Results 51 to 60 of about 21,587 (226)

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Journal of Fungi, 2023
Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha, Dan Liu, Juan Ren, Zhijun Liu, Xia Wu   +4 more
doaj   +1 more source

Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris [PDF]

, 2003
The cDNA encoding the proenzyme of cathepsin C from the parasitic helminth Schistosoma mansoni was amplified from a cDNA library using 5’ and 3’ specific primers containing sites for SnaBI and Avrll digestion.
Carty, Charlene
core  

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
doaj   +1 more source

Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

BMC Biotechnology, 2021
Background Nattokinase is a fibrinolytic enzyme that has huge market value as a nutritional supplement for health promotion. In order to increase nattokinase yields, fermentation conditions, strains, cultivation media, and feeding strategies have been ...
Yan Guangbo, Shu Min, Shen Wei, Ma Lixin, Zhai Chao, Wang Yaping, Huang Zunxi   +6 more
doaj   +1 more source

Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling

Biotechnology and Bioengineering, EarlyView.
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro, José Luis Checa‐Barrera, Juan A. Moreno‐Cid, Eoin Casey   +3 more
wiley   +1 more source

Genetic tools for metabolic engineering of Pichia pastoris

Engineering Microbiology, 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential in producing chemicals from inexpensive feedstocks, which requires efficient genetic engineering platforms.
Xiaoyan Wu, Peng Cai, Lun Yao, Yongjin J Zhou   +3 more
openaire   +3 more sources

Enhancing production of lipase MAS1 from marine Streptomyces sp. strain in Pichia pastoris by chaperones co-expression

Electronic Journal of Biotechnology, 2016
Background: A thermostable lipase MAS1 from marine Streptomyces sp. strain was considered as a potential biocatalyst for industrial application, but its production level was relatively low.
Dongming Lan, Man Qu, Bo Yang, Yonghua Wang   +3 more
doaj   +1 more source

Expression of functional plant lectins in heterologous systems [PDF]

, 2000
The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation.
Raemaekers, Romaan J.M.
core  

Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris

, 2007
The lipase genes of Yarrowia lipolytica, LIPY7 and LIPY8, fused with FLO-flocculation domain sequence from Saccharomyces cerevisiae at their N-termini, were expressed in Pichia pastoris KM71.
Song, Hui-Ting, Wu, Zhen-Bin, Jiang, Zheng-Bing, Ma, Li-Xin, Jiang, ZB, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China, Gupta, Nishith   +5 more
core   +1 more source