Results 241 to 250 of about 44,813 (273)
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Methods in enzymology, 2009
The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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Expression of Recombinant Proteins in Pichia Pastoris
Applied Biochemistry and Biotechnology, 2007Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression.
Pingzuo, Li +6 more
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Crystallization of alcohol oxidase from Pichia pastoris
Journal of Molecular Biology, 1989Crystals of alcohol oxidase purified from Pichia pastoris were grown in microdialysis buttons in a solution of polyethylene glycol, sodium chloride and sodium azide. The crystals were stratified along the major axis and up to 3 mm in length. X-ray diffraction experiments indicated a space group of P2(1) and unit cell dimensions of a = 157.3 A, b = 171 ...
C W, Boys +3 more
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Crystallization ofPichia pastorislysyl oxidase
Acta Crystallographica Section D Biological Crystallography, 2002A copper-containing amine oxidase (PPLO) from the yeast Pichia pastoris has been purified and crystallized in two forms. PPLO is a glycoprotein. The molecular mass from SDS-polyacrylamide gels is 112 kDa, consistent with 20% glycosylation by weight (the calculated molecular weight of the polypeptide is 89.7 kDa).
Mihwa, Lee +9 more
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Recombinant Batroxobin Expressed Highly in Pichia pastoris
Chinese Journal of Biotechnology, 2007Methylotrophic yeast, Pichia pastoris was used to express recombinant batroxobin, and a technology route of producing recombinant protein was finally established. We synthesized batroxobin gene artificially by means of recursive PCR. pPIC9-batroxobin was constructed and transformed into Pichia pastoris GS115 (his4). Recombinant batroxobin was expressed
Zhao-Fa, Li +4 more
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Pichia pastoris Mayasında Heterolog Protein Üretimi.
2010Pichia pastoris, Saccharomycetes familyasına ait rekombinant protein üretebilen, moleküler biyoloji ve biyoteknoloji çalışmalarında önemli metilotrofik bir mayadır.Pichia pastoris, genetik yönlendirmeye açık, hücre içine ve dışına yüksek oranda protein eksprese edebilen, yüksek ökaryotlarda ...
HIZ, MELİHA MERVE, AKI, CÜNEYT
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2021
Aim. Fructose-1,6-bisphosphatase (FBPase) which is synthesized when cells are grown on non-fermentable carbon sources. When yeast cells are subsequently shifted to a glucose-containing medium, FBPase is rapidly inactivated and degraded. This process is called catabolite degradation. Methods.
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Aim. Fructose-1,6-bisphosphatase (FBPase) which is synthesized when cells are grown on non-fermentable carbon sources. When yeast cells are subsequently shifted to a glucose-containing medium, FBPase is rapidly inactivated and degraded. This process is called catabolite degradation. Methods.
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การแสดงออกและลักษณะสมบัติของฮีมแอกกลูทินินจากไวรัสไข้หวัดนก (H5N1) ใน Pichia pastoris
เชื้อไวรัสไข้หวัดนกสายพันธุ์ H5N1 เป็นเชื้อไวรัสที่ก่อให้เกิดโรคไข้หวัดใหญ่ในสัตว์ปีก ซึ่งเป็นโรคที่แพร่กระจายอย่างรวดเร็ว และเป็นสาเหตุให้เกิดความเสียหายทางด้านเศรษฐกิจและสาธารณสุข โปรตีนฮีมแอกกลูทินินเป็นโปรตีนที่มีความสำคัญอย่างยิ่งต่อวงจรชีวิตของไวรัส เพราะฮีมแอกกลูทินินเป็นโปรตีนที่จะจับกับตัวรับของเซลล์เจ้าบ้าน ทำให้เข้าสู่เซลล์และเกิดการจำลองต ...openaire +1 more source
ภาวะที่เหมาะสมของภาวะการเลี้ยงเชื้อเพื่อผลิตซีโครปิน เอ ในรีคอมบิแนนต์ Pichia pastoris
ซีโครปิน เอ (cecropins A, CA) เป็นเพปไทด์ต้านจุลินทรีย์ที่พบในระบบภูมิคุ้มกันในน้ำเลือด (immune hemolymph) ของผีเสื้อกลางคืน Hyalophora cecropia มีฤทธิ์ฆ่าจุลินทรีย์ได้ทั้งแกรมบวก และ แกรมลบ รวมถึงมีฤทธิ์ในการยับยั้งการเกิดเนื้องอกและต้านเซลล์มะเร็ง อย่างไรก็ตามการสกัด ซีโครปิน เอ จากผีเสื้อกลางคืนทำได้ยากเนื่องจากจะมีการปนเปื้อนของโปรตีนที่ไม่ต ...openaire +1 more source