Data in Brief, 2015 This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P.
Kerstin Lange +3 more
doaj +1 more source
Functional expression of a blood tolerant laccase in Pichia pastoris [PDF]
, 2013 Background: Basidiomycete high-redox potential laccases (HRPLs) working in human physiological fluids (pH 7.4, 150 mM NaCl) arise great interest in the engineering of 3D-nanobiodevices for biomedical uses.
Alcalde, Miguel +4 more
core +3 more sources
Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics
FEBS Open Bio, Volume 16, Issue 4, Page 653-669, April 2026.Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini +2 more
wiley +1 more source
Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris
Current Research in BiotechnologyPichia pastoris is one of the most commonly used hosts for producing heterologous proteins, whereas production levels vary depending on the protein of interest and are also regulated by regulatory factors.
Shengyan Wang +6 more
doaj +1 more source
Systematically Engineering for Efficient Production of 3‐Methyl‐1‐Butanol in Escherichia coli
Advanced Science, Volume 13, Issue 21, 13 April 2026.An integrated metabolic engineering strategy was established for high‐level 3‐methyl‐1‐butanol biosynthesis in Escherichia coli. Molecular dynamics‐guided semi‐rational engineering of dihydroxyacid dehydratase uncovered and relieved key catalytic bottlenecks, while adaptive laboratory evolution enhanced strain robustness.
Nanfei Geng +6 more
wiley +1 more source
Applied Microbiology and Biotechnology / The bud tip is the cellular hot spot of protein secretion in yeasts [PDF]
, 2016 in deutscher Sprache nicht verf\ufcgbarYeasts are valuable hosts for recombinant protein production. Among them, Pichia pastoris is frequently used for production of secreted proteins, and much effort was made to improve the secretion efficiency of this ...
Gasser, Brigitte +2 more
core +1 more source
Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris [PDF]
, 2016 BACKGROUND: Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production.
Jiangchao Qian +7 more
core +1 more source
Characterisation of a recombinant β-xylosidase (xylA) from Aspergillus oryzae expressed in Pichia pastoris [PDF]
, 2014 β-xylosidases catalyse the hydrolysis of short chain xylooligosaccharides from their non-reducing ends into xylose. In this study we report the heterologous expression of Aspergillus oryzae β-xylosidase (XylA) in Pichia pastoris under the control of the ...
A Dilokpimol +24 more
core +3 more sources
High expression of human carboxypeptidase M in Pichia pastoris. Purification and partial characterization [PDF]
, 2006 Carboxypeptidase M (CPM) is an extracellular glycosylphosphatidylinositol-anchored membrane glycoprotein, which removes the C-terminal basic residues, lysine and arginine, from peptides and proteins at neutral pH.
Araujo, Ronaldo Carvalho [UNIFESP] +7 more
core +1 more source
Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches [PDF]
, 2017 Background. Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production.
Bakar, F. D. A. +6 more
core +3 more sources