Results 91 to 100 of about 1,360,407 (261)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Urban Therapy-Urban Health Path as an Innovative Urban Function to Strengthen the Psycho-Physical Condition of the Elderly. [PDF]

Int J Environ Res Public Health, 2023
Szewczenko A, Lach E, Bursiewicz N, Chuchnowska I, Widzisz-Pronobis S, Sanigórska M, Elsner K, Bal D, Sutor M, Włodarz J, Ober J.   +10 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Absence and the Physical Conditions of Work [PDF]

Occupational and Environmental Medicine, 1957
R D, SHEPHERD, J, WALKER
openaire   +2 more sources

Physical conditions in the Homunculus

, 2005
9 pages, 3 figs, ASP Conf ...
Ferland, Gary J., Abel, Nick, Davidson, Kris, Smith, Nathan   +3 more
openaire   +2 more sources

Evolution of the internal load and physical condition of wheelchair basketball players during the competitive season. [PDF]

Front Physiol, 2023
Romarate A, Yanci J, Iturricastillo A.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Proposal for a Battery to Evaluate Functional Capacity in Older Adults with Disabilities

Sensors
The purpose was to design and validate a battery of physical tests, called EFEPD-1.0, adapted to assess functionality in people with disabilities. In addition, we sought to analyze the validity and reliability of this battery both for the total group and
Josu Ascondo, Iñaki Llodio, Bingen Marcos-Rivero, Cristina Granados, Sheila Romero, Aitor Iturricastillo, Javier Yanci   +6 more
doaj   +1 more source

The Problem of Monitoring the Psycho-Physical Condition of Seniors during Proposed Activities in Urban Space. [PDF]

Sensors (Basel), 2023
Lach E, Szewczenko A, Chuchnowska I, Bursiewicz N, Benek I, Widzisz-Pronobis S, Bal D, Elsner K, Sanigórska M, Sutor M, Włodarz J.   +10 more
europepmc   +1 more source