Results 71 to 80 of about 26,443 (124)

Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris

open access: yes, 2007
The lipase genes of Yarrowia lipolytica, LIPY7 and LIPY8, fused with FLO-flocculation domain sequence from Saccharomyces cerevisiae at their N-termini, were expressed in Pichia pastoris KM71.
Song, Hui-Ting   +10 more
core   +1 more source

Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris

open access: yesCurrent Research in Biotechnology
Pichia pastoris is one of the most commonly used hosts for producing heterologous proteins, whereas production levels vary depending on the protein of interest and are also regulated by regulatory factors.
Shengyan Wang   +6 more
doaj   +1 more source

Expression of lignocellulolytic enzymes in Pichia pastoris

open access: yesMicrobial Cell Factories, 2012
Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea   +3 more
doaj   +1 more source

Metabolic engineering of Pichia pastoris for sustainable production of 1,8-cineole from methanol

open access: yesFrontiers in Microbiology
Methanol is an ideal feedstock for biomanufacturing and can be produced in large quantities from carbon dioxide via photocatalysis or electrolytic reduction, contributing to global carbon neutrality.
Cui Zhao   +7 more
doaj   +1 more source

Overview of insulin production and purification process from Pichia pastoris fermentation culture.

open access: yes, 2016
Overview of insulin production and purification process from Pichia pastoris fermentation culture.
Sergio G. Tisminetzky (3373163)   +6 more
core   +1 more source

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model

open access: yesParasite Epidemiology and Control, 2019
Introduction: Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication. Methods: An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2
Rajkumar Paul   +5 more
doaj   +1 more source

Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris

open access: yes, 1999
Optimization of the production of a honeybee odorant-binding protein by [i]Pichia pastoris ...
Danty-Berger, Emmanuelle   +6 more
core   +1 more source

Application of flocculation technology for solid-liquid separation of Pichia pastoris fermentation broth

open access: yesSiliao yanjiu
The experiment aims to use flocculation technology for solid-liquid separation of the fermentation broth of Pichia pastoris. With the flocculated cell rate and enzyme activity recovery rate as the measurement indicators, single-factor experiments and ...
LUO Tong-yang   +6 more
doaj  

Influence of Co-feeding Methanol-sorbitol Ratio on Production of Human Insulin Precursor Expressed by Mut+ Pichia pastoris

open access: yesHayati Journal of Biosciences
An increasing number of diabetic patients and the demand for insulin encourage the development of recombinant insulin production on a large scale. Human insulin precursor (HIP) expressed by Mut+ Pichia pastoris using methanol as an inducer was developed.
Dian Japany Puspitasari   +4 more
doaj   +1 more source

Production of recombinant human erythropoietin from Pichia pastoris and its structural analysis

open access: yes, 2007
Aims: To design and investigate a recombinant expression system producing a therapeutically important glycoprotein, human erythropoietin (rHuEPO), by Pichia ...
Halloran, S. M.   +6 more
core   +1 more source

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