Results 81 to 90 of about 36,422 (221)

Enhanced heterologous protein production in Pichia pastoris under increased air pressure [PDF]

, 2014
Pichia pastoris is a widely used host for the production of heterologous proteins. In this case, high cell densities are needed and oxygen is a major limiting factor. The increased air pressure could be used to improve the oxygen solubility in the medium
Baumann, Belo, Belo, Belo, Bradford, Cereghino, Cereghino, Cereghino, Charoenrat, Cregg, Cunha, Goldfeld, Idiris, Jahic, Jin, Jones, Khatri, Knoll, Kobayashi, Laemmli, Lee, Liang, Lopes, Lopes, Lopes, Lopes, Lopes, Macauley-Patrick, Oliveira, Oliveira, Oliveira, Pinheiro, Pinheiro, Pinheiro, Potvin, Sinha, Surribas, Viader-Salvadó, Woo, Yamashita, Çelik   +40 more
core   +1 more source

Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants

Plant, Cell &Environment, EarlyView.
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn, Fabian B. Haas, Benjamin G. Chavez, Bernd H. Dreyer, Erik V. Reiss, Anne Ziplys, Heiko Weichert, Saskia Hiltemann, José M. Ugalde, Andreas J. Meyer, John C. D'Auria, Stefan A. Rensing, Jos H. M. Schippers   +12 more
wiley   +1 more source

Liquid PTVA: A faster and cheaper alternative for generating multi-copy clones in Pichia pastoris [PDF]

, 2016
Additional file 1: Figure S1.
Aw, R, Polizzi, KM
core   +5 more sources

PpAtg9 Trafficking During Micropexophagy in Pichia pastoris [PDF]

Autophagy, 2006
PpAtg9 is essential for the selective degradation of peroxisomes (e.g., pexophagy) in Pichia pastoris. This integral membrane protein is synthesized in the endoplasmic reticulum (ER) and transported to a unique peripheral compartment (Atg9-PC). A putative ER exit motif has been identified and when deleted results in the accumulation of PpAtg9 within ...
Laura A, Schroder, William A, Dunn
openaire   +2 more sources

Structural Features and Reaction Profile of an Evolved Unspecific Peroxygenase From Candolleomyces Aberdarensis

Advanced Synthesis &Catalysis, Volume 368, Issue 9, 5 May 2026.
Fungal unspecific peroxygenases (UPOs) are promiscuous heme‐containing enzymes that selectively oxyfuntionalize C‐H bonds. Structural and enzymatic reaction studies of an evolved UPO mutant from Candolleomyces (Psathyrella) aberdarensis (PabUPO‐I) reveal how the heme channel architecture dictates the regioselectivity towards alkanes, fatty acids and ...
Andrea Menés‐Rubio, Angela Fernandez‐Garcia, Miquel A. Galmes‐Ordinas, Patricia Gomez de Santos, Israel Sanchez‐Moreno, Julia Sanz‐Aparicio, Miguel Alcalde   +6 more
wiley   +1 more source

Novel homologous lactate transporter improves l‑lactic acid production from glycerol in recombinant strains of Pichia pastoris [PDF]

, 2016
Background: Crude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude ...
Carvalho, Lucas Silva, Castro, Virgilio H. de, Goldman, Gustavo Henrique, Lima, Pollyne Borborema Almeida de, Magalhães, Beatriz Simas, Melo, Nadiele Tamires Moreira, Menino, Gisele Soares, Mulder, Kelly Cristina Leite, Mulinari, Eduardo, Parachi, Nádia Skorupa, Reis, Thaila Fernanda dos   +10 more
core   +1 more source

Engineering a Synthetic Formatotrophic Yeast With Enhanced Growth Capacity on Formate

Advanced Energy and Sustainability Research, Volume 7, Issue 5, May 2026.
Engineering Yeast for Growth on Formate and Methanol via Serine‐Centered C1 Coupling. By engineering serine metabolism and remodeling the rGly pathway, we rendered yeast growth strictly dependent on formate. This synthetic formatotrophy enables growth on dual‐C1 substrates by channeling formate into the central metabolism via serine deaminases and ...
Shuyuan Guo, Gulikezi Maimaitirexiati, Qiannan Zhang, Zhehao Jin, Kaidi Chen, Tiantian Zou, Xuemei Zhao, Xiangjian Liu, Zhenmin Bai, Dashuang Gao, Xudong Kong, Tao Yu   +11 more
wiley   +1 more source

Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris

Microbiology Indonesia, 2017
Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57
doaj   +1 more source

Citrobacter amalonaticus phytase on the cell surface of Pichia pastoris exhibits high pH stability as a promising potential feed supplement. [PDF]

PLoS ONE, 2014
Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst.
Cheng Li, Ying Lin, Yuanyuan Huang, Xiaoxiao Liu, Shuli Liang   +4 more
doaj   +1 more source

Nanobiocatalysts: Potential Applications in Biofuel Production and Biotransformation

Carbon Neutralization, Volume 5, Issue 3, May 2026.
The application of nanobiocatalysts in the field of biofuel production and the biotransformation of substrate were reviewed. Further, the possible research gaps in the field of biofuel and value‐added compound production and a possible way to enhance the application of nanobiocatalyst in future development are highlighted.
Preethi Muthu, Kalaiselvi Thiyagarajan, V. Pugalenthi, M. Gunasekaran, J. Rajesh Banu   +4 more
wiley   +1 more source