Results 101 to 110 of about 1,929,062 (267)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Exercise‐induced changes in left ventricular strain are affected by interleukin‐6 activity: An exploratory analysis of a randomised‐controlled trial in humans with abdominal obesity

Experimental Physiology
Whilst the exercise‐induced myokine interleukin‐6 (IL‐6) plays a beneficial role in cardiac structural adaptations, its influence on exercise‐induced functional cardiac outcomes remains unknown. We hypothesised that IL‐6 activity is required for exercise‐
Simon Jønck, Mathilde Løk, Cody Durrer, Anne‐Sophie Wedell‐Neergaard, Louise Lang Lehrskov, Grit Elster Legaard, Rikke Krogh‐Madsen, Jaya Rosenmeier, Morten Asp Vonsild Lund, Bente Klarlund Pedersen, Helga Ellingsgaard, Ronan M. G. Berg, Regitse Højgaard Christensen   +12 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The environment and physical activity.

, 2014
Until recently, obesity research has mainly focused on biological and behavioral factors. However, there is growing agreement among researchers that the social and physical environment may play an important role as well. Certain aspects of the environment may promote or discourage physical activity and therefore have an influence on children’s body ...
D'Haese, Sara, Cardon, Greet, Deforche, Benedicte   +2 more
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Exploring sedentary behaviour reduction interventions in the primary healthcare setting: a scoping review

BMC Primary Care
Background Excessive sitting time is a risk factor for many non-communicable diseases and an increased risk of early mortality. Primary healthcare is a viable setting to target population health behaviour change.
Aaron Beecroft, Anna Chapman, Shannon Sahlqvist, Lisa Becker, David Dunstan   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Cluster analysis of physical activity and physical fitness and their associations with components of school skills in children aged 8–9 years

Scientific Reports
This study aimed to cluster physical activity (PA), physical fitness, visual-motor coordination, household duties, and their associations with school skills in 170 primary school children (88 girls and 82 boys) aged 8–9 years.
Agata Korcz, Łukasz Bojkowski, Michał Bronikowski, Marlena Łopatka, Maryna Khorkova, Agnieszka Koszałka-Silska, Elżbieta Cieśla, Jana Krzysztoszek   +7 more
doaj   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source