Results 51 to 60 of about 26,443 (124)

Enhancing production of lipase MAS1 from marine Streptomyces sp. strain in Pichia pastoris by chaperones co-expression

Electronic Journal of Biotechnology, 2016
Background: A thermostable lipase MAS1 from marine Streptomyces sp. strain was considered as a potential biocatalyst for industrial application, but its production level was relatively low.
Dongming Lan, Man Qu, Bo Yang, Yonghua Wang   +3 more
doaj   +1 more source

A Rapid Lithium Ion‐Based Transformation Method for Kluyveromyces lactis

Yeast, EarlyView.
ABSTRACT Kluyveromyces lactis is a budding yeast host useful for recombinant protein production; however, its transformation often relies on electroporation due to the perceived low efficiency of chemical methods, thereby limiting throughput. In this study, we developed a robust, small‐scale chemical transformation protocol (using 15 μL of competent ...
Daisuke Tsugama, Ziyi Zuo
wiley   +1 more source

Functional and transcriptomic analyses in Neurospora crassa reveal a crucial role for the N‐glycoprotein deglycosylation process in fungal homeostasis

The FEBS Journal, EarlyView.
In Neurospora crassa, a cytosolic GH18 endo‐β‐n‐acetylglucosaminidase (ENGase) functions as an active deglycosylating enzyme. Deletion of this severely alters tolerance to multiple stress conditions and causes extensive transcriptomic reprogramming, including upregulation of a GH20 exo‐β‐n‐acetylhexosaminidase under ER stress, indicating that N. crassa
Anastasios Samaras, Tanim Jabid Hossain, Magnus Karlsson, Georgios Tzelepis   +3 more
wiley   +1 more source

Insights into tandem dioxygenases—copper radical oxidases in fungal Phytopathogens of the Colletotrichum acutatum species complex

The FEBS Journal, EarlyView.
In Colletotrichum acutatum species complex, conserved ring‐cleavage dioxygenases located adjacent to AA5_2 enzymes were investigated as potential redox partners. Biochemical analyses identified the dioxygenases as intradiol‐cleaving enzymes preferentially oxidizing methyl‐substituted catechols, while AA5_2 enzymes displayed weak alcohol oxidase ...
Radka Končitíková, David Ribeaucourt, Mireille Haon, David Navarro, Elodie Drula, Bastien Bissaro, Jean‐Guy Berrin, Mickael Lafond   +7 more
wiley   +1 more source

Production of enterocin P by a bioengineered Pichia pastoris to control of pathogen bacteria [PDF]

Journal of Epigenetics
Entrocin p has been characterized in class IIa bacteriocins and showed a broad spectrum of antimicrobial activity. In this study, the nucleotide sequence of enterocin P transformed and expressed in Pichia pastoris genomically, and antimicrobial activity ...
Abbas Tanhaeian, Reza Majidzadeh Heravi, bahareh pakbaten   +2 more
doaj   +1 more source

Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants

Plant, Cell &Environment, EarlyView.
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn, Fabian B. Haas, Benjamin G. Chavez, Bernd H. Dreyer, Erik V. Reiss, Anne Ziplys, Heiko Weichert, Saskia Hiltemann, José M. Ugalde, Andreas J. Meyer, John C. D'Auria, Stefan A. Rensing, Jos H. M. Schippers   +12 more
wiley   +1 more source

Tailored Covalent Organic Frameworks Enable the Encapsulation of Living Cells to Produce Rare Ginsenoside

Advanced Science, Volume 13, Issue 61, 3 November 2026.
This work reports the in situ fabrication of Escherichia coli@covalent organic frameworks (E. coli@COFs) in phosphate‐buffered saline. The COFs form an exoskeleton on E. coli, protecting intracellular β‐glucosidase (Ssbgl) while enhancing mass transfer.
Fengmei Liu, Shan Zhang, Shanrong Wei, Ziye Xing, Hongming He, Xiao Liang, Quanshun Li   +6 more
wiley   +1 more source

Pectinase‐Based Bioprocesses for Circular Bioeconomy and Sustainable Industrial Development

Biotechnology and Bioengineering, Volume 123, Issue 11, Page 2906-2936, November 2026.
This picture demonstrates a unified strategy for sustainable pectinase production, focusing on microbial bioproduction, innovative strategies in enzyme engineering (Protein engineering, rational enzyme design, directed evolution and heterologous expression) and various applications in industry, including food, textiles, pulp and paper, agriculture and ...
Nisha Sharma, Dimple Tanwar, Usha Grewal, Murugesan Mohana Keerthi, Yogesh K. Ahlawat, Nitin Pandey, Chirag G. Makvana   +6 more
wiley   +1 more source

Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris

Microbiology Indonesia, 2017
Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57
doaj   +1 more source

A Protocol for Lab‐Scale Production of 13C‐Labeled Yeast Extract as Internal Standard for Metabolomics and Quantification of Intracellular Metabolites

Current Protocols, Volume 6, Issue 11, November 2026.
Abstract Metabolomics enables the profiling of small‐molecule metabolites, thereby capturing the biochemical state of a living organism at a given moment and making it possible to monitor the organism's cellular responses to stimuli. This technique has become a powerful tool in pharmaceutical research, the food industry, and microbial research ...
Miriam Cammaert, Rosalie Wouters, Jitske van Ede, Erik de Hulster, Christiaan Mooiman, Patricia van Dam, Martin Pabst, Walter van Gulik, Pascale Daran‐Lapujade   +8 more
wiley   +1 more source