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Expression of proteins in Pichia pastoris
2021The methylotrophic yeast Pichia pastoris is currently one of the most versatile and popular hosts for the production of heterologous proteins, including industrial enzymes. The popularity of P. pastoris stems from its ability to grow to high cell densities, producing high titers of secreted heterologous protein with very low amounts of endogenous ...
Giuliana Mastropietro +2 more
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ACS Synthetic Biology, 2021
Microbial production of α-farnesene from renewable raw materials is a feasible alternative to traditional petroleum craft. Recently, the research on improving α-farnesene production in Pichia pastoris mainly focused on cytoplasmic engineering, while ...
Hui Liu +3 more
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Microbial production of α-farnesene from renewable raw materials is a feasible alternative to traditional petroleum craft. Recently, the research on improving α-farnesene production in Pichia pastoris mainly focused on cytoplasmic engineering, while ...
Hui Liu +3 more
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Dynamic Feeding for Pichia pastoris
2022The knowledge of certain strain-specific parameters of recombinant Pichia pastoris strains is required to be able to set up a feeding regime for fed-batch cultivations. These parameters are commonly determined either by time-consuming and labor-intensive continuous cultivations or by several, consecutive fed-batch cultivations. Here, we describe a fast
Alexander, Pekarsky, Oliver, Spadiut
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Green Chemistry, 2021
Establishment of a sustainable non-animal biosynthesis of chondroitin sulfate from methanol using genetically engineered Pichia pastoris.
Xuerong Jin +7 more
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Establishment of a sustainable non-animal biosynthesis of chondroitin sulfate from methanol using genetically engineered Pichia pastoris.
Xuerong Jin +7 more
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Electroporation of Pichia pastoris [PDF]
, 2014 The introduction of foreign DNA into the yeast Pichia pastoris is similar to the processes used with Saccharomyces cerevisiae and routinely performed by the electroporation of plasmid DNA into electro-competent (E-comp) P. pastoris cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et
James M. Cregg +2 more
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Metabolic engineering of Pichia pastoris for malic acid production from methanol
Biotechnology and Bioengineering, 2020The application of rational design in reallocating metabolic flux to accumulate desired chemicals is always restricted by the native regulatory network.
Feng Guo +9 more
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2019
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is used as an expression system for recombinant protein production for a variety of applications. It grows rapidly on inexpensive media containing methanol, glucose, glycerol, or ethanol as a sole carbon source. P.
Semiramis Yılmaz +2 more
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The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is used as an expression system for recombinant protein production for a variety of applications. It grows rapidly on inexpensive media containing methanol, glucose, glycerol, or ethanol as a sole carbon source. P.
Semiramis Yılmaz +2 more
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Chapter 15 Pexophagy in Pichia pastoris
2008The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide Oku, Yasuyoshi Sakai
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Optimising Pichia pastoris Induction
2012A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields ...
Zharain Bawa, Richard A. J. Darby
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Glycosylation in Pichia pastoris
Biotechnology and Applied Biochemistry, 1999The Pichia pastoris system for expression of heterologous recombinant proteins is being used increasingly because of the large yields of properly folded proteins that result and the ease of scaling preparations into large-biomass fermentors. Another advantage of this system centres on the type of glycosylation that results, generally yielding protein ...
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