Results 11 to 20 of about 21,587 (226)
Microbial Cell Factories, 2021 Background Codon optimization is a common method to improve protein expression levels in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to match the codon usage bias.
Yide Huang +6 more
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PERSPECTIVE DE PRODUCERE A HORMONULUI BIOSIMILAR DE CREȘTERE UMAN RECOMBINAT ÎN REPUBLICA MOLDOVA
Buletinul Academiei de Ştiinţe a Moldovei: Ştiinţe Medicale, 2022 În cadrul colaborării între USMF «N.Testemițanu», întreprinderea farmaceutică Balkan Pharmaceuticals și ICGEB a fost efectuat transferul tehnologic la etapa extrapolării de la faza de laborator la faza pilot a proceselor creării băncii celulelor de lucru
Liliana RUSNAC +4 more
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Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]
Brazilian Archives of Biology and Technology, 2020 Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos +12 more
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Alexandria Engineering Journal, 2022 The Pichia pastoris fermentation process is with highly nonlinear and time-varying and strong coupling characteristics, traditional control methods such as classic PID cannot satisfy the actual needs of the fermentation process control.
Bo Wang +4 more
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Microbial Cell Factories, 2020 Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro.
Jinbo Deng +12 more
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Sensors, 2023 This paper introduces a novel soft sensor modeling method based on BDA-IPSO-LSSVM designed to address the issue of model failure caused by varying fermentation data distributions resulting from different operating conditions during the fermentation of ...
Bo Wang, Jun Liu, Ameng Yu, Haibo Wang
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Cloning and Expression of Yak Active Chymosin in [PDF]
Asian-Australasian Journal of Animal Sciences, 2016 Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production ...
Fan Luo +4 more
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An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
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High-yield overproduction and purification of human aquaporins from Pichia pastoris
STAR Protocols, 2022 Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair +7 more
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Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]
, 2005 CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J. +11 more
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