Results 1 to 10 of about 21,587 (226)
Genotyping of recombinant Pichia pastoris strains [PDF]
Cellular and Molecular Biology Letters, 2006 A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector.
JOSÉ M Viader-Salvadó +1 more
exaly +6 more sources
Recombinant protein expression in Pichia pastoris
Molecular Biotechnology, 2000 The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein ...
James M Cregg, Cregg James M
exaly +4 more sources
Cloning and Extracellular Expression of Glargine in Pichia pastoris
Hayati Journal of Biosciences, 2023 Patients with diabetes mellitus increase significantly every year. The increasing number of people with diabetes mellitus results in increased insulin requirements.
Dudi Hardianto +6 more
doaj +3 more sources
Dynamic Feeding for Pichia pastoris
, 2022 The knowledge of certain strain-specific parameters of recombinant Pichia pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. These parameters are commonly determined either by time-consuming and labor-intensive
Spadiut, Oliver, Pekarsky, Alexander
core +3 more sources
Expression of lignocellulolytic enzymes in
Microbial Cell Factories, 2012 Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea +3 more
doaj +4 more sources
Isolation and bioenergetic characterization of mitochondria fromPichia pastoris
Yeast, 2006 7 páginas, 2 figuras, 1 tabla -- PAGS nros. 307-313Pichia pastoris is a methylotrophic yeast of high biotechnological interest. The bioenergetic properties of mitochondria from Pichia pastoris have not yet been determined. We report on a protocol for the
Eduardo Rial
exaly +2 more sources
Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris [PDF]
BioengineeringEnzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj +2 more sources
Ecotoxicology and Environmental Safety, 2022 Ammonia emissions have become an important environmental challenge for the livestock industry. Probiotics are often used as additives to reduce ammonia, and the ammonia reduction efficiency of common probiotics is approximately 20–40%.
Kunxian Feng +6 more
doaj +1 more source
Transcriptomics and Genomics in Pichia pastoris
, 2010 Auch wenn die methylotrophe Hefe Pichia pastoris einer der wichtigsten Expressionswirtegeworden ist, war keine Genom Sequenz dieses Organismus öffentlich zugänglich ? bis insJahr 2009, als die ersten Draft-Genome veröffentlicht wurden.
Kueberl, Andreas
core +3 more sources
High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins. [PDF]
PLoS ONE, 2015 Endo-β-N-acetylglucosaminidase H (Endo H, EC3.2.1.96) is a glycohydrolase that is widely used in the study of glycoproteins. The present study aimed to assess the effect of high-level endo-β-N-acetylglucosaminidase H expression in Pichia pastoris.
Fei Wang +6 more
doaj +1 more source