Results 81 to 90 of about 26,443 (124)

Growth curves of Pichia Pastoris cells.

, 2014
(A) Growth of Pichia pastoris cells in a hydrogenated medium at 30°C (red circle) and 18°C (green circle). (B) Growth of P. pastoris cells in a deuterated medium at 30°C (blue square) and 18°C (cyan square).
Giovanna Fragneto (586510), Alexis de Ghellinck (586504), Hubert Schaller (281018), Valérie Laux (586505), Hanna Wacklin (586508), Michael Haertlein (586506), Eric Maréchal (7818), Juliette Jouhet (586509), Michele Sferrazza (586507)   +8 more
core   +1 more source

Producción de una fitasa recombinante en Pichia pastoris [PDF]

, 2007
En el presente trabajo se construyeron cepas recombinantes de Pichia pastoris capaces de producir y secretar una fitasa de origen bacteriano, factible de ser empleada como aditivo en alimentos para animales.
Rodríguez Blanco, Lili, VIADER SALVADÓ, JOSÉ MA., Guerrero Olazarán, Martha, Viader Salvadó, José María   +3 more
core   +1 more source

Measuring intracellular calcium concenrations in Pichia pastoris

, 2013
Pichia pastoris, a methylotrophic yeast, is used for heterologous protein expression. Calcium is an important second messenger in signal transduction. Little research has been performed to analyze calcium homeostasis in P. pastoris.
Kim, Joseph, Bindra, Kavneet, Diaz, Stephanie, Dimont, Gregory   +3 more
core  

Expression of Fluorescently Tagged Proteins in Pichia pastoris

, 2014
The aim of this study was to recombinantly express Pyriform Spidroin 1 (PySp1) and Protein Kinase C (PKC1) in the yeast Pichia pastoris. PySp1 is a spider silk protein that we believe can be expressed and secreted in large amounts by Pichia pastoris for ...
Dong, Dale, Ota, Kasey, Chahal, Sabreen
core  

List of Pichia pastoris Strains Used in This Study.

List of Pichia pastoris Strains Used in This Study.
Katsuhiko Kamei (491451), Yoshitsugu Miyazaki (477146), Shogo Takatsuka (5864570), Emiko Sakaida (7344737), Shinobu Saijo (23401), Yutaro Hino (17729692), Akira Watanabe (134585), Sho Yamasaki (522606), Yasunori Muraosa (5146406)   +8 more
core   +1 more source

Pichia pastoris - HSA Core Production System Laboratory Manual

The Northeast Biomanufacturing Center and Collaborative (NBC2) has created four laboratory manuals provided as downloadable pdfs. The Pichia pastoris - HSA Core Production System laboratory manual is 108 pages; it includes standard operating procedures ...

core   +1 more source

Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris

Genetics and Molecular Biology, 2011
The Pichia pastoris expression system was used to produce recombinant human erythropoietin, a protein synthesized by the adult kidney and responsible for the regulation of red blood cell production.
Ser Huy Teh, Mun Yik Fong, Zulqarnain Mohamed   +2 more
doaj  

Use of a dual biosensor for identification of novel secretion signal peptides and efficient screening of precision fermentation production strains.

Future Foods
Methylotrophic yeast Pichia pastoris (Komagaetella phaffii) has emerged as a versatile and robust host for recombinant protein production, owing to its capacity for high-level protein expression, scalability, and post-translational protein modifications.
Parastoo Karimian, Ema Johnston, Annette Kasprzak, Jian-Wei Liu, Sally Stockwell, Thomas Vanhercke, CarolJ. Hartley   +6 more
doaj   +1 more source

Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes [PDF]

, 2005
Thesis (MSc)--Stellenbosch University, 2005.ENGLISH ABSTRACT: This study describes: I. The cloning of the CVP 19 gene and construction of the intracellular expression vector pPIC3.5K-CYP19. II.
Wepener, Ilse
core  

Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris

Bioengineering
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj   +1 more source