Results 31 to 40 of about 21,587 (226)

Comparative genomics and transcriptomics of Pichia pastoris [PDF]

BMC Genomics, 2016
Pichia pastoris has emerged as an important alternative host for producing recombinant biopharmaceuticals, owing to its high cultivation density, low host cell protein burden, and the development of strains with humanized glycosylation. Despite its demonstrated utility, relatively little strain engineering has been performed to improve Pichia, due in ...
Love, Kerry R., Shah, Kartik A., Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel L., Priest, Margaret, Borowsky, Jonathan, Love, J. Christopher, Young, Sarah   +10 more
openaire   +4 more sources

Increasing the production of insulin glargine in Pichia pastoris through medium modification [PDF]

BIO Web of Conferences
Insulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical ...
Hardianto Dudi, Safarrida Anna, Prayoga Gilang Surya, Ananda Zr Yufva Pramudia, Munirah, Martius Efrida, Royani Juwartina Ida, Hartuti Endah Dwi   +7 more
doaj   +1 more source

Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums

Microbial Cell Factories, 2023
Background Recently, researchers have focused on the search for alternatives to conventional antibiotics. Antimicrobial peptides are small bioactive peptides that regulate immune activation and have antibacterial activity with a reduced risk of bacterial
Chunming Dong, Lijun Xu, Weitao Lu, Mengru Li, Rui Zhang, Yanyan Sun, Jian Liu, Xinlei Chu   +7 more
doaj   +1 more source

Novel approaches for the creation of artificial metalloenzymes [PDF]

, 2013
Artificial metalloenzymes can be defined as the fusion of chemo- and enzymatic catalysts by insertion of non-specific achiral catalytic moieties into the chiral environment of a protein cavity, thereby broadening the scope of both fields.
Pereira Nogueira, Elisa Sofia
core   +1 more source

CLONING A FOREIGN GENE IN THE YEAST PICHIA PASTORIS

, 2022
The S gene encoding the hepatitis B surface antigen was cloned into the yeast Pichia pastoris using the pPIC9 transfer vector. The recombinant plasmid pPIC9-S was treated with the restriction enzyme SacI and inserted into the yeast Pichia pastoris via ...
O.Ashirov T.Sa'dullayev A.Yarilkaganova A.Normatov S.Sasmakov Sh.Azimova
core   +1 more source

Constitutive expression of Camelus bactrianus prochymosin B in Pichia pastoris

Heliyon, 2021
Camel chymosin can be efficiently employed to produce cheese. Traditionally the rennet enzyme produced by the glands of the fourth stomach of ruminant animals (abomassum) is used in cheese making.
Zhiger Akishev, Assel Kiribayeva, Arman Mussakhmetov, Kairat Baltin, Yerlan Ramankulov, Bekbolat Khassenov   +5 more
doaj   +1 more source

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Communications Biology, 2023
Polyehylene terephthalate (PET) biodegradation is greatly improved when recombinant IsPETase is expressed in Pichia pastoris and the enzyme’s partial removal of N-terminal glycosylation, retaining high thermostability.
Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai   +9 more
doaj   +1 more source

Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Synthetic and Systems Biotechnology, 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj   +1 more source

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli [PDF]

PeerJ, 2020
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia
Yonggang Wang, Jiangqin Wang, Feifan Leng, Jianzhong Ma, Alnoor Bagadi   +4 more
doaj   +2 more sources

Development of quantitative metabolomics for Pichia pastoris [PDF]

Metabolomics, 2011
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of extracellular
Carnicer,, M. (author), Canelas, A.B. (author), Ten Pierick, A. (author), Zeng, Z. (author), Van Dam, J. (author), Albiol, J. (author), Ferrer, P. (author), Heijnen, J.J. (author), Van Gulik, W. (author)   +8 more
openaire   +4 more sources