Results 61 to 70 of about 103,645 (313)

Effect of pooled tracheal sample testing on the probability of Mycoplasma hyopneumoniae detection

open access: yesScientific Reports
Tracheal pooling for Mycoplasma hyopneumoniae (M. hyopneumoniae) DNA detection allows for decreased diagnostic cost, one of the main constraints in surveillance programs.
Ana Paula Serafini Poeta Silva   +13 more
doaj   +1 more source

Assessment of Genetic Diversity among Pigeon pea Male Sterile lines and Popular Cultivars using SSR Markers

open access: yesElectronic Journal of Plant Breeding, 2016
In the present study genetic diversity studies have been carried out using twenty five SSR markers in a set of pigeonpea genotypes comprising of male sterile lines and popular cultivars. The primers amplified a total of 99 bands with highest PIC of 0.79.
SNCVL Pushpavalli   +2 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

PIC BASIC projects : 30 projects using PIC BASIC and PIC BASIC PRO / [PDF]

open access: yes
A wealth of simple and advanced projects to show how PIC BASIC can be used to program the most widely used microcontroller on the market.Includes index.Print version record.Front Cover; PIC BASIC Projects: 30 Projects Using PIC BASIC and PIC BASIC PRO ...
Ibrahim, Dogan.
core  

[Théâtre Nô : photographies / Roger Pic] [PDF]

open access: yes, 1957
Appartient à l’ensemble documentaire ...
Pic, Roger (1920-2001). Photographe
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

open access: yesFEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

[Danses d'Allemagne : photographies / Roger Pic] [PDF]

open access: yes, 1963
Appartient à l’ensemble documentaire ...
Pic, Roger (1920-2001). Photographe
core  

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

open access: yesFEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey   +4 more
wiley   +1 more source

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