Asking the 5 W's for designing next‐generation bioprocessing
AIChE Journal, EarlyView.Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook +4 more
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Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]
, 2003 The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
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Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling
Biotechnology and Bioengineering, EarlyView.Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro +3 more
wiley +1 more source
Supplementary Materials of Annex I. High throughput stationary 13C-based Metabolic Flux Analysis of Pichia pastoris strains. PhD Thesis Albert Fina Romero [PDF]
, 2022 Altres ajuts: European Molecular Biology Organization (EMBO) - Short-Term Fellowship 8853Supplementary materials to the protocol describing High Throughput Stationary 13C-Metabolic Flux Analysis in Pichia pastoris.
Albiol i Sala, Joan +2 more
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Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture
Biotechnology and Bioengineering, EarlyView.ABSTRACT The high cost of cultivation media remains one of the barriers to the commercialization of cultivated meat. Current serum‐free media reduce reliance on fetal bovine serum but only achieve modest cost savings. Especially at larger scales (> 20 m3), pharmaceutical‐grade amino acids and recombinant proteins remain major cost drivers.
Jelle C. Hoek +3 more
wiley +1 more source
Genetic tools for metabolic engineering of Pichia pastoris
Engineering Microbiology, 2023 The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential in producing chemicals from inexpensive feedstocks, which requires efficient genetic engineering platforms.
Xiaoyan Wu +3 more
openaire +3 more sources
Вивчення механізмів деградації фруктозо-1,6-бісфосфатази у метилотрофних дріжджів Pichia pastoris
Фактори експериментальної еволюції організмів, 2018 Мета. Дослідити шляхи деградації фруктозо-1,6-бісфосфатази у метилотрофних дріжджів Pichia pastoris. Методи. Використовувалися методи визначення питомої активності фруктозо-1,6-бісфосфатази у дикого і мутантних штамах метилотрофних дріжджів P.
O. V. Dmytruk +2 more
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Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
Journal of Fungi, 2023 Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha +4 more
doaj +1 more source
Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development
Biotechnology and Bioengineering, EarlyView.This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma +6 more
wiley +1 more source
Expression of functional plant lectins in heterologous systems [PDF]
, 2000 The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation.
Raemaekers, Romaan J.M.
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