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Optimization of the Expression of Equistatin in Pichia pastoris
Protein Expression and Purification, 2002To improve the expression of equistatin, a proteinase inhibitor from the sea anemone Actinia equina, in the yeast Pichia pastoris, we prepared gene variants with yeast-preferred codon usage and lower repetitive AT and GC content. The full gene optimization approximately doubled the level of steady-state mRNA and protein accumulated in the culture ...
Outchkourov, N.S. +2 more
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Recombinant protein expression in Pichia pastoris
Molecular Biotechnology, 2000The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool.
David Higgins +3 more
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Expression of Recombinant Proteins in Pichia Pastoris
Applied Biochemistry and Biotechnology, 2007Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression.
Kuppusamy Ilangovan +9 more
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Chapter 13 Expression in the Yeast Pichia pastoris
2009The yeast Pichia pastoris has become the premier example of yeast species used for the production of recombinant proteins. Advantages of this yeast for expression include tightly regulated and efficient promoters and a strong tendency for respiratory growth as opposed to fermentative growth.
Knut Madden +5 more
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Engineering Pichia pastoris for the Production of Carotenoids
2018Carotenoids are one of the most diverse and widely distributed classes of pigments in the biosphere and exhibit a variety of functions in the nature. Their importance and biotechnological applications are higher and higher, but their sources are not increasing in the same exponential way.
Patricia Veiga-Crespo +2 more
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Expression of Recombinant Fungal Proteins in Pichia Pastoris
2021Production of recombinant proteins, including enzymes, in a heterologous host is often necessary to obtain sufficient amounts for functional studies. In particular, yeast expression systems are cost-effective and convenient for producing recombinant proteins having post-translational modifications characteristic of eukaryotes. The yeast Pichia pastoris
Kiyohiko Igarashi, Naoki Sunagawa
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Biosynthesis of catharanthine in engineered Pichia pastoris
Nature Synthesis, 2023Jucan Gao +14 more
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Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production
Critical Reviews in Biotechnology, 2019Pichia pastoris is extensively used to produce various heterologous proteins. Amounts of biopharmaceutical drugs and industrial enzymes have been successfully produced by fed-batch high-cell-density fermentation (HCDF) of this cell factory.
Wan-Cang Liu +5 more
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Das Pichia pastoris-Expressionssystem
2012Die methylotrophe Hefe Pichia pastoris (P. pastoris, Abb. 15–1) wurde in den letzten Jahren weltweit erfolgreich als System zur heterologen Genexpression etabliert und gehort mittlerweile neben Escherichia coli (Kap. 14), Saccharomyces cerevisiae, Hansenula polymorpha, dem Baculovirus-Expressionssystem (Kap. 14), Saugerzellsystemen (Kap.
Christoph Krettler, Christoph Reinhart
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