Results 61 to 70 of about 26,443 (124)
Structure‐guided substrate‐pocket engineering and electron‐transfer optimization are integrated to enhance cellobiose dehydrogenase for lactose oxidation. The N313D mutation strengthens substrate‐binding interactions, and A165C and W316H improve electron transfer.
Xinyu Cui +6 more
wiley +1 more source
Optical Detection of Cellular Signals at Material Interfaces
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren +5 more
wiley +1 more source
A Portable Wireless Electrochemical Sensor for Ethanol Detection in Human Saliva
A portable wireless sensor integrates an enzyme‐modified gold electrode with a potentiostat to detect ethanol in saliva. Ethanol‐dependent electrochemical responses are demonstrated in artificial saliva and human saliva samples. The system provides a non‐invasive approach to monitoring salivary alcohol and may complement established breath‐based ...
Tae Mu Lee +3 more
wiley +1 more source
Recombinant gelatin and collagen from methylotrophic yeasts [PDF]
Based on its structural role and compatibility within the human body, collagen is a commonly used biomaterial in medical applications, such as cosmetic surgery, wound treatment and tissue engineering.
Bruin, E.C., de
core
K+ Channels in Membrane Potential Dynamics and Migration of Neutrophil Granulocytes
Neutrophil membrane potential dynamics regulate directed migration. Kir2.1 regulates neutrophil membrane potential and indirectly LTB4 secretion. Together with KCa3.1 and KV1.3, it is required for efficient chemotaxis. KCa3.1 and KV1.3 influence neutrophil migration without altering membrane potential, potentially through reduced LTB4 release (KCa3.1 ...
Tahir Ulu +7 more
wiley +1 more source
High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris
By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed ...
Speight, Robert +13 more
core +1 more source
Altres ajuts: European Molecular Biology Organization (EMBO) - Short-Term Fellowship 8853Supplementary materials to the protocol describing High Throughput Stationary 13C-Metabolic Flux Analysis in Pichia pastoris.
Albiol i Sala, Joan +2 more
core +1 more source
Pediocin (Ped) is a 4.6kDa bacteriocin effective against Listeria monocytogenes and several Gram-positive bacteria. To produce purified Ped with high yield and apply it to food preservation, this study expresses and purifies recombinant Ped (rPed) from ...
Nguyễn Phạm Anh Thư +2 more
doaj +1 more source
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA.
Nor Soleha Mohd Dali +6 more
doaj +1 more source
This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P.
Kerstin Lange +3 more
doaj +1 more source

