T3 fibroblasts withBacillus cereus phosphatidylcholine‐specific phospholipase C (PLC) enhanced phospholipase D (PLD)‐mediated hydrolysis of the respective14C‐labeled phospholipids. PLD activity was stimulated by 1.5 U/mL of POLC and by 100 nM of the protein kinase C (PKC) activator ...
Zoltan Kiss, Nandor Garamszegi
openaire +3 more sources
[49] Properties of phospholipase C isozymes
1991Publisher Summary Phospholipase C (PLC)-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) has been proposed to be a crucial step in the cellular response to calcium-mobilizing hormones and neurotransmitters. Numerous attempts to isolate PLC from various tissues have been made and as a result, various kinds of PLC have been ...
Yasufumi Emori +2 more
openaire +3 more sources
Primary phospholipase C and brain disorders
Advances in Biological Regulation, 2016In the brain, the primary phospholipase C (PLC) proteins, PLCβ, and PLCγ, are activated primarily by neurotransmitters, neurotrophic factors, and hormones through G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs). Among the primary PLC isozymes, PLCβ1, PLCβ4, and PLCγ1 are highly expressed and differentially distributed ...
Yang, Yong Ryoul +6 more
openaire +4 more sources