Results 131 to 140 of about 62,445 (296)

Fluorescence and Photobleaching in Photodynamic Therapy [PDF]

, 1997
The phenomenon of photobleaching of a photosensitizer during photodynamic therapy (PDT) is well known. For second generation photosensitizers it may be possible to exploit this effect to enhance the volume of damaged tissue and improve the efficacy of ...
Hawkes, Robert
core  

Detection of PCNA ubiquitination by acceptor photobleaching FRET.

, 2013
A. A usp1 DT40 cell before and after bleaching of the mRFP acceptor. B. Whole cell spectrum, of the cell shown in A, with excitation at 515 nm (Ex515/ΣλmRFP) before (black line) and after (grey line) photobleaching. C. Whole cell spectrum with excitation
Christopher Batters (367446), Julian E. Sale (7697), Hannah Zhu (367447)   +2 more
core   +1 more source

Fine‐Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity

Advanced Science, EarlyView.
STAU2 undergoes phase separation to form dynamic condensates that package target mRNAs and deliver them to the distal ends of growing neuronal dendrites. STAU2 condensates stabilize embedded mRNAs and repress their translation. Synaptic activity bidirectionally remodels STAU2 condensates, coordinating local translation of STAU2‐associated mRNAs ...
Shijing Huang, Yuanyuan Chu, Ruiqian Zhao, Siyu Li, Yu Feng, Hongdan Zheng, Yunli Xie, Tong Wang, Wenyu Wen   +8 more
wiley   +1 more source

Cell images from standard acceptor photobleaching FRET.

, 2013
(A–C) Cos-1 cells transfected with plasmids encoding the CFP-YFP fusion proteins as indicated. (D) Representative cells transfected with empty vectors as a negative control for FRET.
Qing Zhang (1802), Yuan-Bin Zheng (293698), Peilin Xu (293703), Peng Tan (293702), Ying-Ying Xiao (293700)   +4 more
core   +1 more source

Bidirectional Photoswitching of a Tailored Azobenzene with Red and Far‐Red Light Involving Triplet Sensitization in an Aqueous System

Advanced Science, EarlyView.
The action spectrum of a tailored azobenzene is shifted toward bio‐optical window through the chemical modification of its singlet and triplet energy levels. Consequently, the bidirectional photo‐switching of azobenzene in physiological conditions is achieved when excited by red and far‐red light, which offers greater tissue penetration.
Mila Miroshnichenko, Helen Hölzel, Edvinas Orentas, Karolis Kazlauskas, Pedro Ferreira, Fabienne Dumoulin, Carles Alcaide, Miquel Solà, Roger Bresolí‐Obach, Santi Nonell, Pankaj Bharmoria, Kasper Moth‐Poulsen   +11 more
wiley   +1 more source

Photobleaching of Fluorophores on the Surface of Nanoantennas

, 2016
Photobleaching is a widespread problem in any optical study or application involving fluorophores and is particularly acute in the context of single-molecule detection.
J. Grand (1303554), E. C. Le Ru (1706398), C. Artur (1706392), C. M. Galloway (1706395)   +3 more
core   +1 more source

A Correlative SICM‐OPM Platform for Surface and Volumetric Imaging in Live Cells

Advanced Science, EarlyView.
A multifunctional correlative imaging platform integrating Scanning Ion Conductance Microscopy (SICM) with Oblique Plane Microscopy (OPM) enables simultaneous surface topography, mechanical mapping, and 3D volumetric fluorescence imaging in live cells.
Wenzhi Hong, Ziwei Zhang, Ao Li, Ting Sun, Yunzhao Wu, Devkee M. Vadukul, Dylan Jones, Bing Li, Fengjie Liu, Francesco A. Aprile, Yuri Korchev, Julia Gorelik, David Klenerman, Andrew Shevchuk   +13 more
wiley   +1 more source

DNA Nanoarray for Multiplexed RNA Detection With Single‐Molecule Readout

Advanced Science, EarlyView.
A carrier‐based DNA nanoarray integrates programmable nucleic acid hybridization with solid‐state nanopore readout for direct RNA detection at single‐molecule resolution. A ternary encoding system enables 27 carriers with 81 addressable sensing sites, allowing the simultaneous identification of targets spanning a wide size range, from short microRNAs ...
Yunxuan Li, Yesheng Wang, Chunmiao Yu, Siyu Hou, Elli Mylona, Plamena Naydenova, Jingyuan Zhao, Hong Yuan, Jingsong Duan, Hendrik F. P. Runge, Stephen Baker, Jinbo Zhu, Ulrich F. Keyser   +12 more
wiley   +1 more source

Chlorography or Chlorotyping from the Decomposition of Chlorophyll and Natural Pigments in Leaves and Flowers as a Natural Alternative for Photographic Development

Journal of Zoological and Botanical Gardens
This study explores the use of chlorography as a natural photographic developing technique that utilizes the decomposition of chlorophyll and other plant pigments through the action of sunlight.
Andrea D. Larrea Solórzano, Iván P. Álvarez Lizano, Pablo R. Morales Fiallos, Carolina E. Maldonado Cherrez, Carlos S. Suárez Naranjo   +4 more
doaj   +1 more source

Compact fluorescence system with fiber-coupled LED for studying photobleaching

, 2018
[[abstract]]Laser-induced fluorescence systems that are used in fluorescence research are expensive. This study demonstrated a novel, cheap, and compact fluorescence system comprising a fiber-coupled LED and pinholes to achieve a confocal arrangement ...
Weng, CJ;Lien, CH;Chen, CY;Yuh, CH;Chen, CJ;Lin, YS
core   +1 more source