Ecophysiological approaches to enhance production of the anti-cancer drug taxol by paraconiothyrium variabile and epiccocum nigrum and lysozyme by pichia pastoris [PDF]
, 2013 This study has investigated the interaction of ecophysiological factors such as water activity (aw), temperature, pH and solute types on (a) the production of the anti-cancer drug taxol by the endophytic fungi Parachoniothyrium variabile and Epcoccum ...
Somjaipeng, Supunnika
core +7 more sources
Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris [PDF]
, 2013 Background: Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in ...
Harvey, Linda M. +4 more
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High-yield overproduction and purification of human aquaporins from Pichia pastoris
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair +7 more
doaj +1 more source
Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
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BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
doaj +1 more source
Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. [PDF]
PLoS ONE, 2013 The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene.
Hong Hu +9 more
doaj +1 more source
Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris [PDF]
, 2011 Includes bibliographical references (leaves 98-103).The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics.
Bhardwaj, Vinayak
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Trends in Peptide and Protein Sciences, 2017 The use of the methylotrophic yeast, Pichia pastoris, as one of the most effective and versatile systems for the expression of heterologous proteins in biopharmaceutical manufacturing has become increasingly popular in recent years.
Atefeh Namipashaki +3 more
doaj +1 more source
CHIMIA, 2005 During our work, flow cytometry was used for a study of the influence of cultivation conditions on a physiological state, mainly on the viability and vitality of the recombinant Pichia pastoris strain producing ?-galactosidase.
Petr Hyka +3 more
doaj +1 more source
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
Synthetic and Systems Biotechnology, 2022 The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) has been extensively engineered for protein production, and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.
Rui Hou +6 more
doaj +1 more source