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Evolutionary analysis of Trehalose breakdown pathways

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Ganesh Muthu G   +2 more
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From Phosphorylase to Phosphorylase Kinase

Advances in second messenger and phosphoprotein research, 1997
Glycogen phosphorylase was the first protein shown to be controlled by reversible phosphorylation (Fischer and Krebs 1955). Control by protein phosphorylation is now known to be a ubiquitous process for intracellular signalling mechanisms. There is a growing number of protein kinase and protein phosphatase structures solved by X-ray diffraction methods
L N, Johnson   +4 more
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Lymphocyte phosphorylase kinase

International Journal of Biochemistry, 1981
Abstract 1. 1. Phosphorylase kinase from pig lymphocytes has been purified 275-fold. 2. 2. The molecular weight of the purified enzyme was found to be 200,000. 3. 3. Optimum pH of the enzyme was dependent on ATP-Mg 2+ concentration. 4. 4. Maximum activity was obtained at equivalent molar concentration of ATP and Mg 2+ . 5.
F, Javier Gella   +2 more
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The phosphorylase kinase activity of hearts from phosphorylase kinase-deficient mice

Biochemical Genetics, 1983
In an assay measuring radioactive incorporation from gamma--P32P]ATP into phosphorylase b, cardiac muscle extracts from mice with the phosphorylase kinase deficiency mutation showed significant, calcium-dependent phosphorylase kinase activity that was 10 to 15% of that of Swiss mice, the control strain.
S R, Gross, J J, Connor, R M, Johnson
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Phosphorylase kinase deficiency

Biochemical Genetics, 1970
Phosphorylase kinase deficiency in I strain mice and in humans both show X-chromosomal inheritance. Neither deficient adult humans nor deficient mice show any sign of disease. Thus the two conditions resemble each other. However, there are differences.
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The crosslinking of phosphorylase kinase

Archives of Biochemistry and Biophysics, 1982
Abstract Phosphorylase kinase from rabbit skeletal muscle has been crosslinked with dimethylsuberimidate and the products have been examined by SDS-gel electrophoresis. Identification of crosslinked species was made from their molecular weights and from their susceptibility to digestion by specific proteolytic enzymes.
P K, Lambooy, R F, Steiner
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