Results 11 to 20 of about 37,837 (220)
Expression of lignocellulolytic enzymes in
Microbial Cell Factories, 2012 Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea +3 more
doaj +4 more sources
Recombinant protein expression in Pichia pastoris
Molecular Biotechnology, 2000 The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
J M, Cregg +3 more
openaire +3 more sources
Ecophysiological approaches to enhance production of the anti-cancer drug taxol by paraconiothyrium variabile and epiccocum nigrum and lysozyme by pichia pastoris [PDF]
, 2013 This study has investigated the interaction of ecophysiological factors such as water activity (aw), temperature, pH and solute types on (a) the production of the anti-cancer drug taxol by the endophytic fungi Parachoniothyrium variabile and Epcoccum ...
Somjaipeng, Supunnika
core +7 more sources
Towards better understanding of an industrial cell factory : investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris [PDF]
, 2013 Background: Novel analytical tools, which shorten the long and costly development cycles of biopharmaceuticals are essential. Metabolic flux analysis (MFA) shows great promise in improving our understanding of the metabolism of cell factories in ...
Harvey, Linda M. +4 more
core +4 more sources
Transcriptomics and Genomics in Pichia pastoris
, 2010 Auch wenn die methylotrophe Hefe Pichia pastoris einer der wichtigsten Expressionswirtegeworden ist, war keine Genom Sequenz dieses Organismus öffentlich zugänglich ? bis insJahr 2009, als die ersten Draft-Genome veröffentlicht wurden. Zu dieser Zeit begannen auchdie in dieser Masterarbeit zusammengefassten Sequenzierarbeiten für den Wild-Typ ...
Kueberl, Andreas
core +4 more sources
Sensors, 2023 This paper introduces a novel soft sensor modeling method based on BDA-IPSO-LSSVM designed to address the issue of model failure caused by varying fermentation data distributions resulting from different operating conditions during the fermentation of ...
Bo Wang, Jun Liu, Ameng Yu, Haibo Wang
doaj +1 more source
Cloning and Expression of Yak Active Chymosin in [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production ...
Fan Luo +4 more
doaj +1 more source
High-yield overproduction and purification of human aquaporins from Pichia pastoris
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair +7 more
doaj +1 more source
Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
core +1 more source
BMC Biotechnology, 2008 Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu +5 more
doaj +1 more source