Results 61 to 70 of about 36,422 (221)
AMB Express, 2020 In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao +6 more
doaj +1 more source
BMC Biotechnology, 2021 Background Nattokinase is a fibrinolytic enzyme that has huge market value as a nutritional supplement for health promotion. In order to increase nattokinase yields, fermentation conditions, strains, cultivation media, and feeding strategies have been ...
Yan Guangbo +6 more
doaj +1 more source
Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris [PDF]
, 2014 Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of ...
Albiol i Sala, Joan +5 more
core +2 more sources
Transfer Learning Approaches in Bioprocess Engineering: Opportunities and Challenges
Biotechnology and Bioengineering, EarlyView.ABSTRACT Transfer learning (TL) has recently emerged as a promising approach to overcoming one of the key limitations of bioprocess engineering: data scarcity. By leveraging knowledge from one bioprocess to another, TL allows existing models and data sets to be reused efficiently, accelerating process development, improving prediction accuracy, and ...
Daniel Barón Díaz +3 more
wiley +1 more source
Electronic Journal of Biotechnology, 2016 Background: A thermostable lipase MAS1 from marine Streptomyces sp. strain was considered as a potential biocatalyst for industrial application, but its production level was relatively low.
Dongming Lan +3 more
doaj +1 more source
Development Of High Cell Density Recombinant Pichia Pastoris Culture For Human Growth Hormone Production [PDF]
, 2008 Methylotrophic yeast, Pichia pastoris has been used as an excellent host to produce more than 400 recombinant heterologous proteins of academic and industrial interests.
Lee, Yoeu Chek
core
A comparative study of peroxisomal structures in Hansenula polymorpha pex mutants [PDF]
, 2007 In a recent study, we performed a systematic genome analysis for the conservation of genes involved in peroxisome biogenesis (PEX genes) in various fungi.
Klei, Ida J. van der, +3 more
core +2 more sources
Synthetic Core Promoters for Pichia pastoris
ACS Synthetic Biology, 2013 Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostly been focused on altering upstream regulatory sequences such as transcription factor binding sites.
Vogl, Thomas +4 more
openaire +2 more sources
An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
openaire +3 more sources
Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics
Biotechnology and Bioengineering, EarlyView.ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu +4 more
wiley +1 more source