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Structure-Function Relationship of Bromelain Isoinhibitors from Pineapple Stem
Biological Chemistry, 2002Bromelain isoinhibitors from pineapple stem (BIs) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. The three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet.
Ken-ichi, Hatano +2 more
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Ananain: A novel cysteine proteinase found in pineapple stem
Archives of Biochemistry and Biophysics, 1988A previously unknown cysteine proteinase, named ananain, has been isolated from crude commercial pineapple stem bromelain. The purification procedure involved affinity chromatography on Sepharose-Gly-Phe-glycinaldehyde semicarbazone, and cation-exchange chromatography. The relative molecular mass of ananain was very similar to that of bromelain (25,000
A D, Rowan, D J, Buttle, A J, Barrett
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Characterization and Structure of Pineapple Stem Inhibitor of Cysteine Proteinases
Biological Chemistry Hoppe-Seyler, 1992The complete amino acid sequence of the inhibitor of cysteine proteinases from pineapple stem acetone powder was determined. The inhibitor consists of 52 amino acids and is composed of two polypeptide chains (41 and 11 amino acids) linked via disulphide bonds. It differs from already known sequences in one to four amino acids.
B, Lenarcic +4 more
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Antioxidant, Antimicrobial, 15‐LOX, and AGEs Inhibitions by Pineapple Stem Waste
Journal of Food Science, 2011Abstract: Pineapple stem has been extensively used for bromelain extraction; however, almost no attention has been given to the waste obtained during bromelain manufacturing. In this regard, antioxidant, antimicrobial, and inhibitions against 15‐lipoxygenase and advanced glycation end product formations by ...
Atul, Upadhyay +3 more
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Stem bromelain—A new protease preparation from pineapple plants
Economic Botany, 1957The proteolytic enzymes in this plant product, not yet in commercial production, may find application, as do similar agents from other sources, in the bating of hides, tenderizing of meat, chill-proofing of beer and other directions suggested in this article.
R. M. Heinicke, W. A. Gortner
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Bromein, a Bromelain Inhibitor from Pineapple Stem: Structural and Functional Characteristics
Protein & Peptide Letters, 2018Bromelain inhibitor, "bromein", is a proteinase-inhibitor specific to the cysteine proteinase bromelain from pineapple stem. In the stem, eight bromein isoforms are known to exist, and each isoform has a short peptide (light chain) and a long one (heavy chain) with five disulfide bonds.
Ken-Ichi, Hatano +2 more
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PREPARATION AND CHARACTERIZATION OF THERMOPLASTIC STARCH FROM PINEAPPLE STEM: EFFECT OF PLASTICIZERS
Suranaree Journal of Science and Technology, 2023This work studied the effect of types and amount of plasticizers on thermoplastic starch properties from pineapple stem with good mechanical properties, water resistant and biodegradable. Three plasticizers were studied: erythritol, xylitol, and sorbitol. Thermoplastic starch film was prepared by solution casting.
Siraprapha Yeaprayoon +3 more
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Characteristics and potential utilizations of starch from pineapple stem waste
Industrial Crops and Products, 2017Abstract Pineapple stems have become a significant agricultural waste in Thailand because pineapple has to be replanted every two years. However this renewable waste resource can be a good source of starch. This study is aimed to extract and characterize the starch using the common mechanical extraction with water.
Nithima Nakthong +2 more
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Pineapple stem bromelain immobilized on different supports: Catalytic properties in model wine
Biotechnology Progress, 2012AbstractBromelain from pineapple stem has been covalently immobilized on different supports to select the more efficient biocatalyst that should be applied toward unstable proteins in real white wine. In this preliminary study, catalytic properties of different immobilized bromelain forms were compared under wine‐like conditions, against a synthetic ...
Benucci, Ilaria +3 more
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In Vivo Antitumoral Activity of Stem Pineapple (Ananas comosus) Bromelain
Planta Medica, 2007Stem bromelain (EC 3.4.22.32) is a major cysteine proteinase, isolated from pineapple ( Ananas comosus) stem. Its main medicinal use is recognized as digestive, in vaccine formulation, antitumoral and skin debrider for the treatment of burns. To verify the identity of the principle in stem fractions responsible for the antitumoral effect, we isolated ...
Roxana, Báez +3 more
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