Results 81 to 90 of about 21,587 (226)

Novel Biopesticides Based on Recombinant Avidin for Protection of Crops Against Insect Pests [PDF]

, 2012
Farmers are required to increase their food productivity to meet the demand from a continually enlarging population. A major constraint on meeting this requirement is the damage created by insect pests.
HINCHLIFFE, GARETH
core  

Transfer Learning Approaches in Bioprocess Engineering: Opportunities and Challenges

Biotechnology and Bioengineering, Volume 123, Issue 6, Page 1417-1431, June 2026.
ABSTRACT Transfer learning (TL) has recently emerged as a promising approach to overcoming one of the key limitations of bioprocess engineering: data scarcity. By leveraging knowledge from one bioprocess to another, TL allows existing models and data sets to be reused efficiently, accelerating process development, improving prediction accuracy, and ...
Daniel Barón Díaz, Anna‐Lena Drommershausen, Alexander Grünberger, Dirk Holtmann   +3 more
wiley   +1 more source

Pichia pastoris as a Host System for Transformations [PDF]

Molecular and Cellular Biology, 1985
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors which contained either the P.
J M, Cregg, K J, Barringer, A Y, Hessler, K R, Madden   +3 more
openaire   +2 more sources

Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris

, 2011
Includes bibliographical references (leaves 98-103).The increasing prevalence of diabetes worldwide has greatly increased the demand for insulin, a key type of treatment for many diabetics.
Bhardwaj, Vinayak
core  

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, Volume 123, Issue 6, Page 1708-1720, June 2026.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

Metabolism of Hydrogen Peroxide byPichia pastoris [PDF]

Agricultural and Biological Chemistry, 1990
Hydrogen peroxide in methylotrophic yeasts can be metabolized in at least two distinct ways. Addition of exogenous hydrogen peroxide removes the dependance of catalase on endogenously-produced hydrogen peroxide resulting enhanced rates of alcohol oxidation.
DUFF, Sheldon J. B., MURRAY, William D.
openaire   +2 more sources

Molecular Cloning and Production of Recombinant Phytase from Bacillus subtilis ASUIA243 in Pichia pastoris

International Islamic University Malaysia Engineering Journal, 2011
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA.
Nor Soleha Mohd Dali, Tamrin Nuge, Mohd Hafidz Mahamad Maifiah, Faridah Yusof, Anis Shobirin Meor Hussin, Abd-Elaziem Farouk, and Hamzah Mohd. Salleh   +6 more
doaj   +1 more source

Structural Features and Reaction Profile of an Evolved Unspecific Peroxygenase From Candolleomyces Aberdarensis

Advanced Synthesis &Catalysis, Volume 368, Issue 9, 5 May 2026.
Fungal unspecific peroxygenases (UPOs) are promiscuous heme‐containing enzymes that selectively oxyfuntionalize C‐H bonds. Structural and enzymatic reaction studies of an evolved UPO mutant from Candolleomyces (Psathyrella) aberdarensis (PabUPO‐I) reveal how the heme channel architecture dictates the regioselectivity towards alkanes, fatty acids and ...
Andrea Menés‐Rubio, Angela Fernandez‐Garcia, Miquel A. Galmes‐Ordinas, Patricia Gomez de Santos, Israel Sanchez‐Moreno, Julia Sanz‐Aparicio, Miguel Alcalde   +6 more
wiley   +1 more source

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Microbial Cell Factories, 2008
AbstractBackgroundHigh cell density cultures ofPichia pastorisgrown on methanol tend to develop yellow colored supernatants, attributed to the release of free flavins. The potential ofP. pastorisfor flavin overproduction is therefore given, but not pronounced when the yeast is grown on glucose.
Mattanovich Diethard, Marx Hans, Sauer Michael   +2 more
openaire   +3 more sources

Expression, purification and application of recombinant Pediocin from Pichia pastoris X33::ped in shrimp preservation trial

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
Pediocin (Ped) is a 4.6kDa bacteriocin effective against Listeria monocytogenes and several Gram-positive bacteria. To produce purified Ped with high yield and apply it to food preservation, this study expresses and purifies recombinant Ped (rPed) from ...
Nguyễn Phạm Anh Thư, Đặng Thị Phương Thảo, Nguyễn Hiếu Nghĩa   +2 more
doaj   +1 more source