Purified zymogens reveal mechanisms of snake venom metalloproteinase auto-activation [PDF]
eLifeSnake venoms contain diverse mixtures of toxins that evolved to incapacitate prey, but in humans, they cause extensive pathology following snakebite envenomation. In viper venom, some of the most potent toxins are the haemorrhagic and coagulopathic snake
Sophie Hall +17 more
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Spatial phosphoproteomics reveals regional phosphorylation features in liver cirrhosis progression [PDF]
Journal of Translational MedicineBackground Liver cirrhosis (LC) is the end stage of chronic liver disease and is characterized by pseudolobule formation and extensive fibrosis. Protein phosphorylation, a key posttranslational modification, regulates cellular functions and disease ...
Mengyao Wu +12 more
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Temperature changes are signaled in cyanobacteria through the PipX interaction network [PDF]
Frontiers in MicrobiologyCyanobacteria perform oxygenic photosynthesis and have evolved sophisticated mechanisms to adapt their metabolism to challenging environmental changes.
Antonio Llop +8 more
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A nanodiamond-formulated plant protein induces robust immunity against porcine epidemic diarrhea virus in piglets [PDF]
Frontiers in ImmunologyPorcine epidemic diarrhea virus (PEDV) continues to be a major infectious threat in swine, especially endangering piglets. The COE and S1D domains have been identified as crucial antigens suitable for designing subunit vaccines. Nanodiamonds (NDs), owing
Thuong Thi Ho +14 more
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Post-translational modification of PII signal transduction proteins
Frontiers in Microbiology, 2015 The PII proteins constitute one of the most widely distributed families of signal transduction proteins in nature. They are pivotal players in the control of nitrogen metabolism in bacteria and archaea, and are also found in the plastids of plants. Quite
Mike eMerrick
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PII Protein-Derived FRET Sensors for Quantification and Live-Cell Imaging of 2-Oxoglutarate [PDF]
Scientific Reports, 2017 The citric acid cycle intermediate 2-oxoglutarate (2-OG, a.k.a. alpha-ketoglutarate) links the carbon and nitrogen metabolic pathways and can provide information on the metabolic status of cells. In recent years, it has become exceedingly clear that 2-OG
Jan Lüddecke +8 more
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Tuning the in vitro sensing and signaling properties of cyanobacterial PII protein by mutation of key residues. [PDF]
Sci Rep, 2019 Selim KA +3 more
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Role of PII proteins in nitrogen fixation control of
BMC Microbiology, 2011 Background The PII protein family comprises homotrimeric proteins which act as transducers of the cellular nitrogen and carbon status in prokaryotes and plants.
Steffens Maria BR +7 more
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The complex assembly of N-acetyl-L-glutamate kinase with the PII signal transduction protein follows a two-step mechanism [PDF]
PII signal transduction proteins control central carbon/nitrogen metabolism via interacting with and modulating many cellular targets. In photoautotrophs, N-acetyl-L-glutamate kinase (NAGK), the rate-limiting enzyme of arginine biosynthesis, is the ...
Abdalla A. Elshereef +2 more
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Cryo-EM structure of a methanogen nitrogenase–PII protein supercomplex [PDF]
ABSTRACT Nitrogenases are metalloenzymes that catalyze the reduction of atmospheric dinitrogen to ammonia, sustaining the global nitrogen cycle. While bacterial nitrogenase has been extensively characterized, the architecture and regulation of archaeal nitrogenases remain unknown despite longstanding evidence of ...
R. P. KASHYAP +7 more
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