Results 31 to 40 of about 26,443 (124)

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli [PDF]

PeerJ, 2020
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia
Yonggang Wang, Jiangqin Wang, Feifan Leng, Jianzhong Ma, Alnoor Bagadi   +4 more
doaj   +2 more sources

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Microbial Cell Factories
Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega   +5 more
doaj   +1 more source

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source

Enabling Bioprocess Upscaling Prediction Through Hybrid Modelling and Transfer Learning Under Small‐Data Scenarios

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Accurate prediction of bioprocess scale‐up is critical for accelerating the deployment of novel and sustainable biomanufacturing systems. However, this remains challenging as multi‐scale data is expensive to generate and mechanistic understanding is often incomplete, leading upscaling decisions to rely heavily on empirical expertise. This work
Harvey Al‐Ramadhan, Fernando Vega‐Ramon, Keju Jing, Dongda Zhang   +3 more
wiley   +1 more source

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris

Hayati Journal of Biosciences
Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti   +5 more
doaj   +1 more source

A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

MATEC Web of Conferences, 2015
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
doaj   +1 more source

Engineered immunostimulatory H2O2‐producing microreactors driven by yeast as localized living cancer therapeutics

BMEMat, EarlyView.
Engineered yeast microreactors that produce H2O2 within tumors enable selective cancer cell killing while releasing immunostimulatory components, effectively suppressing local tumor growth and activating systemic antitumor immunity, with further efficacy enhanced by anti‐PD‐1 therapy and transarterial embolization.
Wenqian He, Chunjie Wang, Minming Chen, Weihao Yang, Bin Yu, Yuhang Hu, Hengze Ding, Yifan Yan, Zhijuan Yang, Liangzhu Feng   +9 more
wiley   +1 more source

Quantifying Actin Persistence Length in S. pombe and S. cerevisiae: The Impact of Data Size, Filament Length and Fitting Strategy

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments exhibit isoform and species‐specific mechanical properties that are commonly quantified via their persistence length, yet the reliability of such measurements depends not only on the analysis method but also on dataset characteristics.
Peter Nietmann, Andrejus Suchenko, Andreas Janshoff, Mohan K. Balasubramanian   +3 more
wiley   +1 more source

Putting System Co‐Design at the Center of Chemo‐Biohybrid CO2 Valorization From Lab to Industrial Scale

EcoEnergy, EarlyView.
Chemo‐biohybrid systems promise much, but single‐discipline research still limits progress. This perspective draws on thermocatalytic, electrocatalytic, and photocatalytic hybrid systems to highlight the need for system‐level co‐design and outline future directions.
Wenwen Yu, Hanjun Sun, Long Liu
wiley   +1 more source