Results 91 to 100 of about 70,875 (272)

Preparation of Pichia pastoris Expression Plasmids [PDF]

, 2012
When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, C, Alkhalfioui, F, Byrne, B, Wagner, R   +3 more
openaire   +4 more sources

Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microbial enzymes expressed in plants add new functionalities but occasionally trigger undesirable immune responses. Phanerochaete carnosa glucuronoyl esterase (PcGCE) hydrolyses the bond between lignin and 4‐O‐methyl‐α‐D‐glucuronic acid substituent of glucuronoxylan.
Evgeniy N. Donev, Marta Derba‐Maceluch, Xiao‐Kun Liu, Henri Colyn Bwanika, Izabela Dobrowolska, Mohit Thapa, Joanna Leśniewska, Jan Šimura, Alex Yi‐Lin Tsai, Konrad S. Krajewski, Dan Boström, Leszek A. Kleczkowski, Maria E. Eriksson, Karin Ljung, Emma R. Master, Ewa J. Mellerowicz   +15 more
wiley   +1 more source

Nonequilibrium roughening transition in a simple model of fungal growth in 1+1 dimensions

, 1998
We introduce a simple model of yeast-like growth of fungi colonies, which exhibits a continuous roughening transition far from equilibrium from a smooth ($\alpha = 0$) to rough phase ($\alpha = 1/2$) in 1+1 dimensions.
Jensen, Henrik Jeldtoft, Lopez, Juan M.
core   +1 more source

Uncovering Convergent Pattern Recognition Receptors Recognising Phytophthora Across Plant Lineages

Plant Biotechnology Journal, EarlyView.
ABSTRACT Pattern recognition receptors (PRRs) are pivotal for plant immunity, yet their discovery in crops is hindered by lineage‐specific divergence. We demonstrate that microbe‐associated molecular patterns (MAMPs) often activate immunity through phylogenetically unrelated, convergently evolved PRRs across plant lineages.
Yong Pei, Yaning Zhao, Hui Wang, Yining Guo, Xinyi Gu, Jingkun Lv, Zhenjie Guo, Yanjun Chen, Yingkai Ren, Yanrong Ren, Jianyu Yan, Yuke Wang, Peiyun Ji, Danyu Shen, Zhiyuan Yin, Daolong Dou   +15 more
wiley   +1 more source

Molecular Characterization of Fermenting Yeast Species from Fermented Teff Dough during Preparation of Injera Using ITS DNA Sequence

International Journal of Food Science, 2019
Identification of the yeast responsible for Injera fermentation is important in order to be more consistent and for scale-up of Injera production.
Belay Tilahun Tadesse, Andualem Bahiru Abera, Anteneh Tesfaye Tefera, Diriba Muleta, Zewdu Terefework Alemu, Gary Wessel   +5 more
doaj   +1 more source

Technologies for restricting mould growth on baled silage [PDF]

, 2007
End of project reportSilage is made on approximately 86% of Irish farms, and 85% of these make some baled silage. Baled silage is particularly important as the primary silage making, storage and feeding system on many beef and smaller sized farms, but is
Clipson, Nicholas J.W., Doohan, Fiona, Doyle, Evelyn M., Egan, Damian, Forristal, Dermot, Frisvad, Jens C., Fuller, Hubert T., Laffin, Christopher, McEniry, Joseph, McNally, Gerard M., Nielsen, Kristian F., O'Brien, Martin, O'Kiely, Padraig, Small, Christopher M.   +13 more
core  

Effects of Exogenous Yeast and Bacteria on the Microbial Population Dynamics and Outcomes of Olive Fermentations. [PDF]

, 2017
In this study, we examined Sicilian-style green olive fermentations upon the addition of Saccharomyces cerevisiae UCDFST 09-448 and/or Pichia kudriazevii UCDFST09-427 or the lactic acid bacteria (LAB) Lactobacillus plantarum AJ11R and Leuconostoc ...
Charlotte Tyler, Elaine Chow, Jose Zaragoza, Kyria Boundy-Mills, Maria L. Marco, Mary E. Kable, Thomas R. Williams, Timothy M. LaPara, Yelizaveta Luchkovska, Zachary Bendiks   +9 more
core   +2 more sources

Engineering of 10‐Deacetylbaccatin III‐10‐β‐O‐Acetyltransferase From Taxus Species for Efficient Acetylating Non‐Natural Substrates Into Taxol in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT 7‐β‐xylosyl‐10‐deacetyltaxol (XDT) is much more abundant than the anticancer drug Taxol in Taxus species and is usually regarded as the by‐product of Taxol. It could be enzymatically transformed into 10‐deacetyltaxol (DT), and the latter could be further converted into Taxol.
Tian‐Jiao Chen, Jing‐Jing Chen, Xiao‐Yan Sun, Ting Gong, Jin‐Ling Yang, Ping Zhu   +5 more
wiley   +1 more source

Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes

VacciMonitor, 2021
La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez, Lourdes Zumalacárregui-de-Cárdenas, Zeila Santana-Vázquez   +2 more
doaj  

Structure, Mechanism, and Specificity of a Eukaryal tRNA Restriction Enzyme Involved in Self-Nonself Discrimination

Cell Reports, 2014
Summary: tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death.
Anupam K. Chakravarty, Paul Smith, Radhika Jalan, Stewart Shuman   +3 more
doaj   +1 more source