Results 41 to 50 of about 26,443 (124)

Вивчення механізмів деградації фруктозо-1,6-бісфосфатази у метилотрофних дріжджів Pichia pastoris

Фактори експериментальної еволюції організмів, 2018
Мета. Дослідити шляхи деградації фруктозо-1,6-бісфосфатази у метилотрофних дріжджів Pichia pastoris. Методи. Використовувалися методи визначення питомої активності фруктозо-1,6-бісфосфатази у дикого і мутантних штамах метилотрофних дріжджів P.
O. V. Dmytruk, N. V. Bulbotka, A. A. Sibirny   +2 more
doaj   +1 more source

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Journal of Fungi, 2023
Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha, Dan Liu, Juan Ren, Zhijun Liu, Xia Wu   +4 more
doaj   +1 more source

Precision Fermentation for Next Generation Food Ingredients: Technological Foundations, Food Applications, Sustainability, and Regulatory Challenges

Food Bioengineering, EarlyView.
Precision fermentation uses engineered microorganisms to produce targeted food ingredients, including proteins, enzymes, lipids, flavors, vitamins, and bioactives. This review examines its technological foundations, food applications, safety, sustainability, economics, regulation, and consumer acceptance, emphasizing that performance depends on host ...
Nora Ali Hassan, Rawaa H. Tlay, Tarek Gamal Abedelmaksoud   +2 more
wiley   +1 more source

Open access to sequence: Browsing the Pichia pastoris genome

Microbial Cell Factories, 2009
The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have ...
Graf Alexandra, Lin Yao-Cheng, Rouzé Pierre, Callewaert Nico, Mattanovich Diethard, Redl Andreas, Tiels Petra, Gasser Brigitte, De Schutter Kristof   +8 more
doaj   +1 more source

Interactions between lactobacillus and yeast: A recent update

Food Biomacromolecules, EarlyView.
Abstract Lactobacillus and yeast are microorganisms widely distributed in nature. Their interactions significantly impact fermentation processes, human health, and medical care. However, there is a lack of a systematic summary of the characteristics of these interactions, and the mechanisms underlying these interactions remain unclear.
Yang Chen, Mengyuan Liu, Mengchen Li, Zhiyun Yue, Yang Mu, Chao Wang, Lusha Xia, Junjun Gao, Mingye Peng, Ran Wang, Mengzhou Zhou   +10 more
wiley   +1 more source

Expression of functional plant lectins in heterologous systems [PDF]

, 2000
The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin; GNA) was produced in Escherichia coli and purified as a functional protein after denturation/renaturation.
Raemaekers, Romaan J.M.
core  

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
doaj   +1 more source

Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

BMC Biotechnology, 2021
Background Nattokinase is a fibrinolytic enzyme that has huge market value as a nutritional supplement for health promotion. In order to increase nattokinase yields, fermentation conditions, strains, cultivation media, and feeding strategies have been ...
Yan Guangbo, Shu Min, Shen Wei, Ma Lixin, Zhai Chao, Wang Yaping, Huang Zunxi   +6 more
doaj   +1 more source

Analysis of Carbohydrates and Glycoconjugates by Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2023–2024

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The use of MALDI mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this comprehensive review is the twelfth update of the original article published in 1999 and brings coverage of the literature to the end of 2024.
David J. Harvey
wiley   +1 more source

Glycosylation of thrombospondin type 1 repeats [PDF]

, 2008
In the course of mutagenesis of the forth thrombospondin type 1 repeat (TSR4) of the axonal guidance protein F-spondin, one mutant designated ΔDS2,3-TSR4 missing two out of three cystein bridges was found to lack the disaccharide Glcβ1,3-Fuc-O.
Krantz, Carsten
core   +1 more source