Results 41 to 50 of about 37,837 (220)

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Microbial Cell Factories
Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega   +5 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Preparation of Pichia pastoris Expression Plasmids [PDF]

, 2012
When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, Christel, Alkhalfioui, Fatima, Byrne, Bernadette, Wagner, Renaud   +3 more
openaire   +3 more sources

Glycosylation of thrombospondin type 1 repeats [PDF]

, 2008
In the course of mutagenesis of the forth thrombospondin type 1 repeat (TSR4) of the axonal guidance protein F-spondin, one mutant designated ΔDS2,3-TSR4 missing two out of three cystein bridges was found to lack the disaccharide Glcβ1,3-Fuc-O.
Krantz, Carsten
core   +1 more source

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris

Hayati Journal of Biosciences
Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti   +5 more
doaj   +1 more source

A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

MATEC Web of Conferences, 2015
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
doaj   +1 more source

Optical Detection of Cellular Signals at Material Interfaces

Advanced Healthcare Materials, EarlyView.
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren, Jitong Ren, Zixin Zhang, Koney Eungkon Kim, Francis L. Hong, Yuecheng Peter Zhou   +5 more
wiley   +1 more source

Dynamics in bioprocess development forPichia pastoris [PDF]

Bioengineered, 2014
Pichia pastoris is a widely used host organism for the recombinant production of proteins. It is attracting increasing interest for the production of biopharmaceuticals, due to its capability of performing posttranslational modifications. Traditionally, production processes with P.
Spadiut, Oliver, Herwig, Christoph
openaire   +2 more sources

Tailored Covalent Organic Frameworks Enable the Encapsulation of Living Cells to Produce Rare Ginsenoside

Advanced Science, EarlyView.
This work reports the in situ fabrication of Escherichia coli@covalent organic frameworks (E. coli@COFs) in phosphate‐buffered saline. The COFs form an exoskeleton on E. coli, protecting intracellular β‐glucosidase (Ssbgl) while enhancing mass transfer.
Fengmei Liu, Shan Zhang, Shanrong Wei, Ziye Xing, Hongming He, Xiao Liang, Quanshun Li   +6 more
wiley   +1 more source