Results 21 to 30 of about 37,837 (220)
Autolysis of Pichia pastoris induced by cold [PDF]
AMB Express, 2017 The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins.
Yaneth Bartolo-Aguilar +6 more
openaire +3 more sources
An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
openaire +3 more sources
Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. [PDF]
PLoS ONE, 2013 The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene.
Hong Hu +9 more
doaj +1 more source
Metabolic engineering of Pichia pastoris
Metabolic Engineering, 2018 Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin. We summarize here the impressive methodological developments of the last years to model and analyze the metabolism of P.
David A, Peña +4 more
openaire +2 more sources
Comparative genomics and transcriptomics of Pichia pastoris [PDF]
BMC Genomics, 2016 Pichia pastoris has emerged as an important alternative host for producing recombinant biopharmaceuticals, owing to its high cultivation density, low host cell protein burden, and the development of strains with humanized glycosylation. Despite its demonstrated utility, relatively little strain engineering has been performed to improve Pichia, due in ...
Love, Kerry R. +10 more
openaire +5 more sources
Trends in Peptide and Protein Sciences, 2017 The use of the methylotrophic yeast, Pichia pastoris, as one of the most effective and versatile systems for the expression of heterologous proteins in biopharmaceutical manufacturing has become increasingly popular in recent years.
Atefeh Namipashaki +3 more
doaj +1 more source
Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris [PDF]
, 2011 Includes bibliographical references (leaves 98-103).The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics.
Bhardwaj, Vinayak
core +1 more source
CHIMIA, 2005 During our work, flow cytometry was used for a study of the influence of cultivation conditions on a physiological state, mainly on the viability and vitality of the recombinant Pichia pastoris strain producing ?-galactosidase.
Petr Hyka +3 more
doaj +1 more source
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
Synthetic and Systems Biotechnology, 2022 The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) has been extensively engineered for protein production, and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.
Rui Hou +6 more
doaj +1 more source
Increasing the production of insulin glargine in Pichia pastoris through medium modification [PDF]
BIO Web of ConferencesInsulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical ...
Hardianto Dudi +7 more
doaj +1 more source