Results 211 to 220 of about 117,202 (259)
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The transphosphatidylation activity of phospholipase D
Molecular and Cellular Biochemistry, 1996Transphosphatidylation activity is a characteristic and remarkable property of phospholipase D (PLD) and has been studied in plants and mammalian tissues. This reaction is often used to confirm the properties and/or abnormalities of PLD activity. The mechanism for activating PLD transphosphatidylation seems multiple.
C H, Yu, S Y, Liu, V, Panagia
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Dimerization of Phospholipase D Isozymes
Biochemical and Biophysical Research Communications, 2002Two mammalian phospholipase D (PLD) isozymes (PLD1 and PLD2) have been reported. In this study, we differentially tagged these isozymes with enhanced green fluorescent protein (EGFP-rPLD1 and EGFP-rPLD2) or Xpress peptide epitope (Xpress-rPLD1 and Xpress-rPLD2) to examine the association between these isozymes.
Yoonseok, Kam, John H, Exton
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Isolation of an Activator for Phospholipase D
Nature, 1959WHILE working on the purification of phospholipase D for use in connexion with other studies on phos-pholipids1 we observed that incubation with the enzyme released choline from commercial soy bean lecithin, but not from synthetic L-α-dipalmitoyl lecithin or carefully purified lecithins from egg, yeast and soy beans.
H, WEISS, H E, SPIEGEL, E, TITUS
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Phospholipase D structure and regulation
Chemistry and Physics of Lipids, 1999The recent identification of cDNA clones for phospholipase D has opened the door to new types of investigations into its structure and regulation. PLD activity has been found to be encoded by at least two different genes that contain catalytic domains that relate their mechanism of action to phosphodiesterases. In vivo roles for PLD suggest that it may
M A, Frohman, A J, Morris
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Phospholipase D and cell signaling
Current Opinion in Immunology, 1993Phospholipase D, which hydrolyzes phospholipids (primarily phosphatidylcholine) to generate phosphatidic acid, has emerged as a critical component in cellular signal transduction. Research during the past year has confirmed and extended the view that phosphatidic acid and its dephosphorylated product, sn-1,2-diacylglycerol, are important intracellular ...
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Measurement of Phospholipase D Activity
Analytical Biochemistry, 1997Phosphodiesteric cleavage of phosphatidylcholine by members of a growing family of phospholipases D produces choline and phosphatidic acid. These enzymes can also catalyse a transphosphatidylation reaction in which the aliphatic chain of a primary alcohol is transferred to the phosphatidyl moiety of the phosphatidic acid product.
A J, Morris, M A, Frohman, J, Engebrecht
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The Preparation of Phospholipids by Phospholipase D
1978The transfer of the phosphatidyl residue from egg phosphatidylcholine to primary alkanols catalyzed by phospholipase D was systematically investigated. It was demonstrated that 1) the chain length of the alkanols is of critical importance, e.g. transphosphatidylation does not occur to alkanols or alkandiols with more than six carbon atoms; 2) double or
S, Kovatchev, H, Eibl
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Biochemical Analysis of Phospholipase D
2007Phospholipase D (PLD) is distributed widely in nature, being present in various isoforms in bacteria, protozoa, fungi, plants, and animals. It catalyzes the hydrolysis of phospholipids, primarily phosphatidylcholine (PC), into phosphatidic acid (PA) and the head group, choline.
H Alex, Brown +4 more
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