Results 111 to 120 of about 21,587 (226)

Measuring intracellular calcium concenrations in Pichia pastoris

open access: yes, 2013
Pichia pastoris, a methylotrophic yeast, is used for heterologous protein expression. Calcium is an important second messenger in signal transduction. Little research has been performed to analyze calcium homeostasis in P. pastoris.
Kim, Joseph   +3 more
core  

Influencia de la fuente de carbono sobre la expresión de proteínas AOX1-reguladas en Pichia pastoris

open access: yes, 2005
La levadura Pichia pastoris constituye un excelente modelo para la expresión de proteínas heterólogas, lo que se refleja en el gran número de proteínas que han sido obtenidas en este modelo biológico, bajo el control del promotor AOX1.
Homero Sáenz Sáenz   +19 more
core   +1 more source

High-quality genome sequence of Pichia pastoris CBS7435

open access: yes, 2011
Küberl A, Schneider J, Thallinger GG, et al. High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology. 2011;154(4):312-320.The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly
Szczepanowski, Rafael   +27 more
core   +1 more source

Supersecretion in Pichia pastoris mutant strains

open access: yes, 2017
Pichia pastoris is a methylotropic yeast that is commonly used for the secretion of large amounts of heterologous protein products. Continuous progress towards increasing secretion of protein products in Pichia pastoris is important for commercial and ...
Afnan, Mina   +4 more
core  

Pichia pastoris biomass production from biodiesel waste

open access: yes, 2012
Badano zdolność drożdży Pichia pastoris ATCC 28485 do wzrostu w podłożu o zróżnicowanym pH, zawierającym jako źródło węgla surowce odpadowe z produkcji biodiesla.
Juszczyk, P., Rymowicz, W., Liszka, P.
core  

Producción de una fitasa recombinante en Pichia pastoris [PDF]

open access: yes, 2007
En el presente trabajo se construyeron cepas recombinantes de Pichia pastoris capaces de producir y secretar una fitasa de origen bacteriano, factible de ser empleada como aditivo en alimentos para animales.
Rodríguez Blanco, Lili   +3 more
core  

Use of a dual biosensor for identification of novel secretion signal peptides and efficient screening of precision fermentation production strains.

open access: yesFuture Foods
Methylotrophic yeast Pichia pastoris (Komagaetella phaffii) has emerged as a versatile and robust host for recombinant protein production, owing to its capacity for high-level protein expression, scalability, and post-translational protein modifications.
Parastoo Karimian   +6 more
doaj   +1 more source

Production of bioactive human alpha-defensin 5 in Pichia pastoris

open access: yes, 2010
[[abstract]]Human alpha-defensin 5 (HD5), a small cationic peptide, is expressed in Paneth cell granules of small intestinal crypts. HD5 exhibits high antimicrobial activity against a broad spectrum of pathogenic agents, including bacteria, fungi, and ...
Hsu, KH (Hsu, Kung-Hao); Pei, C (Pei, Chi); Yeh, JY (Yeh, Jan-Ying); Shih, CH (Shih, Chung-Hsung); Chung, YC (Chung, Yun-Chin); Hung, LT (Hung, Lien-Tsung); Ou, BR (Ou, Bor-Rung)
core  

SECRETORY EXPRESSION OF THE EXO-INULINASE FROM K. MARXIANUS CBS 6556 IN PICHIA PASTORIS

open access: yes, 2008
Inulin is linear oligosaccharides [1]. Although inulin can be hydrolyzed to monosaccharides non-enzymatically by acid treatment, inhibitory by-products produced that complicate downstream biological process.
王倩, 杨帆, 赵宗保, 张素芳
core  

Comparative analysis of expression levels of recombinant antibody fragments vNAR in Pichia pastoris and Escherchia coli

open access: yes, 2014
Los fragmentos de anticuerpo de dominio sencillo (~12 kDa), han mostrado ventajas sobre las moléculas de anticuerpo convencionales (~150 kDa) respecto a su aplicación clínica.
Olivia Cabanillas Bernal
core  

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