Results 121 to 130 of about 28,137,191 (312)

Physical modelling of water, fauna and flora: knowledge gaps, avenues for future research and infrastructural needs

, 2014
Physical modelling is a key tool for generating understanding of the complex interactions between aquatic organisms and hydraulics, which is important for management of aquatic environments under environmental change and our ability to exploit ecosystem ...
Robert E. Thomas, Iahr Member, Matthew F. Johnsonb, L. Frostick, Daniel R. Parsonsa, T. J. Boumac, Jasper T. Dijkstrad, Olivier Eiffe, Sylvie Gobertf, Pierre-Yves Henryg, Paul Kemph, Stuart J. Mclellanda, F. Moulin, Dag Myrhaugg, Alexandra Neytsg, M. Pauli, W. E. Penningd, Sara Puijalonj, Stephen P. Riceb, Adrian Stanicak, Davide Tagliapietral, Michal Talm, Alf Tørumn, Michalis I. Vousdoukasio, S. Puijalon, S. Rice, A. Stanica, D. Tagliapietra, A. Tørum   +28 more
semanticscholar   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Physical modelling of a surface-wave survey over a laterally varying granular medium with property contrasts and velocity gradients

, 2014
Laboratory experiments using laser-based ultrasonic techniques can be used to simulate seismic surveys on highly controlled small-scale physical models of the subsurface. Most of the time, such models consist in assemblies of homogeneous and consolidated
P. Bergamo, L. Bodet, L. Socco, R. Mourgues, Vincent Tournat   +4 more
semanticscholar   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Density Functional Theory Study of Ethylene Carbonate Adsorption on the (0001) Surface of Aluminum Oxide α‑Al2O3

ACS Omega, 2021
Brian Ramogayana, David Santos-Carballal, Khomotso P. Maenetja, Nora H. de Leeuw, Phuti E. Ngoepe   +4 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Validation of an LNG Ship Added-Resistance Prediction Framework Using Onboard Measured Data

Journal of Marine Science and Engineering
This study sets and evaluates a practical framework for predicting ship resistance under real operational conditions along a global shipping route. Despite extensive research, the literature lacks straightforward studies that separately assess wind, wave,
Ante Čalić, Nur Assani, Goran Rilje, Marko Katalinić   +3 more
doaj   +1 more source