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Das Pichia pastoris-Expressionssystem
2012Die methylotrophe Hefe Pichia pastoris (P. pastoris, Abb. 15–1) wurde in den letzten Jahren weltweit erfolgreich als System zur heterologen Genexpression etabliert und gehort mittlerweile neben Escherichia coli (Kap. 14), Saccharomyces cerevisiae, Hansenula polymorpha, dem Baculovirus-Expressionssystem (Kap. 14), Saugerzellsystemen (Kap.
Reinhart, C., Krettler, C.
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ภาวะเหมาะที่สุดของการผลิตรีคอมบิแนนต์อินซูลินในยีสต์ Pichia pastoris KM71H
2023ปัจจุบันมีจำนวนของผู้ป่วยโรคเบาหวานเพิ่มสูงอย่างต่อเนื่องทั้งในระดับโลกและระดับชาติ ในประเทศไทย วิกฤตนี้ได้ส่งผลให้เกิดแรงผลักดันในการผลิตอินซูลินขึ้นภายในประเทศเอง ซึ่งมีจุดประสงค์เพื่อบำบัดรักษาผู้ป่วยโรคเบาหวาน และเพื่อหลีกเลี่ยงการนำเข้าอินซูลินจากต่างประเทศด้วย อินซูลินที่ใช้ในการบำบัดรักษาผู้ป่วยนั้นสามารถผลิตได้จากหลายวิธี อาทิ การสกัดจากตับอ ...
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Glycosylation in Pichia pastoris
Biotechnology and Applied Biochemistry, 1999The Pichia pastoris system for expression of heterologous recombinant proteins is being used increasingly because of the large yields of properly folded proteins that result and the ease of scaling preparations into large-biomass fermentors. Another advantage of this system centres on the type of glycosylation that results, generally yielding protein ...
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Towards systems metabolic engineering in Pichia pastoris
Biotechnology Advances, 2017The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of recombinant proteins, frequently outperforming other heterologous hosts. Already, a sizeable amount of systems biology knowledge has been acquired for this non-conventional yeast.
Jan-Philipp Schwarzhans +2 more
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Electroporation of Pichia pastoris
2014The introduction of foreign DNA into the yeast Pichia pastoris is similar to the processes used with Saccharomyces cerevisiae and routinely performed by the electroporation of plasmid DNA into electro-competent (E-comp) P. pastoris cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et
Knut Madden +2 more
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Cell-Free Protein Synthesis Using Pichia pastoris
2022Pichia pastoris (syn. Komagataella phaffii) is an industrially relevant recombinant protein platform that has been used to produce over 5000 proteins to date. Cell-free protein synthesis can be used as a screening tool before strain development or for the production of proteins that are difficult or toxic to make in vivo.
Alex J, Spice +2 more
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Heterologous interferons synthesis in yeast Pichia pastoris
Applied Biochemistry and Microbiology, 2010The HuIFNA16, HuIFNB, and BoIFNG genes encoding human [alpha]16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed.
M V, Padkina +3 more
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Glycosylation of Pichia pastoris ‐derived proteins
Biotechnology and Applied Biochemistry, 1999The Pichia pastoris system for expression of heterologous recombinant proteins is being used increasingly because of the large yields of properly folded proteins that result and the ease of scaling preparations into large‐biomass fermentors. Another advantage of this system centres on the type of glycosylation that results, generally yielding protein ...
R K, Bretthauer, F J, Castellino
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Gene expression in yeast: Pichia pastoris
Current Opinion in Biotechnology, 1991Recent studies have shown the versatility and utility of the Pichia pastoris expression system. Improvements in strains have boosted the yield of proteins and peptides to the commercially feasible range. The Pichia pastoris expression system will soon be used to manufacture proteins for human clinical trials.
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Bax-induced cell death in Pichia pastoris
Biotechnology Letters, 1999Murine Bax was expressed in the methylotrophic yeast, Pichia pastoris, using the alcohol oxidase 1 (AOX1) or alcohol oxidase 2 (AOX2) promoter and the AOX1 terminator. Upon induction in methanol medium, transformants containing BAX cDNA under control of the strong AOX1 promoter showed complete growth inhibition and extensive cell death.
Martinet, Wim +4 more
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