Results 31 to 40 of about 21,652 (231)

Comparative genomics and transcriptomics of Pichia pastoris [PDF]

BMC Genomics, 2016
Pichia pastoris has emerged as an important alternative host for producing recombinant biopharmaceuticals, owing to its high cultivation density, low host cell protein burden, and the development of strains with humanized glycosylation. Despite its demonstrated utility, relatively little strain engineering has been performed to improve Pichia, due in ...
Love, Kerry R., Shah, Kartik A., Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel L., Priest, Margaret, Borowsky, Jonathan, Love, J. Christopher, Young, Sarah   +10 more
openaire   +5 more sources

Novel approaches for the creation of artificial metalloenzymes [PDF]

, 2013
Artificial metalloenzymes can be defined as the fusion of chemo- and enzymatic catalysts by insertion of non-specific achiral catalytic moieties into the chiral environment of a protein cavity, thereby broadening the scope of both fields.
Pereira Nogueira, Elisa Sofia
core   +1 more source

Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums

Microbial Cell Factories, 2023
Background Recently, researchers have focused on the search for alternatives to conventional antibiotics. Antimicrobial peptides are small bioactive peptides that regulate immune activation and have antibacterial activity with a reduced risk of bacterial
Chunming Dong, Lijun Xu, Weitao Lu, Mengru Li, Rui Zhang, Yanyan Sun, Jian Liu, Xinlei Chu   +7 more
doaj   +1 more source

Development of quantitative metabolomics for Pichia pastoris [PDF]

Metabolomics, 2011
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of extracellular
Carnicer,, M. (author), Canelas, A.B. (author), Ten Pierick, A. (author), Zeng, Z. (author), Van Dam, J. (author), Albiol, J. (author), Ferrer, P. (author), Heijnen, J.J. (author), Van Gulik, W. (author)   +8 more
openaire   +4 more sources

High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris [PDF]

, 2010
By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed ...
Speight, Robert, Winkler, Margit, Robert Speight, Nahalka, Jozef, Glieder, Anton, Anton Glieder, Margit Winkler, Sandra Abad, Abad, Sandra, Jozef Nahalka, Bergler, Gabriele, Bernd Nidetzky, Gabriele Bergler, Nidetzky, Bernd   +13 more
core   +1 more source

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Journal of Fungi
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications.
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder   +6 more
doaj   +1 more source

Preparation of Pichia pastoris Expression Plasmids [PDF]

, 2012
When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, C, Alkhalfioui, F, Byrne, B, Wagner, R   +3 more
openaire   +3 more sources

Overexpression of alcohol oxidase in Pichia pastoris [PDF]

FEBS Letters, 1991
The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein.
Hoop, M.J. de, Cregg, J., Keizer-Gunnink, I., Sjollema, Klaas, Veenhuis, M., Ab, G.   +5 more
openaire   +2 more sources

Constitutive expression of Camelus bactrianus prochymosin B in Pichia pastoris

Heliyon, 2021
Camel chymosin can be efficiently employed to produce cheese. Traditionally the rennet enzyme produced by the glands of the fourth stomach of ruminant animals (abomassum) is used in cheese making.
Zhiger Akishev, Assel Kiribayeva, Arman Mussakhmetov, Kairat Baltin, Yerlan Ramankulov, Bekbolat Khassenov   +5 more
doaj   +1 more source

Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes

VacciMonitor, 2021
La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez, Lourdes Zumalacárregui-de-Cárdenas, Zeila Santana-Vázquez   +2 more
doaj