Results 51 to 60 of about 36,422 (221)

Pay32p of the Yeast Yarrowia lipolytica Is an Intraperoxisomal Component of the Matrix Protein Translocation Machinery [PDF]

, 1995
Pay mutants of the yeast Yarrowia lipolytica fail to assemble functional peroxisomes. One mutant strain, pay32-1, has abnormally small peroxisomes that are often found in clusters surrounded by membranous material.
Rachubinski, Richard A.,, Szilard, Rachel K.,, Titorenko, Vladimir I.,, Veenhuis, Marten,   +3 more
core   +3 more sources

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Microbial Cell Factories
Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega   +5 more
doaj   +1 more source

A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

MATEC Web of Conferences, 2015
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
doaj   +1 more source

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system : application on Pichia pastoris producing Rhizopus oryzae lipase [PDF]

, 2014
Background: In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (PAOX1), induced by methanol, present low reproducibility ...
Adelantado Vallvé, Núria, Barrigón de San Marcos, José Manuel, Ferrer, Pau, Hemmerich, Johannes, Hörmann, Astrid, Kensy, Frank, Ponte, Xavier, Valero Barranco, Francisco   +7 more
core   +4 more sources

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris

Hayati Journal of Biosciences
Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti   +5 more
doaj   +1 more source

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Journal of Fungi, 2023
Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha, Dan Liu, Juan Ren, Zhijun Liu, Xia Wu   +4 more
doaj   +1 more source

Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33 [PDF]

, 2015
BACKGROUND: The infections caused by antibiotic multidrug-resistant bacteria seriously threaten human health. To prevent and cure the infections caused by multidrug-resistant bacteria, new antimicrobial agents are required.
Da Teng, Jian Jiao, Jianhua Wang, Ruoyu Mao, Xintao Cao, Xiumin Wang, Yong Zhang   +6 more
core   +1 more source

Comparative genomics and transcriptomics of Pichia pastoris [PDF]

BMC Genomics, 2016
Pichia pastoris has emerged as an important alternative host for producing recombinant biopharmaceuticals, owing to its high cultivation density, low host cell protein burden, and the development of strains with humanized glycosylation. Despite its demonstrated utility, relatively little strain engineering has been performed to improve Pichia, due in ...
Love, Kerry R., Shah, Kartik A., Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel L., Priest, Margaret, Borowsky, Jonathan, Love, J. Christopher, Young, Sarah   +10 more
openaire   +3 more sources

Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris : functional characterisation of a novel promoter [PDF]

, 2017
As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates, it is a desirable production system. The function of a novel synthetic variant of the AOX1 promoter was characterised comprehensively using a strain ...
Hecht, Katrin, Kovar, Karin, Looser, Verena, Lüthy, Dominik, Melzoch, Karel, Straumann, Marcel   +5 more
core   +1 more source