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Cancer Science, Volume 117, Issue S1, Page 1-2148, January 2026.
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Mitochondrial phosphoprotein metabolism

Biochimica et Biophysica Acta, 1963
Abstract 1. 1. Rat-liver mitochondria incubated at 20° in a medium of KCl, Tris, Versene, and containing 32 P, rapidly take up the tracer into ATP and phosphotein fractions. Phospholipids are not labelled under these conditions. 2. 2. Inhibitors of electron transport and of oxidative phosphorylation reduce the rate of labelling of the ...
K, AHMED, J D, JUDAH
openaire   +2 more sources

Phosphoprotein Staining with the GelCode Phosphoprotein Staining Kit

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThe phosphorylation state of a protein has an important role in the regulation of a wide variety of cellular processes. As a result, there has been a great deal of interest in detecting phosphorylated proteins. The method presented here uses the GelCode phosphoprotein staining kit (Pierce Chemical Company).
Wayne R, Stochaj   +2 more
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Isolation of Phosphoproteins

2008
Phosphorylation is one of the most abundant post-translational modifications on protein and one that frequently has functional biological consequences. For this reason, screening protein samples for phosphorylations has become an important tool in biochemical research.
Lawrence G, Puente, Lynn A, Megeney
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Immunohistochemical Techniques for Phosphoproteins

2022
The use of immunohistochemical techniques to study the patterns of protein phosphorylation has revolutionized the study of signaling pathways. This technique allows detecting the phosphorylated state of signaling proteins in formalin-fixed and paraffin-embedded tissue sections by using phosphospecific antibodies.
Berenice L, Sanz Ressel   +1 more
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Chloroplast Phosphoproteins. Evidence for a Thylakoid‐Bound Phosphoprotein Phosphatase

European Journal of Biochemistry, 1980
Isolated intact pea (Pisum sativum) chloroplasts incorporate [32P]orthophosphate into several thylakoid polypeptides in the light. Transfer of the labelled chloroplasts to darkness results in rapid dephosphorylation of the polypeptides. The most rapidly dephosphorylated phosphoproteins are the 26000‐Mr doublet derived from the light‐harvesting ...
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Sodium Transport and Phosphoproteins

Nature, 1962
IN previous work1 we have shown that liver slices suspended in a medium free of sodium ions show a depressed turn-over of phosphoprotein phosphorus. This effect is reversed by addition of sodium. In these and the experiments shown here, the phosphoproteins have been identified by isolation of radioactive pbosphorylserine from acid hydrolysates of the ...
J D, JUDAH, K, AHMED
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Phosphoproteins and Sodium Transport

Nature, 1962
A FAIR measure of agreement has been reached that active transport of ions and volume regulation of cells involves utilization of adenosine triphosphate (ATP). We have put forward the suggestion that the phosphorylation of phosphoprotein is the first step in this utilization of ATP.
J D, JUDAH, K, AHMED, A E, McLEAN
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Chromatin phosphatides and phosphoprotein

Life Sciences, 1969
Abstract Phospholipids of chromatin isolated from rat liver were studied. The comparable ratios between phospholipid, DNA and protein were investigated. Individual phosphatide in the lipid fraction was identified and estimated by the combined techniques of column, thin-layer, and paper chromatography.
M, Song, M, Ledig, P, Mandel
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Analysis of plant phosphoproteins

Analytical Biochemistry, 2007
Chromatography supports to purify phosphorylated proteins (P-proteins) have become available recently, yet this has not been thoroughly investigated in the case of plant materials. In this study we used a commercial affinity matrix (Qiagen) and a test plant enzyme (phosphoenolpyruvate carboxylase PEPC).
P, Meimoun   +4 more
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