Results 41 to 50 of about 21,587 (226)

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Journal of Fungi
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications.
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder   +6 more
doaj   +1 more source

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source

Preparation of Pichia pastoris Expression Plasmids [PDF]

, 2012
When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, C, Alkhalfioui, F, Byrne, B, Wagner, R   +3 more
openaire   +3 more sources

Overexpression of alcohol oxidase in Pichia pastoris [PDF]

FEBS Letters, 1991
The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein.
Hoop, M.J. de, Cregg, J., Keizer-Gunnink, I., Sjollema, Klaas, Veenhuis, M., Ab, G.   +5 more
openaire   +2 more sources

Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes

VacciMonitor, 2021
La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez, Lourdes Zumalacárregui-de-Cárdenas, Zeila Santana-Vázquez   +2 more
doaj  

Dynamics in bioprocess development forPichia pastoris [PDF]

Bioengineered, 2014
Pichia pastoris is a widely used host organism for the recombinant production of proteins. It is attracting increasing interest for the production of biopharmaceuticals, due to its capability of performing posttranslational modifications. Traditionally, production processes with P.
Spadiut, Oliver, Herwig, Christoph
openaire   +2 more sources

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Microbial Cell Factories
Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega   +5 more
doaj   +1 more source

A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

MATEC Web of Conferences, 2015
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
doaj   +1 more source

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris

Hayati Journal of Biosciences
Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti   +5 more
doaj   +1 more source