Results 21 to 30 of about 21,652 (231)
BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
doaj +1 more source
Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. [PDF]
PLoS ONE, 2013 The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene.
Hong Hu +9 more
doaj +1 more source
Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]
, 2010 Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried +9 more
core +1 more source
Glycosylation of thrombospondin type 1 repeats [PDF]
, 2008 In the course of mutagenesis of the forth thrombospondin type 1 repeat (TSR4) of the axonal guidance protein F-spondin, one mutant designated ΔDS2,3-TSR4 missing two out of three cystein bridges was found to lack the disaccharide Glcβ1,3-Fuc-O.
Krantz, Carsten
core +1 more source
Trends in Peptide and Protein Sciences, 2017 The use of the methylotrophic yeast, Pichia pastoris, as one of the most effective and versatile systems for the expression of heterologous proteins in biopharmaceutical manufacturing has become increasingly popular in recent years.
Atefeh Namipashaki +3 more
doaj +1 more source
Metabolic engineering of Pichia pastoris
Metabolic Engineering, 2018 Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin. We summarize here the impressive methodological developments of the last years to model and analyze the metabolism of P.
David A, Peña +4 more
openaire +2 more sources
Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris [PDF]
, 2007 The lipase genes of Yarrowia lipolytica, LIPY7 and LIPY8, fused with FLO-flocculation domain sequence from Saccharomyces cerevisiae at their N-termini, were expressed in Pichia pastoris KM71.
Song, Hui-Ting +5 more
core +1 more source
CHIMIA, 2005 During our work, flow cytometry was used for a study of the influence of cultivation conditions on a physiological state, mainly on the viability and vitality of the recombinant Pichia pastoris strain producing ?-galactosidase.
Petr Hyka +3 more
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Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
Synthetic and Systems Biotechnology, 2022 The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) has been extensively engineered for protein production, and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.
Rui Hou +6 more
doaj +1 more source
Increasing the production of insulin glargine in Pichia pastoris through medium modification [PDF]
BIO Web of ConferencesInsulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical ...
Hardianto Dudi +7 more
doaj +1 more source