Results 11 to 20 of about 21,652 (231)
Dynamic Feeding for Pichia pastoris [PDF]
, 2022 The knowledge of certain strain-specific parameters of recombinant Pichia pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. These parameters are commonly determined either by time-consuming and labor-intensive continuous cultivations or by several, consecutive fed-batch cultivations. Here, we describe a fast
Alexander, Pekarsky, Oliver, Spadiut
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Expression of lignocellulolytic enzymes in
Microbial Cell Factories, 2012 Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea +3 more
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Transcriptomics and Genomics in Pichia pastoris [PDF]
, 2010 Auch wenn die methylotrophe Hefe Pichia pastoris einer der wichtigsten Expressionswirtegeworden ist, war keine Genom Sequenz dieses Organismus öffentlich zugänglich ? bis insJahr 2009, als die ersten Draft-Genome veröffentlicht wurden. Zu dieser Zeit begannen auchdie in dieser Masterarbeit zusammengefassten Sequenzierarbeiten für den Wild-Typ ...
Kueberl, Andreas
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CLONING A FOREIGN GENE IN THE YEAST PICHIA PASTORIS [PDF]
, 2022 The S gene encoding the hepatitis B surface antigen was cloned into the yeast Pichia pastoris using the pPIC9 transfer vector. The recombinant plasmid pPIC9-S was treated with the restriction enzyme SacI and inserted into the yeast Pichia pastoris via ...
O.Ashirov T.Sa'dullayev A.Yarilkaganova A.Normatov S.Sasmakov Sh.Azimova
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Cloning and Expression of Yak Active Chymosin in [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production ...
Fan Luo +4 more
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An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
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High-yield overproduction and purification of human aquaporins from Pichia pastoris
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair +7 more
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Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
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Autolysis of Pichia pastoris induced by cold [PDF]
AMB Express, 2017 The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins.
Yaneth Bartolo-Aguilar +6 more
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Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris [PDF]
, 2013 Background: Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in ...
Harvey, Linda M. +11 more
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