Results 51 to 60 of about 4,764 (184)

Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts. [PDF]

PLoS ONE, 2014
The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells.
Boyu Cui, Lifeng Zhang, Yunhong Song, Jinsong Wei, Changfu Li, Tietao Wang, Yao Wang, Tianyong Zhao, Xihui Shen   +8 more
doaj   +1 more source

Comparative Genomics and Pathogenicity Analysis of Two Bacterial Symbionts of Entomopathogenic Nematodes: The Role of the GroEL Protein in Virulence

Microorganisms, 2022
Bacteria of the genera Xenorhabdus and Photorhabdus are symbionts of entomopathogenic nematodes. Despite their close phylogenetic relationship, they show differences in their pathogenicity and virulence mechanisms in target insects.
Abraham Rivera-Ramírez, Rosalba Salgado-Morales, Alfredo Jiménez-Pérez, Rebeca Pérez-Martínez, Blanca Inés García-Gómez, Edgar Dantán-González   +5 more
doaj   +1 more source

A Spontaneous Mutation in tfoX Impairs Bioluminescence in Photobacterium kishitanii ANT‐2200

Molecular Microbiology, EarlyView.
A frameshift mutation in tfoX generates a truncated regulator associated with reduced lux–rib expression and low luminescence. Complementation with wild‐type tfoX restores transcription and light production, supporting a role for TfoX in regulating bioluminescence independently of quorum sensing.
Capucine Le Cam Ligier, Gwenola Simon, Charlotte Berthelier, Corinne Valette, Elisa Lefeuvre, Marc Garel, Laurie Casalot   +6 more
wiley   +1 more source

Biosynthese des vollständig gesättigten C16‐Homosesquiterpens Descartan durch Deprotonierung mit Cyclopropanierung

Angewandte Chemie, Volume 138, Issue 38, 14 September 2026.
Eine nicht‐kanonische C16‐Sesquiterpensynthase aus Morganella morganii produziert das ungewöhnliche Grundgerüst Descartan. Ihr Cyclisierungsmechanismus wurde mittels Isotopenmarkierungsexperimenten und DFT‐Berechnungen aufgeklärt. Gezielte Mutagenese veränderte die Enzymfunktion und führte zu alternativen Produkten, darunter Voltairen, dessen ...
Kexin Yang, Philip Troycke, Bernd Goldfuss, Michael Groll, Jeroen S. Dickschat   +4 more
wiley   +1 more source

The Type VI secretion systems of the insect pathogen Photorhabdus luminescens are involved in interbacterial competition, motility and secondary metabolism

The Microbe
The Type VI Secretion System (T6SS) is used as weapon by a variety of Gram-negative bacteria in polymicrobial niche competition. Its characterization and study gained more interest in recent years.
Friederike Pisarz, Timo Glatter, Dhana-Theresa M. Süss, Ralf Heermann, Alice Regaiolo   +4 more
doaj   +1 more source

A novel tool for stable genomic reporter gene integration to analyze heterogeneity in Photorhabdus luminescens at the single-cell level

BioTechniques, 2015
Determination of reporter gene activity at the single-cell level is a prerequisite for analyzing heterogeneous gene expression in bacteria. The insect pathogenic enteric bacterium Photorhabdus luminescens is an excellent organism in which to study ...
Angela Glaeser, Ralf Heermann
doaj   +1 more source

Biosynthesis of the Fully Saturated C16 Homosesquiterpene Descartane Through Deprotonation With Cyclopropanation

Angewandte Chemie International Edition, Volume 65, Issue 38, 14 September 2026.
A non‐canonical C16 sesquiterpene synthase from Morganella morganii produces the unusual scaffold descartane. Its cyclisation mechanism was rationalised by isotopic labelling experiments and DFT calculations. Site‐directed mutagenesis altered enzyme function, resulting in alternative products, including voltairene, which requires a different ...
Kexin Yang, Philip Troycke, Bernd Goldfuss, Michael Groll, Jeroen S. Dickschat   +4 more
wiley   +1 more source

A bacterial binary toxin system that kills both insects and aquatic crustaceans: Photorhabdus insect-related toxins A and B.

PLoS Pathogens, 2023
Photorhabdus insect-related toxins A and B (PirA and PirB) were first recognized as insecticidal toxins from Photorhabdus luminescens. However, subsequent studies showed that their homologs from Vibrio parahaemolyticus also play critical roles in the ...
Hao-Ching Wang, Shin-Jen Lin, Han-Ching Wang, Ramya Kumar, Phuoc Thien Le, Jiann-Horng Leu   +5 more
doaj   +1 more source

Advances in large DNA fragment assembly for microbial cell factory engineering

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang, Wenqian Liu, Dongyuan Cheng, Jiazhang Lian, Xian Fu, Yue Shen   +5 more
wiley   +1 more source

Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts.

PLoS ONE, 2019
Photorhabdus luminescens is an entomopathogenic bacterium found in symbiosis with the nematode Heterorhabditis. Dam DNA methylation is involved in the pathogenicity of many bacteria, including P.
Amaury Payelleville, Dana Blackburn, Anne Lanois, Sylvie Pagès, Marine C Cambon, Nadege Ginibre, David J Clarke, Alain Givaudan, Julien Brillard   +8 more
doaj   +1 more source