Results 201 to 210 of about 26,651 (230)
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A spectrophotometric assay for adenine phosphoribosyltransferase
Analytical Biochemistry, 1977Abstract The present paper describes a new spectrophotometric assay for adenine phosphoribosyltransferase activity which is highly reproducible, rapid, sensitive, and simpler than the isotopic assays for this enzyme. This assay is based on the quantitative measurement of the product AMP by a NADH-coupled enzyme method.
SALERNO, Costantino +1 more
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Transformation of the gene for hypoxanthine phosphoribosyltransferase [PDF]
Somatic Cell Genetics, 1979 Purified DNA from wild-type Chinese ovary (CHO) cells has been used to transform three hypoxanthine phosphoribosyltransferase (HPRT) deficient murine cell mutants to the enzyme positive state. Transformants appeared at an overall frequency of 5 x 10(-8) colonies/treated cell and expressed CHO HPRT activity as determined by electrophoresis.
Lawrence A. Chasin +2 more
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Rapid assay for purine phosphoribosyltransferases
Analytical Biochemistry, 1972Abstract A micro assay for purine phosphoribosyltransferases has been developed using small Dowex 1-X8 columns. This method compares favorably in accuracy, sensitivity, and reproducibility with many established procedures. It seems to be most suitable for enzyme purifications, which require many assays to be performed in a reasonable time.
Wolf Gutensohn, Gordon Guroff
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Facilitated Purification of Hypoxanthine Phosphoribosyltransferase
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1976Three major approaches to the complete purification of hypoxanthine phosphoribosyltransferase from human erythrocytes and rat brain are described. Preparative isoelectric focusing which has been used for the isolation of the human enzyme was not fully successful in the case of rat brain.
Marianne Huber +2 more
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Hysteretic characteristic of adenine phosphoribosyltransferase
Biochemistry, 1975Preassay-incubation of the highly purified human erythrocyte adenine phosphoribosyltransferase (EC 2.4.2.7) (AMP pyrophosphorylase) with one of its substrates, 5-phosphoribosyl 1-pyrophosphate (PRibPP), changes the apparent V max value of the enzyme reaction.
Balis Me, Yip Lc
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1974
Partial deficiency of HGPRT, a salvage enzyme of purine metabolism, has been demonstrated to be the primary abnormality causing purine overproduction in a small proportion of patients with gout (1–4). The quantitative deviation in the activity of this enzyme has been shown by Kelley et al.
A. de Vries, Oded Sperling, P. Boer
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Partial deficiency of HGPRT, a salvage enzyme of purine metabolism, has been demonstrated to be the primary abnormality causing purine overproduction in a small proportion of patients with gout (1–4). The quantitative deviation in the activity of this enzyme has been shown by Kelley et al.
A. de Vries, Oded Sperling, P. Boer
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Adenine Phosphoribosyltransferase Deficiency in Iceland
Acta Medica Scandinavica, 1988Abstract. Two children and two adults of four unrelated families were on regular light microscopic examination found to exhibit identical, spherical urine crystals. Their characteristic appearance led to the diagnosis of 2,8‐dihydroxyadenine crystalluria by spectrophotometric or gas‐chromatographic/mass‐spectrometric analysis.
Tómas Á. Jónasson, Thröstur Laxdal
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The Purine Phosphoribosyltransferases of Crithidia fasciculata
The Journal of Parasitology, 1979The purine phosphoribosyltransferases of Crithidia fasciculata were identified and some of their properties described. The organism possesses three separate enzymes for the production of AMP, IMP, and GMP. The evidence for this comes from the observed differences in elution patterns from gel filtration columns, differences in heat sensitivity, and ...
George W. Kidder +2 more
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Characterization of an adenine phosphoribosyltransferase deficiency
Clinica Chimica Acta, 1988A case of adenine phosphoribosyltransferase deficiency in a 4.5-yr-old boy is described. A pedigree of the family, enzyme activities and kinetic data of the enzyme in the propositus and the carriers of the defect are presented. The amount of enzyme in the patient was about 2% of that in healthy subjects and correlated well with the amount of ...
K. Zwiauer, P. Chiba, Mathias Müller
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