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Freshman Year Computer Engineering Students' Experiences For Flipped Physics Lab Class: An Action Research

open access: yes, 2017
The purpose of this research is to determine the university students' learning experiences about flipped-physics laboratory class. The research has been completed during the fall semester of 2015 at Computer Engineering Department of Istanbul ...
GÜREL, ZEYNEP
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Complexity, Fractality and Fractional Dynamics Applied to Science and Engineering

open access: yesFractal and Fractional
Many problems in classical and quantum physics, statistical physics, engineering, biology, psychology, economics, and finance are inherently global rather than merely local, often exhibiting long-range correlations in time and space, memory effects ...
Alexandra M. S. F. Galhano   +2 more
doaj   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Un modelo integrador para le Enseñanza de la Física en la carrera de Ingeniería Agronómica

open access: yesRevista de Enseñanza de la Física, 2016
This work is immersed within a bigger proposal: The Research and Practice Integration within the Physics Teaching. Its aim is to integrate Physics teachers and Teaching Physics investigators into the drawing out of curricula aimed to overcome detected ...
Mónica Moya de Ovando   +1 more
doaj  

Considering the Impact of Using Free-Body Diagram in Mechanics Problem-Solving on Improving Engineering Students’ Performance in Physics at a Selected Public University in Rwanda

open access: yesJournal of Learning Theory and Methodology
Background. Engineering students often struggle with physics problem-solving, especially mechanics problems, while teaching mechanics helps students predict motion and force impacts, enabling creative engineering design tasks.
Theogene Niyomufasha   +2 more
doaj   +1 more source

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