Results 191 to 200 of about 34,603 (231)

Glycosylation in Pichia pastoris

Biotechnology and Applied Biochemistry, 1999
The Pichia pastoris system for expression of heterologous recombinant proteins is being used increasingly because of the large yields of properly folded proteins that result and the ease of scaling preparations into large-biomass fermentors. Another advantage of this system centres on the type of glycosylation that results, generally yielding protein ...
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Das Pichia pastoris-Expressionssystem

2012
Die methylotrophe Hefe Pichia pastoris (P. pastoris, Abb. 15–1) wurde in den letzten Jahren weltweit erfolgreich als System zur heterologen Genexpression etabliert und gehort mittlerweile neben Escherichia coli (Kap. 14), Saccharomyces cerevisiae, Hansenula polymorpha, dem Baculovirus-Expressionssystem (Kap. 14), Saugerzellsystemen (Kap.
Reinhart, C., Krettler, C.
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?????????? ?????????? ???????????????????? ?????????????? ???? ???????????????????????? ?? ???????????????????? ???????????? ?????????????????? Pichia guilliermondii

2021
?????????????????????? ?????????????? ?????????? ???????????????????? ???????????????? ???? ???????????????????????? ?? ?????????????????????? ???????????? ?????????????????? P. guilliermondii ?? ???????????????????? ???????????????????? ???????????????????? ??????????????????????. ????????????????, ?????? ???????????????????? ???????? ?????????????????
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Optimising Pichia pastoris Induction

2012
A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields ...
Zharain, Bawa, Richard A J, Darby
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Classical Genetics of Pichia pastoris

A significant advantage of P. pastoris as an experimental system and an eukaryotic expression platform is the ability to readily bring to bear both classical and molecular genetic approaches to a research problem and to develop technologies for manufacturing important proteins.
Ilya, Tolstorukov, Thomas G, Chappell, James M, Cregg   +2 more
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Pichia labacensis n.sp

Zeitschrift für allgemeine Mikrobiologie, 1984
Aleksa Cimhuman, Anna Kocrová-Kratochvilová   +1 more
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Transformation of Pichia pastoris by Electroporation

Transformation is one of the cornerstone techniques of molecular biotechnology, and it is an almost obligatory part of any study related to the investigation of gene functions, the production of recombinant proteins, or the alterations of the phenotypic features in Komagataella phaffii.
Kirill, Smirnov, Anton, Glieder
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Introduction to Pichia pastoris

1998
D R, Higgins, J M, Cregg
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Pexophagy in Pichia pastoris.

Methods in enzymology, 2009
The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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?????????????? ???????????????????????? ?????????????? ?? ???????????? rib1 ???? ?????????????????? ?????????????????????? ?? Pichia guilliermondii

2019
?? ???????????? ?????????????????????????????? ???????????? rib1-86 P. guilliermondii, ?? ?????????? ???????????????????????????????????????? ???????????????????????? ???????????????????????? ???????????????? rib81, ?????? ???? rib80. ?????????????????? ???????????? rib1-86 rib81 ???????????????????????????????? ?????????????? ???????????? ?????????????
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