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Electroporation of Pichia pastoris
2014The introduction of foreign DNA into the yeast Pichia pastoris is similar to the processes used with Saccharomyces cerevisiae and routinely performed by the electroporation of plasmid DNA into electro-competent (E-comp) P. pastoris cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et
Knut Madden +2 more
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2003
The genus Hansenula (Pichia) belongs to the class Ascomycetes, order Endomycetales, family Saccharomycetaceae. To date, only two species of this genus have been implicated in human disease: H. anomala and H. polymorpha (1–4). H. anomala (also synonymous with Pichia anomala [5,6]), by far the most common human pathogen of the two, is an ascosporogenous ...
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The genus Hansenula (Pichia) belongs to the class Ascomycetes, order Endomycetales, family Saccharomycetaceae. To date, only two species of this genus have been implicated in human disease: H. anomala and H. polymorpha (1–4). H. anomala (also synonymous with Pichia anomala [5,6]), by far the most common human pathogen of the two, is an ascosporogenous ...
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Methods in enzymology, 2009
The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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2017
The modified form of the xylose reductase (XR) of Pichia stipitis with decreased affinity toward NADP??(H???) was created via site-specific mutagenesis. Recombinant strain of P. stipitis overexpressing the mutated XR characterized by 1,3-fold increase in productivity of xylose alcoholic fermentation was constructed.
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The modified form of the xylose reductase (XR) of Pichia stipitis with decreased affinity toward NADP??(H???) was created via site-specific mutagenesis. Recombinant strain of P. stipitis overexpressing the mutated XR characterized by 1,3-fold increase in productivity of xylose alcoholic fermentation was constructed.
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2019
????????????????, ???? ???????????????? ?????????????????????? ?????? Pichia guilliermondii, ???????????? ?????????????? ?????????????????????? ?????? GTP-???????????????????????????? II (RIB??), ?? ???????????? ???????????????????????????? ?????????????? ?????????????????? ???????????? ?????????????? ?????????????????????????? ???????????? ?????????? ?
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????????????????, ???? ???????????????? ?????????????????????? ?????? Pichia guilliermondii, ???????????? ?????????????? ?????????????????????? ?????? GTP-???????????????????????????? II (RIB??), ?? ???????????? ???????????????????????????? ?????????????? ?????????????????? ???????????? ?????????????? ?????????????????????????? ???????????? ?????????? ?
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