Results 31 to 40 of about 37,837 (220)

Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]

, 2003
The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
core   +2 more sources

Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums

Microbial Cell Factories, 2023
Background Recently, researchers have focused on the search for alternatives to conventional antibiotics. Antimicrobial peptides are small bioactive peptides that regulate immune activation and have antibacterial activity with a reduced risk of bacterial
Chunming Dong, Lijun Xu, Weitao Lu, Mengru Li, Rui Zhang, Yanyan Sun, Jian Liu, Xinlei Chu   +7 more
doaj   +1 more source

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Journal of Fungi
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications.
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder   +6 more
doaj   +1 more source

Constitutive expression of Camelus bactrianus prochymosin B in Pichia pastoris

Heliyon, 2021
Camel chymosin can be efficiently employed to produce cheese. Traditionally the rennet enzyme produced by the glands of the fourth stomach of ruminant animals (abomassum) is used in cheese making.
Zhiger Akishev, Assel Kiribayeva, Arman Mussakhmetov, Kairat Baltin, Yerlan Ramankulov, Bekbolat Khassenov   +5 more
doaj   +1 more source

Development of quantitative metabolomics for Pichia pastoris [PDF]

Metabolomics, 2011
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of extracellular
Carnicer,, M. (author), Canelas, A.B. (author), Ten Pierick, A. (author), Zeng, Z. (author), Van Dam, J. (author), Albiol, J. (author), Ferrer, P. (author), Heijnen, J.J. (author), Van Gulik, W. (author)   +8 more
openaire   +4 more sources

Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes

VacciMonitor, 2021
La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez, Lourdes Zumalacárregui-de-Cárdenas, Zeila Santana-Vázquez   +2 more
doaj  

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Communications Biology, 2023
Polyehylene terephthalate (PET) biodegradation is greatly improved when recombinant IsPETase is expressed in Pichia pastoris and the enzyme’s partial removal of N-terminal glycosylation, retaining high thermostability.
Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai   +9 more
doaj   +1 more source

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Synthetic and Systems Biotechnology, 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj   +1 more source

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli [PDF]

PeerJ, 2020
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia
Yonggang Wang, Jiangqin Wang, Feifan Leng, Jianzhong Ma, Alnoor Bagadi   +4 more
doaj   +2 more sources

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source